zona reaction 中文意思是什麼
zona reaction
解釋
透明帶反應-
Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control
目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響，探討內毒素影響體外受精結局的環節及可能的機制，確定三個體外培養系統對內毒素的敏感性，以期為臨床和實驗室質量控制提供參考。 -
In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。
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