vero cells 中文意思是什麼
-
In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv
理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。 -
Dna amage caused by mercury chloride in vero cells and effect of zinc sulfate and sodium selenite on antagonism to this dna amage
中藥和葡萄糖酸鋅治療兒童缺鋅性厭食92例 -
Then were electrophored. the extent of dna migration were measured. index - percentage of " comet " cell and " comet tail " were analysed that indicated when vero cells were treated with 2 u g / ml quinocetone vero cells got midium - grade damage, and were treated with 6 u g / ml olaquindox vero cells got midium - grade damage
喹乙醇染毒劑量在2 10 g ml時細胞存活率90 ，以2 10 g ml劑量的喹乙醇染毒處于對數生長期的vero細胞，后經過電泳，通過分析彗星樣細胞發生率和彗尾長短等指標，結果表明染毒劑量在6 g ml對dna造成中度損傷。 -
Index - percentage of " comet " cell and " comet tail " were analysed that indicated the optimum time is 2 ~ 3h. the degree of dna damage by quinocetone and olaquindox were detected by scge assay, when vero cells were treated with 1 ~ 5 ug / ml quinocetone the percentage of live cells was above 85 % and with 2 - 10ug / ml olaquindox the percentage of live cells was above 90 %
喹烯酮染毒劑量在1 5 g ml時細胞存活率85 ，以1 5 g ml劑量的喹烯酮染毒處于對數生長期的vero細胞，后經過電泳，通過分析彗星樣細胞發生率和彗尾長短等指標，結果表明染毒劑量在2 g ml對dna造成中度損傷。 -
Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa
將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。 -
Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque
利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。 -
Expression of the infectious bursal disease virus polyprotein in vero cells using attenuated salmonella typhimurium as transgenic carrier
細胞中表達傳染性法氏囊病病毒多聚蛋白基因 -
We applied single cell gel electrophoresis and cell culture technique, which constitute sing cell gel electrophoresis assay system for detecting mutagenicity to detect mutagenesis in vitro induced by animal drug quinocetone and olaquindox. and confirmed optimum lysing - time. vero cells in the period of logarithm - growth time were treated with 9. 1 ~ 273u mol / l h2o2 at 37 3h, then were lysed for lh, 2h, 3h and 4h to find optimum lysing - time
並基於陽性致突變物h _ 2o _ 2作用於非洲綠猴腎vero細胞引起細胞dna損傷的原理，研究了其關鍵步驟-裂解時間，以9 . 1 273 mol l劑量的h _ 2o _ 2染毒處于對數生長期的vero細胞3h后，收獲細胞用於制備三明治凝膠板，分別裂解1h 、 2h 、 3h和4h並選擇最適裂解時間。 -
The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza
結果表明所獲得的重組病毒（ rprvha ）遺傳性狀穩定，在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白，為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。 -
An expression vector with fragment 9 in antisense orientation was constructed to block the expression of the relevant gene ( fragment 9 related gene, fnr gene ) in vero cells. interestingly, we found that the nontargeted mutation frequency induced by mnng was increased significantly, implicating that the product of the blocked gene may be involved in the inhibition of nontargeted mutation
利用反義核酸技術構建含反向插入9號片段的真核細胞表達重組體並轉染細胞，以獲得反義rna阻斷vero細胞中相應基因的表達，發現mn 』 ng誘發的非定標性突變頻率顯著增高，提示被阻斷的相關基因的表達產物可能參與抑制非定標性突變的發生。 -
In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。 -
The immune effect and systemic reaction of the freeze - derived inactivated japanese encephalitis vaccine made from vero cells in an epidemic area
細胞流行性乙型腦炎滅活疫苗在流行區的人體反應及免疫效果 -
Studies on clinical trials and immune effect of a new purified rabies vaccine prepared from vero cells
細胞狂犬病疫苗的臨床觀察及免疫學效果研究
分享友人