擬桿菌素 的英文怎麼說
中文拼音 [nǐgǎnjūnsù]
擬桿菌素
英文
convexin-
The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中,導入農桿菌后,進行植物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種子后,對其進行了分子生物學的驗證及生理指標的檢驗。Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )
將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中,導入根瘤農桿菌gv3101后,由花浸泡法進行擬南芥遺傳轉化,轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素( 25mg )的ms培養基上篩選,獲得t ;代轉基因植株。
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