嵌合基因 的英文怎麼說
中文拼音 [qiàngějīyīn]
嵌合基因
英文
chimeric gene-
The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment
目的本課題旨在探索一種新的str基因座復合擴增方法,我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺,建立一套新的法醫str基因座復合擴增體系,並按照美國dna分析方法技術工作組( thetechnologyworkinggroupondnaanalysismethods , twgdam )的指導方案進行法醫學實用性研究。The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a
其次,通過氨基酸序列和結構的比較,確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能;然後,應用重組dna技術,互換tm - 2 ~ 2基因和tm - 2基因的對應結構域,構建嵌合基因,獲得嵌合蛋白表達的轉化體,驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析,證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列,在iptg誘導下兩種嵌合分子都獲得了分泌表達,表達產物主要集中在細胞周質空間。Therefore, toreniafournieri is a very suitable model plant for studies of double fertilization in living material. we transferred the fusion gene ofgfp : mtn to toreniafournieri by leaf disc transformation mediated by agrobacterium and gain transgenic plant
我們將嵌合基因gfp : mtn通過葉盤法導入藍豬耳,探索了藍豬耳遺傳轉化的方法和轉基因苗的再生條件,建立了成熟的藍豬耳轉化系統。Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化Owing to the common expression of egf in various organs of mouse, it could be deduced that the role of egf was mainly autocrine and paracrine actions, and its endocrine role was also not excluded owing to its high expression abundance in submaxillary gland
由於egf在小鼠各器官中表達的普遍性,可以推測egf的作用以旁分泌和自分泌作用為主,但它在頜下博士學位論文「 tnegfalelittin 」嵌合基因構建及有關基因重組表達的研究等器官中的高表達表明其也具有內分泌作用。The tandem expression of the human salmon chimeric ct gene in e. coli
鮭降鈣素嵌合基因在大腸桿菌中的串聯表達Construction and identification of dna vaccine containing chimeric gene gag - gp120 of hiv
120嵌合基因核酸疫苗的構建與鑒定Construction of anther specific chimeric promoter and further establishment of transgenic artificial male sterile arabidopsis thaliana
花藥特異嵌合啟動子的構建及雄性不育轉基因擬南芥的獲得In summary, we constructed and characterized a bac library of silky, a unique chinese native breed of chicken. the library has high genome coverage ( 13 - fold ), chimerism ( 6 % ) and overlapped bacs, which made it a valuable resource to complete chicken physical map, study functional genes and construct bac contigs. we analyzed the whole genomic sequence and snps of tyrp1 and found that silky tyrp1 is different with other breeds both in microsatellite and transcription regulation site
中國農業人學博卜學位論文摘要本研究構建了中國特有雞種絲羽鳥骨雞基因組bac文庫並進行了文庫質量鑒定,它所具有的13倍高基因組覆蓋率、 6 %的嵌合率和部分重疊的克隆使其成為完善雞的基因組圖譜、研究基因功能和構建bac重疊群的優質資源。Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明: ( 1 )脂質體包裹外源基因轉染精子的方法,可將外源基因導入受精卵中,能夠獲得轉基因動物,並得到了較高的轉基因陽性率; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag
目的:以il - 12作為分子佐劑,與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子,將其克隆到真核表達質粒中,構建成嵌合dna疫苗,研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用,為尋求安全、有效、廉價的結核病新疫苗打下基礎。An organism consisting of two or more tissues of different genetic composition, produced as a result of mutation, grafting, or the mixture of cell populations from different zygotes
嵌合體由兩種或兩種以上有不同基因結構的組織所構成的有機體,由於突變、嫁接或來自不同合子的細胞群體相混合而產生。Because expressed in escherichia coli, ricin a chain is not degraded easily for its non - glycosylation. our experiments lay a foundation for the researches that rta conjugates a monoclonal antibody to synthesize immunotoxins and the immunotoxins are used for cancer - targeted therapy
蓖麻毒素a鏈在大腸桿菌中表達,因無糖基化修飾,克服了進入體內后被迅速降解的缺點,為進一步與單鏈抗體基因嵌合表達形成單鏈免疫毒素,對腫瘤細胞進行導向治療研究奠定基礎。Result. a method of multiplex pcr based on both chimeric and universal primers was established. a fluorescent quadriplex str system, including d1s1612, d9s2026, d20s161 and d6s477, was developed on the basis of the multiplex pcr
建立了dis1612 、 d952026 、 d205161 、 d65477str基因座熒光標一記嵌合引物復合擴增毛細管電泳激光自動分析體系,同時證明嵌合引物復合擴增方法與毛細管電泳激光自動分析方法相兼容。In order to express the recombinant peptide of both gp52 and pp150 oterminal peptides from human cytomegalovirus ( hcmv ), which seem to show good antigenicity. recombinant dna technology was used to construct recombinant plasmid, which was transformed into the pichia pastoris to express the interesting peptide
為了表達人巨細胞病毒( humancytomegalovirus , hcmv )中抗原性較強的兩段蛋白片段? gp52c末端和pp150c末端的嵌合肽,用基因工程技術構建適于酵母表達系統的重組表達質粒。2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增強的ta29啟動子驅動克隆的coda基因,構建成花藥特異性嵌合基因表達盒;將此表達盒插入雙元載體p3301 ,構建成以ppt抗性基因為選擇標記,以gus為報告基因的植物表達載體。Also, these str loci were amplified by multiplex pcr using our primer set, but the universal primers were fluorescent - labeled. the products of the multiplex pcr were genotyped by abi prism 310 genetic analyzer
結果通過採用公共引物和基因座特異性嵌合引物的方法,建立了法醫str基因座嵌合引物復合擴增方法。It may arise naturally due to mutation in a cell of a developing embryo, producing a line of cells with the mutant gene, and hence different characteristics compared to surrounding cells
嵌合體可以由發育著的胚胎細胞變異而自然形成,這樣就產生了具有不同性狀的變異基因的細胞系。Furthermore, by inserting " anther box " element to the mutated area of two site - mutation promoters, another two promoters, ipmas and ipmal, were created. in order to study the chemical - inducible capacity of wild and modified pr - la promoters, a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am, and the chimeric genes were cloned into pbin ! 9 - based plant expression vector
為了檢測得到的啟動子驅動效率及誘導活性,將所得到的啟動子、定點突變啟動子和插入花藥盒的啟動子與gus基因連接,構建了6個植物表達載體,同時分別構建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表達載體。分享友人