孵育囊 的英文怎麼說
中文拼音 [fūyùnáng]
孵育囊
英文
brood pouch-
Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。Recovery of microtubule organizing centers in enucleated oocytes and condensation of chromosome and organization of spindle in reconstructed embryos were analysed. a non - invasive method of enucleation mouse oocytes has been developed. firstly, the progression of mouse oocytes in meitosis i was ascertained by immunofluoresence technique
另外，本試驗通過在m16基礎上添加eaa 、 non - eaa和葡萄糖顯著提高了胚胎的桑椹率、囊胚率和孵化率；單獨添加葡萄糖的培養液，其桑椹率顯著升高，但囊胚率和孵化率的升高並不顯著，所以胚胎培養液中氨基酸和葡萄糖能協同促進胚胎發育。
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