核酸聚合酶 的英文怎麼說
中文拼音 [hésuānjùgě]
核酸聚合酶
英文
nucleic acid polymerase- 核 : 核構詞成分。
- 酸 : 酸構詞成分。
- 聚 : 動詞(聚集; 聚積) assemble; gather; get together
- 合 : 合量詞(容量單位) ge, a unit of dry measure for grain (=1 decilitre)
- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
- 聚合 : 1 (聚集到一起) get together2 [化學] (單體結合成高分子化合物) polymerization; polymerize 3 [生...
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In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
應用反轉錄-聚合酶鏈反應( rt - pcr )獲得兩株新城疫病毒( ndv )血凝素-神經氨酸酶( hn )基因cdna ,然後定向插入真核表達質粒pcdna3中,構建了含hn基因的重組質粒。As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template ' s sequence is revealed
當聚合酶將一個核苷酸加在新互補鏈的起始引子之後,或接合酶認定某段核苷酸鏈與原始模版配對,就可利用這些反應來得出原始模版的序列。The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage
此外,本文還選用咖啡酸純品來突破切割體系與識別體系(用氨基臂修飾的寡聚脫氧核苷酸)的連接,並用高效液相色譜法分析其偶聯產物,為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。The reproductive organ blister measles therefore recur, is because blister measles virus deep hiding in ganglion " the establishment gram kj medicinal preparation " series medicineis one kind of structure medicine, it ordinary disease - resistant poisonous medicine composition member is younger than several hundred times, can seep the nerve and the ganglion from the extroversion which suffers injury, is same along with it to sponge absoring water, layer upon layer strips the adsorption in the ganglion the crazy duplication viral body, the destruction virus s nucleotide duplication enzyme, causes it to be separated from the nerve is separated from the virus can massive gathering in the reproductive organ hypodermic, by now again coordinated the establishment gram venereal diseases kj medicinal preparation formidable anti - virus function, comprehensively struck kills the virus, caused the virus not to hide the place, thus achieved thoroughly permanently cured goal
安立克kj劑"系列藥物是一種微分子結構的藥物,它比普通抗病毒藥物的組成分子小幾百倍,能夠從外向內滲透進受損的神經和神經節,隨之就向海綿吸水一樣,層層剝離吸附在神經節里瘋狂復制的病毒體,破壞病毒的核苷酸復制酶,使其脫離神經.脫離出來的病毒會大量的聚集在生殖器皮下,這時再配合安立克性病kj劑強大的抗病毒作用,全面擊殺病毒,使病毒無藏身之地,從而達到徹底根治的目的Standard guide for detection of nucleic acids of the mycobacterium tuberculosis complex and other pathogenic mycobacteria by the polymerase chain reaction technique
用聚合酶鏈式反應方法探測結核分支桿菌復合物和其它病原分支桿菌的核酸的標準指南Part one the studies of piezoelectric biosensors and detection of staphylococcal enterotoxins chapter one the progresses in piezoelectric biosensors and detection of staphylococcal enterotoxins section one the progresses in piezoelectric biosensors in this section, a review is reported with 69 references about the piezoelectric biosensors, including the principle and constitutes of pebs, the classification of pebs, surface modification technique in pebs and the application of pebs in immunoassay, detection of the gene and microorganism, coupling with other techniques, moreover, the development of pebs was involved
引用參考文獻69篇。第二節金黃色葡萄球菌及其毒素檢測研究進展該部分內容主要介紹了在葡萄球菌分類與分型、金黃色葡萄球菌毒素學研究以及金黃色葡萄球菌及其毒素檢測等方面的研究進展,重點對金黃色葡萄球菌及其毒素檢測方法:生物學檢測方法、免疫血清學方法、聚合酶鏈反應技術、核酸雜交技術、生物傳感器技術和超抗原技術在近幾年的研究進展做了評述,引用文獻69篇。火nxia即。In this study, it has been put forward that taking reactive nanometer magnetic fe304 particles as magnetic nucleus, and the copolymer of styrene ( st ) ? crylic acid ( aa ) as macromolecular shell, we could synthesize, magnetic polymer composite microspheres containing carboxyl groups on their surface, then microspheres are activated by thionylchloride, the surface of such magnetic composite microspheres thus produced had reactive acid chloride groups which then react with the free amino groups of the free soluble enzymes to give peptide bonds ( ? o ? h ?,
本研究首次提出了以納米級磁性fe _ 3o _ 4粒子為核心,苯乙烯( st ) ?丙烯酸( aa )共聚物為高分子殼層,合成了表面帶羧基的磁性高分子復合微球,然後將這種微球用二氯亞碸進行活化處理,在其表面形成了反應性酰氯基團,該基團可以與游離酶的氨基形成肽鍵,從而將游離酶固定化。Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected
Rapd (隨機擴增多態性dna )技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術,它建立於聚合酶鏈式反應( pcr )技術基礎之上,利用隨機合成的寡聚核苷酸序列為引物(一般為10個bp ) ,分別與dna的兩條單鏈結合,在dna聚合酶的作用下,對基因組的特定區域進行pcr擴增,其電泳結果為不同大小和數目的dna譜帶即rapd圖譜,可反映基因組相應區域的dna多態性。The deduced amino acid sequence revealed that the isolates from guangdong province possibly were the primary source of afvs of mainland china. the central china area, such as henan province, may be a secondary source to the north and west of china. atvs changed by and by in the south district of mainland china, just like nan jing and shanghai
本實驗將在中國大陸1994年至2001年期間,不同地區、不同年代和不同宿主中分離到的h9n2亞型禽流感病毒株,應用逆轉錄聚合酶鏈反應rt - pcr擴增出該亞型病毒的血凝素基因ha ,經測序,獲得的30株中國大陸h9n2亞型禽流感病毒分離株的核苷酸序列。A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白( f )基因裂解位點的核苷酸序列與其毒力的相關規律,分別設計合成了四條寡核苷酸引物,建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應( rt - pcr )技術。Standard guide for detection of nucleic acid sequences of the human immunodeficiency virus hiv - 1 by the polymerase chain reaction technique
用聚合酶鏈反應技術探測人體免疫缺乏病毒hiv - 1的核酸類的標準指南And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭,在接頭序列和測定的f片段近tn5的序列上,設計了2對染色體爬行用的pcr引物,從b8菌株中提取基因組dna , bamhi酶切,與染色體爬行接頭連接,依次用2對引物進行pcr ,擴增出239bp產物,經克隆、測序,發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列,其餘則為f片段728bp序列的一部分,為進一步進行染色體爬行,克隆和測定整個b和f基因,揭示陽菌株的拮抗分子機制提供了技術資料貯備。To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法:根據端粒酶htert基因1573 ? 1591位的核酸序列,構建帶t7啟動子的部分雙鏈dna模板,用t7rna聚合酶體外合成短鏈shrna 。分享友人