參比蛋白 的英文怎麼說

中文拼音 [shēnbǐdànbái]
參比蛋白 英文
reference protein
  • 參 : 參構詞成分。
  • 比 : Ⅰ動詞1 （比較; 較量高下、 長短、距離、好壞等） compare; compete; contrast; match; emulate 2 （比...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Tn order to eliminatc the error caused hylight power drift of incident light, we adopt the method of the ration of scattering light to measure the concentration of protein in milk. that is to measure 90 " scattering light intensity and 0 " transmitting light intensity in the light incident plane. the ratio of them is to be used to express measured optical parameter

    由於牛乳蛋白質的測量精度要求很高，為了消除因入射光的光功率漂移而引起的誤差，我們採用散透比法來測量牛乳蛋白質的含量，即在光的入射平面內同時90處的散射光光強is和測量0處的透射光光強it ，用它們的比值來表徵測試牛乳蛋白質含量的光學參量。
  4. Quantify the relevance hydrophobicity in protein structure : the general expectation is that hydrophobic aminoacids are in the core of proteins, while polar aminoacids are on the surface

    量化蛋白質結構的參比疏水性：一般認為，疏水性氨基酸在蛋白質的核中，而極性氨基酸在表面。
  5. Among sequenced 16 positive clones randomly selected, two represent novel expression tag sequences, two are homologous to two unknown proteins in genbank ; the rest are homologous to known or putative proteins or enzymes with definite functions by searching the genbank through blast program, which are involved in various life activities of cell such as regulation of gene expression, plant secondary metabolism, signal transduction, adversity resistance, stress response and defense reaction. significant changes of quantities of these gene fragments were observed before and after ssh, which indicated they were enriched after ssh

    隨機挑選了16個差異表達的克隆進行序列測定，經與genbank數據庫相關數據比較分析，發現有2個新的cdna片段（ ets ） ，有12序列與genbank中已知蛋白或推測蛋白質（ putativeprotein ）序列有高低不同的同源性，它們參與基因的表達調控、植物的次生代謝、信號傳導、抗逆、應激及防禦反應等細胞生命活動過程。
  6. This domain receives the signal from the sensor partner in bacterial two - component systems. these results indicated that the protein of the orf4 works as atpase function involved in the synthesis of magnetosome or takes part in the signal transduction relate to the promotion of magnetosome biosynthesis

    通過對orf4編碼蛋白同源比較和功能分析，推測orf4編碼蛋白可能的功能有兩種：具atpase活性，在磁小體合成過程中為鐵轉運提供能量；或參與磁小體合成啟動過程中的信號轉導。
  7. In this research, we tested 43 herbicides which include 20 different chemical classes and 11 different modes of action using 5 unicellular green algae. the results showed that chlorella pyrenoidosa was more sensitive than the other 4 algae and was suitable as target organism during herbicide screening

    本研究在國內首次以5種單細胞綠藻為模式生物，參照oecd藻類生長抑制測試方法，對包括20種化學結構11種作用機制的43種商品化除草劑進行生物篩選試驗，發現蛋白核小球藻比其他4種藻更適合作為除草劑篩選中的靶標。
  8. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  9. In the course of culture, cell density, content of chlorophyll - a, biomass, content of intracellular protein and intracellular carbohydrate were determined and analyzed, and some other correlative parameters were calculated and compared

    在藻生長過程中，對其細胞數、葉綠素a 、生物量、細胞內蛋白質和糖含量進行測定和分析，並對其它相關參數做了計算和比較。
  10. According to the above, this paper mainly does research on three aspects : using profile - profile method in the process of getting alignment between target sequence and model sequence, data smooth we use in

    最後，我們還參與了casp7的蛋白質結構預測的評比，對獲得的未知序列通過profile - profile方法生成的五個蛋白質結構進行結構合理性評估。
分享友人
分享到 Line

分享到臉書