末端酶 的英文怎麼說

中文拼音 [mòduān]
末端酶 英文
terminal enzyme
  • 末 : Ⅰ名詞1 （東西的梢;盡頭） tip; terminal; end 2 （非根本、非重要的事物） nonessentials; minor detai...
  • 端 : Ⅰ名詞1 （東西的頭） end; extremity 2 （事情的開頭） beginning 3 （門類; 方面） item; point 4 （原...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 末端 : end; terminus; terminatio; terminal; tail end; extremity末端棒 terminal bar; 末端朝上 endways
  1. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  2. Full - length or truncated cdna was subcloned into prokaryotic expression vector pet30a and expression induced in e. coli bl21 ( de3 ). no squalene synthase polypeptide of expected molecular mass was observed in e. coli containing the putative full - length squalene synthase cdna, however, overexpression in e. coli was achieved by truncating 30 hydrophobic amino acids at the carboxy terminus

    但在含有全長的鯊烯合酶cdna的大腸桿菌中並沒有觀察到預期大小的鯊烯合酶表達，而c末端截短30個疏水氨基酸的鯊烯合酶可在大腸桿菌中過量表達。
  3. Introduction telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telo - meric dna sequences using its rna as template. it can remedy the loss of te - lomere after cellular mitosis, maintain telomere length and stabilize chromosome

    前言端粒酶（ telomerase ）是一種核糖核蛋白逆轉錄酶，能以自身的rna為模板，從頭合成染色體末端的端粒dna ，彌補細胞分裂時端粒dna的丟失，維持端粒的長度並穩定染色體。
  4. Because lung cancer cells may have some special hormone ( heterologous hormone ), and antigen enzyme, the role of these substances in the operation of bone joints, a result of bone and joint swelling pain, often involving the tibia, recife, ulnar and radial and bone and joints, often terminal expansion toes were clubbed fingers, x - ray radiography examination showed periosteal proliferation

    由於肺癌細胞可產生某些特殊的內分泌激素（異源性激素） 、抗原和酶，這些物質運轉作用於骨關節部位，而致骨關節腫脹疼痛，常累及脛、腓、尺、橈等骨及關節，指趾末端往往膨大呈杵狀指， x線攝片檢查可見骨膜增生。
  5. In escherichia coli, arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway. here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog, and the effects of n - terminus on feedback inhibition and its quaternary structure, and the importance of the structural " d2 " symmetry to allosteric inhibition

    本博士論文工作以大腸桿菌k - 12來源的arog為研究對象，通過定點突變、反饋抑制實驗和酶學動力學參數的測定，深入地研究了arog的反饋抑制位點的特性，並對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用，以及蛋白質結構的「 d2 」對稱性對酶功能的重要性等進行了具體的研究。
  6. The generation of endostatin is catalyzed by proteolytic enzymes, that cleave peptide bonds within the protease - sensitive hinge region of the c - terminal domain

    特異性蛋白水解酶降解膠原c末端的蛋白酶敏感區，產生內皮抑素。
  7. A conservative motif, recognized by proteinases of potato virus y, was inserted between nib and ppiv, which will release functional ppiv from the fused protein after infection by potato virus y. then, plant expression vector pnpa was constructed by ligating the fusing gene and pbi121, which is knocked out gus gene

    以馬鈴薯y病毒的復制酶（ nib ）基因為模板，通過聚合酶鏈式反應獲得nib基因，並在nib基因末端保留了在病毒基因組中nib與外殼蛋白（ cp ）基因相銜接的保守序列。
  8. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  9. Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization

    端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。
  10. But polyadenylation in bacteria needs no specific consensus sequence or there is no such sequence signals found. the sites of polyadenylation of bacterial mrna are diverse, including the 3 ' ends of primary transcripts, the sites of endonucleolytic processing in the 3 ' untranslatd and intercistronic regions, and sites within the coding regions of mrna degradation products

    細菌mrna多聚腺苷酸化的位點多種多樣，包括初級轉錄產物的3 』末端， 3 』端非翻譯區和順反子間區的內切酶加工位點及mrna降解產物的編碼區內，其腺苷酸化相對無特異性、無選擇性。
  11. - acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography

    本文對來源於枯草芽孢桿菌（ bacillussubtilis ） 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟，得到sds - paeg電泳純，通過n末端氨基酸序列分析驗證酶蛋白的純度。
  12. For example, hexokinase catalyzes the transfer of a high - energy terminal phosphate group form atp to glucose to give glucose 6 - phosphate and adp

    例如：己糖激酶可以催化atp最末端的一個高能磷酸鍵轉移到葡萄糖分子上，形成葡萄糖- 6 -磷酸和adp 。
  13. Telomerase is at end of the telomere, it helps to keep the sequence of dna, to offset or postpone the continuously shortening of the telomere while the cell division takes place

    端粒酶位於端粒末端，作用是合成端粒dna序列，以抵消或延緩端粒隨細胞分裂的不斷縮短。
  14. Rnase p from e. coli is a ribonucleoprotein complex responsible for the 5 " maturation of trnas. the smallest substrate contains two important parts : ncca - 3 " terminal and rna helix area

    Rnasep是結構識別酶， m1rna底物至少包括兩個要件： 1游離ncca - 3末端； 2特異互補的雙鏈rna區域。
  15. As well as in eukaryocyte ( hepg2 and cos - 7 ), then detect their antigenity as a basis study and explore of the choice of immunogen for preventive and therapeutic vaccines of hepatitis b. methods : the gene fragments coding 152aa ( si ) and 124aa ( s2 ) of the carboxyl terminus of hbsag were amplified by pcr from plasmid pecob6 with a pair of primers containing different endonuclease sites and were cloned into multiple cloning sites of plasmid pbks ( + )

    為乙型肝炎的預防和治療性疫苗免疫原的選擇進行初步的研究和探討。方法：本研究利用聚合酶鏈反應（ pcr ） ，通過設計帶有不同酶切位點的一對引物，從質粒pecob6特異性擴增hbsag蛋白羧基末端152個氨基酸（ s1 ）和124個氨基酸（ s2 ）的基因片段，分別將二者克隆到質粒pbks （ + ）的多克隆位點，篩選重組克隆。
  16. This method has several strong points : ( 1 ) eliminating the possibility of ringing self of vector. ( 2 ) the inserting fragments ca n ' t ligate each other. ( 3 ) the translating rate with the partially filled in method is equal to phosphatase method

    末端半補齊技術的優點有： （ 1 ）徹底消除載體自環化的可能性； （ 2 ）消除了插入片段自身相互連接的可能性； （ 3 ）同常用的堿性磷酸酯酶法相比較，採用半補齊技術其連接產物的轉化率不受影響。
  17. However where the product is nonproteinaceous, of low molecular weight and at the end of a long multienzyme sequence it is extremely unlikely that gene transfer to another host will be feasible

    然而，如果產品是低分子量且處于長多酶序列末端的非蛋白質類產品，轉基因技術的應用可行性極小。
  18. The mouse fertilized egg is the most simple and natural mammalian model of cell cycle, there are few reports about the mechanism on the regulation of early development of mouse fertilized eggs especially the events of entry into m - phase. its the first time we report here that pkb exists and effects on g2 / m phase transition in mouse one - cell stage fertilized eggs

    148 - 411是激酶區（催化域） ，和其它絲蘇氨酸蛋白激酶有較高的同源性，其中有一個保守的thr ，它位於308 、 309或305位；從412位起為c末端尾區（調節域） ，除pkb -外都有一個保守的ser ，位於473或474位。
  19. According to the nucleotide sequence of selected antigen epitope, pcr primers supplemented with the ecor i and sal i sites were designed

    根據選出的抗原表位區的核酸序列設計pcr引物，並於引物末端添加ecor和sa11酶切位點。
  20. Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr

    從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。
分享友人
分享到 Line

分享到臉書