整合蛋白質 的英文怎麼說

中文拼音 [zhěnggědànbáizhí]
整合蛋白質 英文
integral protein
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 整合 : commensuration
  • 蛋白質 : [生物化學] protein; proteide （舊稱「朊」, 由多種氨基酸結合而成的高分子化合物）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Plant proteins can be combined to include all of the essential amino acids and form. a complete protein

    植物蛋白可以結合到包括所有的必需氨基酸，並形成一個完整的蛋白質。
  3. Hla - g1, which is a newly defined non - classical hla class i molecule, plays an important role in mediating immunotolerance and protecting embryo and even some kinds of tumors from nk cells attacking. the full - length coding sequences containing cdna of hla - g1 were cloned from placenta, monocytes and liver cancer tissue of chinese donors. sequence analysis reveals that it is a highly conserved human gene with only two amino acid mutation sites compared to foreign nationality. its truncated form was overexpressed in

    從中國人外周血單個核細胞胎盤組織和肝癌組織等樣品中克隆了包含完整hla - g1讀框的cdna與國外同行獲得的該基因及其蛋白質序列比較分析表明，該基因雖然有著細微的種族特異性，但高度保守並獲得了它的截斷型重組蛋白，根據蛋白一級結構和同源比較方法，模建了它及其與特異性受體kir2dl4形成復合體的空間結構模擬，預測了它們之間相互作用的特徵。
  4. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  5. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  6. The product may compose various organic acid, protein, vitamins such as matter, amino acid in beef, pig, flesh chicken, flesh duck body, promote every organ adjusting and improving an animal individual growth, raise feed digesting absorption rate, reduce feed costs, improves the quality of meat

    本產品可在肉牛、豬、肉雞、肉鴨體內分泌合成多種酶類物質、氨基酸等多種有機酸、蛋白質、維生素、促生長因子，調整和提高動物各器官功能，促進個體生長，提高飼料消化吸收率，降低飼料成本，提高肉的品質。
  7. Examples of combined, complete plant proteins are rice and beans, milk and wheat cereal, and corn and beans

    實例結合，完整的植物蛋白質是大米和豆類，奶和穀物小麥，玉米和豆類。
  8. It " s the first cdna code ( 6 - 4 ) photolyase found in low orgnism ( alga ), and this study is important for the reseach of 6 - 4 photolyase. the methods and analysis as bellows : 1. this est was analyzed by method of blast, and the conclusion suggests that the sequence probably be a partial cdna that can code one of protein family of photolyase / blue light photoreceptor

    利用3 』 race法擴增此cdna序列的3 』端部分，所得片段長度為2575bp ，含一個完整的開放讀框，長1800bp ，可編碼600個氨基酸；利用生物信息學法對此擴增序列進行分析，包括同源性分析、序列的讀框分析、蛋白質的保守性分析以及該蛋白的進化關系分析，預測出此序列編碼了d . salina的（ 6 - 4 ）光裂合酶。
  9. The chemical components of silkworm pupa crust were analyzed, and its microstructure was characterized by using scanning electron microscope. the existing realtion of among chitin 、 protein and inorganic salt in silkworm pupa crust has been observed. the results show that the major protion of silkworm pupa chitin is in pupa crust, and it accounts for about one forth of crust weight, the out surface of pupa crust is regular polygon net vein characteristics. chitin takes honeycomb shape in chitin - protein complex and conjugated with protein. the inner space of chitin - protein complex net was filled with inorganic salts. thus the theory basis was provided for working out the process route of isolation pupa chitin

    對桑蠶蛹皮的成分、結構進行了化學及掃描電鏡分析，確定其含有的主要成分及含甲殼素的數量，並對其中的甲殼素、蛋白質和無機鹽三者之間的存在方式進行了觀察.研究結果表明，蛹體中的甲殼素與灰分主要含在蛹皮中，甲殼素占整個蛹體成分的2 . 71 % ，占蛹皮重量的25 . 5 % ，蛹皮外表面呈規整的多邊形網狀結構，蛹皮中蛋白質與蜂窩狀的甲殼素相結合，呈層狀分佈，顆粒狀的無機鹽填充在甲殼素/蛋白質復合物構成的蜂窩狀的空隙中.這為制定提取蛹甲殼素的工藝路線提供了理論依據
  10. Moving from hebb ' s theory to sorting out the actual mechanics of this process, however, one again confronts the fact that the enzymes and proteins that strengthen or weaken synaptic connections during brain wiring must be synthesized from specific genes

    不過，從赫柏的理論到?清整個過程中的實際機制，會再度面臨一個事實，那就是在腦建立線路的過程中，那些會強化或削弱突觸連結的酵素與蛋白質，都必須經由特定的基因來合成。
  11. Integrins, a large family of receptors that attach cells to the matrix, exert an extraordinary control on cell adhesion, migration, proliferation and survival

    摘要細胞外基質受體整合蛋白全方位影響細胞的形態、運動、存活和增殖。
  12. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  13. Plates for thin - layer chromatography ( tlc ) with an attached layer of porous polymer monolith have been prepared and used for the separation of small molecules, peptides, and proteins

    制備了塗覆有整體多孔聚合塗層的薄層色譜（ tlc ）板，並將其用於小分子、肽段和蛋白質的分離。
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