堿性桿菌 的英文怎麼說
中文拼音 [jiǎnxìnggǎnjūn]
堿性桿菌
英文
bacillus alcalescens-
Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶( afpga )基因構建表達載體pkkfpga和psmlfpga , pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子,其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子;而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。In order to increase the number of living bacterium, the proper alkali or alkali salt was added to ferment liquid to eliminate stressing effect of organic acid on lactobacillus acidophilus
摘要採用向發酵液中添加適當的堿或堿性鹽等物質的方法,消除了影響嗜酸乳桿菌生長的有機酸的用,達到單位體積的發酵液內含有更多活菌體的目的。Studies on the biological charcteristics of alcaligenes xylosoxidans
木糖氧化產堿桿菌生物學特性研究Bacillus pumilus un - 31 - c - 42 was obtained by mutations of strain ba ( 06 ), which is an alkaline protease producer and separated from life waste in chengdu
短小芽孢桿菌( bacilluspumilus ) un - 42 - c - 31是一株從成都市生活垃圾中分離,經過多次反復誘變后得到的堿性蛋白酶產生菌。The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues, which is part of a alkaline serine protease, with 98. 3 % homogeneity to a reported gene from bacillus pumilus
序列分析顯示,該片段是一個全長1106bp的orf ,可編碼369個氨基酸殘基的多肽,與短小芽孢桿菌堿性蛋白酶基因編碼區高度同源。A pair of primers, based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus, were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf對酶活力有不同程度的抑制作用。以4株芽孢桿菌的總dna為模版,利用擴增短小芽孢桿菌堿性蛋白酶基因的引物對它們進行pcr擴增。The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp. the fermentation liquor also showed good dehairing activity. the alkaline protease ( named dhap, dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography, ion exchange and gel filtration
通過cm - sepharosefastflow離子交換層析, deae - sepharosefastflow離子交換層析, sephacryls - 100 , sephacryls - 200凝膠過濾層析,疏水層析等純化步驟對短小芽孢桿菌發酵液中的堿性蛋白酶進行了純化。The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因,經測序證實與genbank中已發表的序列在編碼區相差2個堿基,其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中,凍融法轉化農桿菌lba4404 ,利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接;用連接產物轉化大腸桿菌jml09感受態細胞,置含amp 、 x - gal和iptg的lb平板上培養12 20小時;挑取白色菌落於選擇性培養基擴大培養,堿裂解法小量提取質粒dna ,並進行酶切分析鑒定,結果獲得整合有prvge基因的重組質粒pugedna ,並與其它prv分離株進行ge基因序列同源性分析。In this research project, the agrobacterium tumefaciens mediated transformation of badh gene of tetraploid black locust has been studied, for the purpose of improving resistance to soline - alkali and drought of tetraploid black locust ; of playing more important role in developing of waste lands, ameliorating of soline - alkali soil, and greening and beautifying of surface - mined lands, mine waste dumps, slopes of roads and railroads where restoration of vegetative cover has proven difficult ; of fully making benefits of its ability to fix atmospheric nitrogen in the soil
為了進一步提高四倍體刺槐的耐鹽性和抗旱性,進一步擴大其適宜種植的生態范圍,充分發揮其固氮、改良土壤的特性,在我國的城鎮綠化、荒山造林、鹽堿地改良以及采礦跡地、公路、鐵路邊坡等植物生長困難土地的植被恢復中發揮其優勢,本實驗對四倍體刺槐進行了農桿菌介導的甜菜堿醛脫氫酶基因轉化的研究。A rare code of arg was in one of the first pair of primer and all of bases of the second pair of primer were changed to biased codes of e. coli
第一對中的一個引物,含有一個精氨酸稀有密碼子,第二對引物則根據密碼子的兼并性,所有堿基都置換成大腸桿菌偏愛的密碼子。Marine low - temperature alkaline protease ( mlap ) is a kind of extracellular enzyme from a kind of marine bacterium of qd80. the study mainly concerned about gene engineering bioinformatics and homology modeling of mlap
海洋低溫堿性蛋白酶( marinelow - temperaturealkalineprotease , mlap )是由一海洋桿菌? qd80產的一種胞外酶。In the same method, another pair of primers weredesigned in order to amplify subtilisin carlsbsrg from genomic dna of those bacillus strains by pcr. the cloning and sequencing of a fragment from bacillus licheniformis dy. are under investigation
又用擴增地衣芽孢桿菌堿性蛋白酶基因的引物在b . licheniformisdy的總dna中擴增到一條與預期大小相一致的片段,進一步實驗仍在進行中。We hold the 40 advanced italian arrow - shaft looms and a 180 printing and dyeing producing line, and we successfully developed the special fabric as followed : flame retardant, flameproof and heat - insulation series, anti - static series, oil resistance and water repellency series, acid and basic resistance series, radiation protection series, dustproof and soil release series, anti - bacteria and odor proof series, without formaldehyde and permanent press series, etc. the features and technical index of our products have reached or exceeded national standard, and that these all have been tested, authenticated and certificated by the national supervising and inspecting center for labour protection articles and shaanxi supervising and inspecting center for textiles quality
在經營常規面料的基礎上,努力致力於新產品的研發推廣和應用,公司以控股形式擁有義大利劍桿織布機40臺, 180印染加工生產線一條,現已開發出:阻燃防火隔熱系列抗靜電系列抗油拒水系列防酸堿系列防電磁波輻射系列防塵易去污系列抗菌防臭系列無甲醛免燙系列等。產品的性能指標均已達到或優于國家標準,並由國家勞動保護用品監督檢驗中心和陜西省紡織品質量監督檢驗中心測試鑒定認可。The human placental alkaline phosphatase ( hplap ) ( ec 3. 1. 3. 1 ) would be used more widely in molecular biology and elisa for its enzyme activity and heat resistant was higher than that of e. coli alkaline phosphatase ( eap ) and calf alkaline phospha - tase ( c1ap ). it is promising to construct the gene engineering pichia pastoris to product the hplap in a scale for the resource of hplap is limited
L )的活性和耐熱性都高於常用的大腸桿菌堿性磷酸酯酶( eap )和小牛腸堿性磷酸酯酶( ciap ) ,因而更適用於分子生物學和酶聯免疫領域,然而其米源受到限制,探討利用畢赤酵母尺屍astorjs構建工程菌進行規模生產鳳有) 』 『闊的前景。Objectives : in our previous study, to improve the expression efficiency of human basic fibroblast growth factor ( hbfgf ) expressed in escherichia coli, we lowered the g + c content in hbfgf ' s tir region and used more preferred codons by e. coli. meanwhile we found the low insoluble product with a large quantity of dimer and higher multimer in the protein solution. it greatly decrease the bioactivity of hbfgf
目的:我們在長期實踐中發現,大腸桿菌中表達的人堿性成纖維細胞生長因子( humanbasicfibroblastgrowthfactor , hbfgf )在e . coli中表達量不高,可溶性低,蛋白溶液中大量存在二聚體,三聚體以至多聚體,使得hbfgf的生物活性大大降低。分享友人