噬菌體抑制 的英文怎麼說
中文拼音 [shìjūntǐyìzhì]
噬菌體抑制
英文
phage restriction- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 體 : 體構詞成分。
- 抑 : Ⅰ動詞(向下按; 壓制) restrain; repress; curb Ⅱ連詞[書面語]1 (表示抉擇) or 2 (表示轉折) but3 ...
- 制 : Ⅰ動詞1 (製造) make; manufacture 2 (擬訂; 規定) draw up; establish 3 (用強力約束; 限定; 管束...
- 抑制 : 1 (控制) restrain; control; check; hold up; curb; stop; repress; bridle; choke; prehension; sup...
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Under mixed culturing conditions, it was observed that bacterial number rapidly incre ; ised soon after the lysing of host cells. on the contrary, while the non - host cyanobacterium ( i. e. anabaena flos - aquae ) was incubated in the mix culturing system, the breeding of the bacteria could be inhibited. it may be suggested from the result above that cyanophage could bring about the changes in microorganism populations
調查結果顯示, 19個採集的水樣中有6個含有裂解織線藻的噬藻體,而且水樣未經濃縮即能裂解宿主,說明噬藻體在淡水中分佈較廣泛,裂解性也較強;混合培養條件下的研究結果表明,噬藻體裂解宿主后,細菌數量快速增加,而當培養系統中有非宿主藻類存在時,細菌的增殖則受到非宿主藻的抑制,說明噬藻體可以顯著改變系統中微生物的種群結構。Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg )濁對陽性克隆進行限制隴臥賜析( uwi和hedlll )鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率,從中j ) ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。Functions : this product contains “ antifungal active protein ” with optimal and strong sterilization effect, which could strengthen the immunity of body fluid, activate the macrophage, strengthen the phagocytosis ability of macrophage, strengthen the body immunity, restrain the growth, pervasion and transfer of abnormal cells, the product has strong sterilization effect and could strengthen the disease resistance ; it is remarkably effective in improving the immunity and improving the infirm constitution of pets ; the amino acid content and composition in the product are moderate and rational, with the characteristics of strong palatability, nutrition balance and immune element abundance, etc
功能:本產品中擁有極佳強烈殺菌作用的「抗菌活性蛋白」 ,能增強體液免疫功能,活化巨噬細胞,增強其吞噬能力,可增強機體免疫力,抑制非正常細胞生長、擴散和轉移,具有強烈的殺毒作用,增強抗病性;對提高寵物免疫力,改善虛弱體質有顯著效果;其中氨基酸含量適中、組成合理,具有適口性強、營養均衡和免疫物質豐富等特點。By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq
然後,採用噬菌體肽庫技術,以c1q為釣餌蛋白,從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆,經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序,獲得了9個具c1q抑制活性克隆的dna序列,其相應的氨基酸序列為: wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ,它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。And the positive clones were used as the immunogens to immunize mice. both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell )
檢驗這種短肽可否模擬vegf的抗原決定簇誘發機體產生能與vegf結合的抗體,研究小鼠抗血清對huvec (人臍靜脈血管內皮細胞)生長的抑制作用以及噬菌體表達的7肽和12肽對huvec生長的抑制作用。In this study, a novel strategy to inhibit the activation of the complement system has been developed. to get the purified clq as the target molecule for biopanning, the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column
本研究另闢蹊徑,以補體經典激活途徑始動分子c1q為靶標,採用噬菌體肽庫技術,親和篩選能與c1q結合的噬菌體克隆,研製可抑制補體激活的c1q模擬短肽。[ objective ] tumor angiogensis is a critical step for the growth and metastasis of solid tumors. vascular endothelial growth factor ( vegf ) is a specific and potent angiogenic factor that mediates vascularization by binding to the kinase domain receptor ( kdr )
[目的]本實驗試圖通過能中和vegf生物活性的單抗從噬菌體隨機7肽、 12肽文庫中篩選一種能抑制vegf與血管內皮細胞表面受體kdr ( kinasedomainreceptor )結合的vegf模擬短肽。分享友人