核酸數據庫 的英文怎麼說

中文拼音 [hésuānshǔjùkù]
核酸數據庫 英文
nucleic acid
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 數 : 數副詞（屢次） frequently; repeatedly
  • 據 : 據Ⅰ動詞1 （占據） occupy; seize 2 （憑借; 依靠） rely on; depend on Ⅱ介詞（按照; 依據） according...
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  • 數據庫 : data bank; data base; data pool; data library數據庫管理系統 data base management system; 數據庫計...
  • 數據 : data; record; information
  1. Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end. then resuspended the lyophilized probes in ph = 9, 0

    寡核苷酸探針的制備根據genbank數據庫新近公布的hla - dqa1等位基因序列，設計四組共16條特異性寡核苷酸分型探針，並在各5 』端做氨基修飾。
  2. The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned

    從陽性克隆子中提取克隆片段，經序列測定分析，結果表明，克隆片段的cdna大小為816bp ，編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank ， embl ， ddbj ， pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索，結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ；蛋白質氨基酸序列的同源率為90 95 ，表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4
  3. The clsp protein shared roughly 25 - 40 % identity and 40 - 55 % similarity to complement subcomponent clr, cls, mannose - associated serine protease - 1 ( masp - 1 ), masp - 2, and masp - 3

    通過在genba川k / embl數據庫中對clsp序列進行同源性搜索和分析，我們發現其核酸和氨基酸序列和人clr 、 cis 、 maspz 、 maspz 、 masp3等有高度同源性。
  4. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  5. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  6. In this paper several methods of developing microsatellite primers in plants were summarized : to search the database and find the primers which were delivered ; to transfer them among the different species ; to establish genomic library ; to develop ssr primer based on moleculars markers

    摘要綜述了開發植物微衛星引物的幾種方法：搜尋核酸數據庫和尋找已存在的微衛星引物；不同物種間共用；建立基因組文庫；利用分子標記技術發展ssr引物。
  7. Three special bands as pcr products, obtained by using primer no. l, are reclaimed, and then used to cloning and sequencing. through homologous comparison with data from international nucleotide databank, the band ' s functions have been simply analyzed

    對1號簡並引物擴增出的3條特異性條帶進行了回收、克隆和測序；與國際核苷酸數據庫進行了同源性比較，對特異性片段的功能進行初步的分析和確定。
  8. Sds - page and activity staining analysis revealed that all of clones 2, 5 and 9 exhibited a single active band with 83 - kd in size, whereas clone 3 exhibited four different bands with 64 kd, 70kd, 76kd and 83 kd after construcing a series of deleted subclones from the plasmid prepared from clone - 5, a complete nucleotide sequences comprising of 3, 205 bps wes determined. one open reading frame ( orf ) was found in this sequence, which was comprised of 2, 661 bps and could encode a polypeptide with 887 amino acid residues

    利用基因分析軟體genetyx對clone - 5質粒的核苷酸序列進行分析，並搜索日本dna數據庫（ dnadatabankofjapan ，簡稱ddbj ） ，推斷clone - 5質粒的幾丁質酶基因含有一個2 ， 261bp的開放閱讀框（ orf ） ，編碼一條887個氨基酸的幾丁質酶蛋白； orf上游有一個啟動子。
  9. Furthermore, suppression subtractive hybridization ( ssh ) was employed for the isolation of cdna fragments for euonymus japonicus " zhuzi " differentially expressed genes, and forward suppression subtractive cdna library of cold - regulated genes was constructed. the seedlings of euonymus japonicus " zhuzi " treated with low temperature were as tester and untreated seedlings as driver. subtractive cdna library was differentially screened through cdna macroarray, six hundreds and four cdna clones were identified as cold specifically induced or highly expressed

    （ 5 ）應用抑制差減雜交（ suppressionsubtractivehybridization ， ssh ）方法，構建冷誘導表達的正向抑制差減cdna文庫，低溫處理的幼苗為tester ，常溫處理為driver ，通過cdna微陣列差異篩選cdna文庫，得到604個低溫誘導或表達增強的候選克隆，對其中的84克隆進行dna測序，去除冗餘的cdna ，在genbank中進行核酸和蛋白質同源性的比較和功能分析，共有36個單一序列，其中12個cdna在genbank數據庫沒有同源的序列。
  10. Each of the human mrna sequences was blasted against mouse mrna sequences and the top hit was blasted back to the original human mrna database. if the top human mrna hit is the same as the original human mrna, we assumed an orthologous relationship between the human and mouse genes. to minimize the redundancy, only human mrna in genbank refseq section was used to establish any human - mouse ortholog relationships

    每個mrna用blastn程序與genbank數據庫中的小鼠核酸序列進行比對，找出與其匹配程度最高的mrna序列，將該序列重新用blastn程序與數據庫中人的核酸序列比對，如果其中匹配程度最高的序列與最初輸入的人mrna序列一致，則將小鼠中這條序列的編碼基因作為人類該基因的同源基因。
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