核酸結合蛋白 的英文怎麼說
中文拼音 [hésuānjiēgědànbái]
核酸結合蛋白
英文
nucleic acid binding protein- 核 : 核構詞成分。
- 酸 : 酸構詞成分。
- 結 : 結動詞(長出果實或種子) bear (fruit); form (seed)
- 合 : 合量詞(容量單位) ge, a unit of dry measure for grain (=1 decilitre)
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
- 結合 : 1 (發生密切聯系; 聯合) combine; unite; integrate; link; binding; coalition; cohesion; connectio...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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At rest, the heterodimeric rel / nf - k b complex is located in the cytoplasm bound to an inhibitory factor, i k b. upon stimulation, i k b is phosphorylated and degraded, free nf - k b then translocates into the nucleus where it binds to k b site to regulate transcription
靜息時, re皿4bh聚體與抑制蛋白kbs結合,在細胞漿中保持無活性。受到刺激, ikb分子迅速磷酸化並降解,釋放nf兒b轉位入核,通過與kb位點結合調節轉錄。Flavoprotein a conjugated protein in which a flavin ( fad or fmn ) is the prosthetic group joined to a protein component
黃素蛋白:一種以黃素核苷酸(黃素腺嘌呤二核苷酸fad或黃素單核苷酸fmn )為輔基的結合蛋白。Blast result showed the fragment containing the opuaa and its 5 " upsteam sequence was obtained
通過blast比較,表明獲得完整的atp結合蛋白基因orf框序列及其上游的核苷酸序列。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。Results show that vp37 protein can bind single strand nucleic acid cooperatively and nonspecificallty, and the vp37 - ssrna complex was stable at high salt concentrations, suggesting vp37 is a possible mp. vp37 is the only protein characterized so far showing rna - binding ability in genus fabavirus
為了驗證vp37是否具有核酸結合能力,我們利用6his - vp37蛋白進行了核酸結合實驗,結果表明vp37是一種能非特異性結合單鏈核酸的蛋白;其在結合核酸時具有協同性; na ~ +的變化對其核酸結合能力影響較小。Studies revealed that p - catenin dissociated from ccc and translocated into free catenin pool in cytosol after it has been phosphorylated at tyrosine or serine residues, and in this situation, the ccc has been disrupted and cell adhesion function disturbed. a large amount of the free p - catenins in the cytosol can be degraded by the tumor suppressor apc, and the remains translocate into nucleus and bind to transcriptional factor tcf / lef in the nucleus and then promote cell proliferation related gene or anti - apoptosis gene transcription
當-連環蛋白酪氨酸或絲氨酸殘基磷酸化后,就與ajs發生解離而游離到細胞漿中,此時細胞的粘附功能也發生障礙,游離到胞漿中的-連環蛋白,一部分被抑癌因子apc降解,一部分則轉移到細胞核內,與核內的轉錄因子tcf lef結合,啟動與細胞增殖有關的基因轉錄。At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中,利用隨機12肽庫對抗豬瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的單抗a11進行表位篩選,經過四輪篩選以後,隨機挑取11個克隆作競爭- elisa檢測,結果表明,所挑11個克隆中,有9個克隆能對me2蛋白和a11反應產生抑制作用,抑制率最高可達64 ; dna測序以後經過dnastar軟體分析,發現它們的核心序列為anwralsl ,該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性;夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別;人工合成含核心序列的多肽經間接elisa試驗證實,也能被a11識別。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列,結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性,設計了echistatin編碼基因,體外人工合成編碼基因dna片段,通過適當的限制性內切酶位點插入表達載體pbv220 ,分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆;進一步通過pcr技術構建echistatin的融合表達基因克隆。Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained
再次,根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物,與atp結合蛋白的上游簡並引物組合,經pcr擴增獲得2 . 1kb的條帶,測序后通過blast比較,結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。It has been shown that domain ia is responsible for cell recognition, domain ii is to be involved in translocation of the toxin across membranes, and domain hi catalyzes the adp - ribosylation of elongation factor2, which arrests protein synthesis and results in cell death
人組蛋白h3是堿性核蛋白,富含精氨酸,在生理條件下, h3的精氨酸帶正電荷,而dna的磷酸基團帶負電荷,所以組蛋白和dna分子主要依靠靜電引力相結合。The surface - modified magnetic nanoparticles have been widely used in biological and biomedical areas such as immobilization of biomolecules ; separation of metal ions, cells, proteins, dna ; immunoassay, biochemical analysis and magnetic resonance imaging ( mri ) probes
其中的殼結構既具有生物適應性,又具有可鍵合生物分子如細胞、蛋白質、酶、抗體和核酸的活性基團,而核具有磁性特性。Ebv gp350 and c3d bind selectively to the same site on the cr2, epitopes on scr - 1 and scr - 2 are necessary for the binding. as soon as c3d binds to scr - 1 and scr - 2, cr2 may cross - link - 8 - to memberane ig and form a " co - capping " structure, moreover, activate b lymphocyte through cd19, a component of cd19 / cd21 / cd81 / leul3 complex, signaling and / or nucleolin tyrosine phosphorylation independent on cd 19 signaling pathway
配體與scr l和scr 2結合后, crz與細胞膜免疫球蛋白( inlg )形成「共帽」 kofthofcccptor )結構,並經過d19c 21兒81肚u13復合體中d19的信號轉導以及不依賴于cd途徑的核仁素( nucleolin )酪氨酸磷酸化激活b細胞。For these goals, the fo llowing jobs have been done and some results have been obtained. 1 according to the vitreoscilla hemoglobin ( vhb ) amino acid sequence and plajnt preference codon usage, vgbm gene was designed and synthesized by annealing 22 synthetic fragments respectively, the modified vgbm gene of full length of 450 base pairs was synthesized. 2 the transformants were obtained after pbv221svhb was introduced into e. coli
為此本論文作了以下工作並得出了一些結論: 1根據透明顫菌血紅蛋白( vhb )的氨基酸序列,選用植物偏愛密碼子,對透明顫菌血紅蛋白基因( vgb )進行優化改造,設計併合成了22條寡核苷酸短片段,人工合成了改造的全長450bp的透明顫菌血紅蛋白( vhb )基因( vgbmWhen copper exceeds the cellular needs, it is toxic through the production of highly reactive hydroxyl radicals, that have deleterious effects on cellular components including destabilization of plasma membranes and lysosomal membranes
但是也具有毒性,銅可以與一些脂質,蛋白質和核酸等物質高親和性地結合,並催化其氧化,所以維持銅濃度在一定的正常范圍很重要。According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat, we designed primers and got the structure gene successfully. by 3 ' - race method combined with nested pcr, the 3 ' - end nuclear acid sequence was also obtained ; in additon, for the 5 ' - end sequence, we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer, and till now, partial 5 ' - end sequence has been amplified as well
本研究根據先前分離純化所得天然tb22kda蛋白經maldi - tof - ms (質譜法)測得的氨基酸序列和文獻報道的過敏蛋白核苷酸序列設計引物,擴增克隆了該過敏蛋白結構基因的編碼序列;根據測得的序列設計特異性引物,並利用3 』 - race方法結合巢式pcr擴增得到基因的3 』末端;依據同源性比較的結果選用一段保守序列為5 』引物,並根據結構基因內部序列設計3 』特異性引物,進一步獲得了該基因5 』端的部分序列。Pharmacogenomics combines traditional pharmaceutical sciences such as biochemistry with annotated knowledge of genes, proteins, and single nucleotide polymorphisms
藥物基因組學結合了傳統藥學理論(如生物化學)和已經定義了的基因、蛋白質和單核苷酸多態性等各類學科的研究成果。The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs
建立cdna表達文庫在一次永久保存基因資源的同時,可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因,進而克隆和表達這些基因,對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律,對揭示其抗藥性分子機理,搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制,對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。So it is important to reveal the mechanism of uptake and release of copper in hepatocyte, we aimed to purify a novel copper binding protein from rat liver lysosomal fraction. researches show that copper binding proteins have conserved amino acid sequences - mxcxxc ( x is any amino acid ), can bind with copper. for example atp7b has five mxcxxc sequence, and it was determined 1 mol atp7b can interact 5 mol of copper ions
Irene等人的研究表明鋼結合蛋白具有高度同源性的鋼結合氨基酸序列,從酵母等低等動物到真核細胞n末端都有mxcxxcx代表任意氨基酸廠是鋼結合的區域,例如ai 」 ob的n末端就有5個mxc狀c結構,實驗也證明一摩爾的蛋白質可與5摩爾的硫酸銅結合。Eng. ) introduction, amino acids, protein ' s primary structure and its biological function, protein secondary, tertiary, quaternary structure, carbohydrate, lipid, membrane, nucleotides and nucleic acids, structures of nucleic acids
中)簡介,胺基酸,蛋白質的一級結構及生物功能,蛋白質的二、三、四級結構,碳水化合物、醣類,脂質,細胞膜、與膜的運輸,核?酸與核酸,核酸的結構。In order to know whether vp37 has the ability to combine nucleic acid, which was thought as the basic characteristic of plant virus mp, gel - retardation and uv - crosslinking assays were employed
病毒的移動蛋白具有結合核酸的能力,包括移動機制與管狀結構和病毒粒子相關的移動蛋白。分享友人