杜克氏法 的英文怎麼說
中文拼音 [dùkèshìfǎ]
杜克氏法
英文
duke methods- 杜 : Ⅰ名詞1. （杜梨, 即棠梨） birch-leaf pear2. （姓氏） a surname Ⅱ動詞（阻塞） shut out; stop; prevent
- 克 : 克i 動詞1 （能） can; be able to 2 （克服; 克制） restrain; control 3 （攻下據點; 戰勝） overcome...
- 氏 : 氏名詞1 （姓） family name; surname 2 （放在已婚婦女的姓后 通常在父姓前再加夫姓 作為稱呼） née : ...
- 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
- 杜克 : bill duke
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In order to form a chloroplast transformation system of d. salina, we have conducted some studies including its sensitivity to antibiotics, the activity of promoter, cloning of the chloroplast genes and construction of transformation vectors, so far a pilot transformation system of the d. salina chloroplast has been completed. methods : the sensitivity of d. salina to seven antibiotics or herbicide used commonly in gene engineering was studied and the biological activity of atpa promoter from c. reinhardtii chloroplast was tested by using enhanced green fluorescent protein ( egfp ) as a reporter. primers were designed in the conservative encoding regions according to the chloroplast genomes from four algae which have close genetic relationship with d. salina, and the sequences of 16s rrna, chll and chln of d. salina chloroplast were cloned and sequenced, respectively
方法:根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，在基因編碼區的高度保守區域設計引物，克隆了杜氏鹽藻葉綠體165識na基因、咖l基因和ch n基因，並分別以165識na基因和chln基因序列為同源片段，以cat和bar基因為篩選標記基因構建了三套杜氏鹽藻葉綠體轉化載體: 2鄭州大學2003年博士學位論文杜氏鹽藻（ d ~ iiellasalina ）葉綠體轉化研究pds165一eaf 、 ptn1269一bar和psp72一5一bar一3 ，用基因槍法轉化杜氏鹽藻，初步建立起杜氏鹽藻葉綠體轉化體系。
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