有旋毛蟲的 的英文怎麼說
中文拼音 [yǒuxuánmáochóngde]
有旋毛蟲的
英文
trichinous-
Because of the difficulties on diagnosis and treatment, it is very important for the prevention of trichinosis. trichinella spiralis ( t. s ) has a complex life history and a variety of antigen components, so, despite of a lot of studies, no prevention measures specific for t. s has been found until now
旋毛蟲病的診斷和治療都比較困難，因而預防顯得尤為重要。由於旋毛蟲生活史的復雜性和抗原的多樣性，雖然近年來許多學者進行了多方面的研究，但迄今為止，尚未發現免疫預防旋毛蟲病的有效措施。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Ts87 gene fragment was picked out by screening adult t. s cdna library using sera of rabbit raised against soluble antigen of t. s and using sera of rabbit infected artificially with t. s. the ts87 fragment was ligated to vector pcdnas. l, which contains a cmv promoter / enhancer
本研究採用通過免疫篩庫得到的旋毛蟲抗原基因片段ts87與載體pcdna3 . 1連接。該載體含有人類巨細胞病毒（ cmv ）的高效啟動子和增強子，是一種外源基因在哺乳動物細胞內高效表達的理想載體。
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