染色質組型 的英文怎麼說

中文拼音 [rǎnshǎizhíxíng]
染色質組型 英文
karyotype
  • : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
  • : 色名詞[口語] (顏色) colour
  • : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
  • : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
  • 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
  1. The origin of such large amounts of constitutive heterochromatin and their role in karyotype evolution and speciation remain a mystery.

    如此大量的結構異的來源及其在進化和物種形成中的任務仍是一個謎。
  2. Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly

    方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模,主要採用抗fos 、膠原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫織化學多重、免疫電鏡、 wga - hrp束路追蹤結合免疫織化學多重、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性及其與ast的關系、培養神經元和ast內鈣波的變化。
  3. Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased

    腦內gfap陽性結構也明顯增多,其分佈與fos陽性細胞分佈基本一致,表現為胞體肥大、突起粗長; ast緊密包繞在神經元周圍形成神經元- ast復合體( n - asc ) ;在mvz 、 pvn和son三重免疫切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ; ( 2 )免疫電鏡下son內星細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區( edas ) ,高滲刺激后數量明顯增多: ( 3 )將們個mp注入大鼠一側n卜、卜卜或孤束核( ws ) ,分別在延髓內臟帶( mvz ) 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ; ( 4 )高滲刺激使培養神經元和ast內鈣水平先升高后降低,最後維持在比高滲刺激前稍高的靜息鈣水平上。
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    公司還生產lbz - a自動紙碗外套機lbz - aii全自動紙碗中空外套機lbz - aiii半自動紙碗外套機lbz - b全自動紙餐盒機lbz - bii半自動紙餐盒機lbz - c全自動充填封蓋機mlcb930平壓壓痕切線機等設備。我公司生產的系列環保設備是世界環境織清除白,倡導環境保護的推廣產品。
  5. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂體轉試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用) ;鼠單克隆抗體p21waf , (即用) ; dab試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
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    本品適用於后鎖以及中性、乾性、油性等各種類使用,內含豐富的太陽花植物原漿修護成份,能自動重頭發的纖維織,加強燙后頭發的彈性和持久度,獨有的雙重活性蛋白修復配方滲透力強勁,能瞬間滋潤秀發,能讓頭發快速吸收,能有效降低頭發的受損度程度,深層修復發內層結構。
  7. Inspection of germplasm for cultured fishes - part 12 : method for the karyotype analysis

    養殖魚類種檢驗第12部分:分析
  8. ( 2 ) effects on mouse spleen of so2 challenge : we found significant apoptotic changes of mouse spleen through tem observation and dna electrophoresis analysis and flow cytometric analysis. we found condensed, marginating, half - moon like apoptotic lymphocytes both in white pulp and red pulp ; we found significant dna degradation with dna ladders from the dna electrophoresis analysis in the 168 mg / m3 so2 treated group ; we also found marked increase of apoptotic rate between 168 mg / m3 so2 treated group and control group from the flow cytometric analysis

    ( 2 )二氧化硫吸入可引起小鼠脾臟細胞出現明顯的凋亡改變,紅髓區和白髓區淋巴細胞出現核固縮,凝聚、邊集; dna凝膠電泳分析發現168mg m ~ 3二氧化硫出現典的dna梯形條帶;流式細胞分析也發現高劑量的小鼠脾細胞凋亡率增加,並且與對照相比有顯著性差異, p 0 . 05 。
  9. Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells

    方法:採用成年大鼠肺織無血清培養,運用[ ~ 3h ] -膽堿摻入法測定ps主要脂磷脂酰膽堿( pc )合成量; rt - pcr擴增檢測肺織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶( cct ) mrna含量;透射電子顯微鏡法觀察肺泡上皮細胞和ps系統超微結構的變化;免疫織化學檢測glu的受體nmdar1亞單位的表達;生化測定肺織乳酸脫氫酶( ldh )釋放量和肺織勻漿中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。
  10. This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr

    本研究首先從hcv基因中擴增出nssb基因,構建了nssb基因與報告分子egfp (增強熒光蛋白)基因的融合基因真核表達粒pegfpn30 ;通過含有不同g418濃度梯度的培養液培養hepgz細胞,確定了篩選用g4181作濃度為800pg ml ;利用脂體法將該重粒轉hepgz細胞,經過有限稀釋法和g4壓力選擇,應用熒光顯微鏡和rtpcr檢測,獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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