yac 中文意思是什麼

yac 解釋
酵母人工染色體基因
  1. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測,從864個轉化子中獲得了4個陽性克隆,測序表明,人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上,並獲得了可進行細胞融合的重組yac載體。
  2. But the routine methods of yac transgene transfer such as microinjection cannot introduce yac into livestocks by now

    但目前還沒有報道用常規的yac轉基因方法能成功的將yac導入大動物體內的例子。
  3. To clone large and random dna fragments, the second generation of yac, pjs97 - pjs98 was modified as gene replacement vector in streptomyces

    4kb和1 . okb片段,而且還插入了勿,七刃j和spc心t廠抗性基因。
  4. It have been reported that the 210 kb yeast artificial chromosome ( yac ) including the human a - lactalbumin gene could direct the transgene to express in a position - independent manner, therefore is an excellent expression vector to produce transgenic animal bioreactors

    根據報道含完整人-乳白蛋白基因的yac載體能指導外源基因的乳腺特異性高效表達,是生產動物乳腺生物反應器的理想表達載體。
  5. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此,本試驗首先擴增出整合在酵母基因組里的人血清白蛋白( hsa )基因作為目的基因,並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體,以構建整合型載體,再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  6. In order to construct plant artificial chromosome, plasmid vectors have been constructed to integrate necessary elements into both right and left yac arms

    為了構建植物人工染色體,我們構建了可以和含有著絲粒序列的yac載體的左臂和右臂進行同源重組的質粒載體。
  7. Because of its fully sequenced genome and the most complete, contiguous nucleotide sequence of centromeric dna in plants, arabidopsis was chose as the base to construct plant artificial chromosome. we identified two candidate yac clones, cic8h8 and cic6f6, which are both proved to have the 178 bp arabidopsis centromeric repeat sequences

    我們通過pcr和序列分析,選擇了含有擬南芥著絲粒區178bp串連重復序列的yac克隆作為構建人工染色體的基礎,同時也克隆了擬南芥的端粒和ars等序列。
  8. The chief aim of the present study is trying to extend the applications of yac and scp2 *, which have tremendous cloning capacity, for the above mentioned purpose. streptomyces sp. fr - 008 pks gene cluster ( c

    本文對yac和scp2 *這類承載能力較大的載體系統在克隆fr - 008抗生素合成基因簇方面的利用進行了一系列載體改造和克隆策略方面的初步探索。
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