核素掃描 的英文怎麼說
中文拼音 [hésùsǎomiáo]
核素掃描
英文
nuclide scanning-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The fabrication parameters were preliminarily optimized. the morphology and composition of the samples of the diamond film for different b / c ratios was investigated by scanning electron micrograph ( sem ) and raman scattering spectroscopy ( raman ). the content of different levels of b dopant in the diamond film was tested by secondary ion mass spectrometry ( sims )
闡述了摻硼金剛石膜的制備工藝，研究了摻硼金剛石膜成核和生長的影響因素，初步優化了沉積摻硼金剛石膜工藝參數，同時對摻硼金剛石膜進行了掃描分析、拉曼分析、二次離子質譜分析和電阻率測試。In order to meet the requirements of nda measurement, the laboratory of technical research for nuclear safeguards of china institute of atomic energy ( ciae ), is being in charge of developing four sets of nda standards : one set of segmented gamma - ray scanner ( sgs ) uranium standard, one set of sgs plutonhim standard, one set of plutonium isotopic standard, and one set of active well coincidence counter ( awcc ) standard. in this dissertation some pivotal techniques on quality control in developing of nda nuclear standards above were emphatically studied
中國原子能科學研究院核保障技術重點實驗室研製分段y掃描裝置（ sgs ： segmentedgamma - rayscanner ）鈾工作標準樣品、 sgs鈈工作標準樣品、鈈同位素標準樣品和有源中子井型符合計數裝置（ awcc ： activewellcoincidencecounter ）鈾工作標準樣品來滿足核材料nda測量的要求。本論文重點對上述nda核標準樣品研製過程中質量控制的一些關鍵技術進行了研究。Determination of emodin in kangyanning granules by tlc - scanning
雙波長薄層掃描法測定梔核清肝膠囊中大黃素含量This nuclear medicine bone scan reveals multiple areas of increased uptake, which are the darker foci, such as in the vertebral column representing metastases
核素掃描顯示多個病灶，脊柱周圍顏色較深的病灶代表轉移性病變。The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。A little ferrite and cementite, through the analysis of the strengther and ductiler, we draw the conclusion that this is very important in reality, observing the graphite nodule in sem and tem, there are many spheroiding element and anti - spheroiding elements in the core and edge of graphite nodule, they form the oxides sulphide and nitride. we draw the conclusion that they may be the core of graphite nodule
在掃描電鏡（ sem ）與透射電鏡（ tem ）上觀察石墨球，與傳統石墨球相比低碳球鐵的石墨球呈細小點狀分佈，球墨中心聚集有較多的球化元素而在邊緣處分佈有反球化元素。石墨球中心存在有氧化摘要一物、硫化物以及氮化物等組成的復雜的化合物，經分析認為它們是球狀石墨形核的有效核心。分享友人