桿疫病 的英文怎麼說

中文拼音 [gǎnyìbìng]
桿疫病 英文
pole blight
  • 桿 : 桿名詞（桿子） pole; staff
  • 疫 : 名詞（瘟疫） epidemic disease; pestilence
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 疫病 : blight; pestilence; loemia; loimia; epidemic disease; [植物學] phytophthora disease
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin

    在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. In this study, the meaningful results have been achieved, which is the important basic work to research the pili subunit vaccine of avian e. coli, and detect pila gene by nucleric acid probe. moreover, it is significant to research molecular epidemiology, to diagnose, to prevent and treat avian colibacillosis

    本研究為雞源致病性大腸桿菌菌毛基因工程疫苗的研究，制備核酸探針檢測pila基因提供了重要材料，對雞大腸桿菌病的分子流行病學研究、診斷和防治研究具有重要意義。
  5. These unseen bugs can be friends such as the bifidobacteria that we find in yoghurt or they can be our deadly foes such as yersinia pestis, the bacterium that caused the black death that decimated europe in the middle ages

    這些看不見的細菌可以是人類的朋友，例如乳酪中的雙歧桿菌。它們也可以是致命的敵人，好像引起黑死病、在中世紀時期奪去歐洲大量人命的鼠疫耶爾森氏菌。
  6. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  7. Type b component for 5 - in - 1 combination vaccine and additional hib and hepatitis b virus components for 6 - in - 1 combination vaccine. the additional components of 5 - in - 1 or 6 - in - 1 combination vaccines are hib and hbv

    除白喉破傷風百日咳及小兒麻痹癥外，五合一混合疫苗提供對乙型流感嗜血桿菌的保護而六合一混合疫苗則額外提供對乙型流感嗜血桿菌及乙型肝炎病毒的保護。
  8. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  9. Acellular as well as inactivated polioviruses types i, ii iii. the old dtwp vaccine is also a combination vaccine with similar components for diphtheria and tetanus but contains the " whole - cell " component for pertussis and does not contain the poliovirus component

    新疫苗的白喉及破傷風類毒素成份與以往所用的三合一白喉破傷風百日咳混合疫苗dtwp相若，而以往所用疫苗的百日咳桿菌為全細胞型及不含小兒麻痹病毒。
  10. Colibacollosis serotype k. 99 positive sera in ducks and duck plague positive sera were tested, whereas, the results of detection to r. a. sera were positive. the sensitivity of indirect elisa were 50 to 100 times ( serotype 1 ), ( 2 ) 25 to 100 times ( serotype 2 ), ( 3 ) 12 to 100 times ( serotype 4 ) and 25 to 200 times ( serotype 5 ) th an micro - agglutination test

    包被鴨疫里默氏桿菌抗原時，對鴨抗5 ： a多殺性巴氏桿菌陽性血清、鴨抗型鴨病毒性肝炎陽性血清、鴨抗鴨源大腸桿菌k _ （ 88 ）陽性血清、鴨抗鴨源腸炎沙門氏菌陽性血清、鴨抗鴨源大腸桿菌k _ （ 99 ）陽性血清、鴨抗鴨瘟陽性血清檢測結果呈陰性。
  11. The plague is a kind of when cause by the plague bacili spirited contagion, basically pass the flea on mice body, the plague bacili in blood of inspiratory disease rat, bite a person to cause disease again, form " rat one flea one person " transmission mode, sick also rat is gnawed bite a person to have transmission with the channel such as food

    鼠疫是由鼠疫桿菌引起的一種烈性傳染病，主要通過老鼠身上的跳蚤，吸入病鼠血液中的鼠疫桿菌，再次叮咬人致病，形成「鼠一蚤一人」的傳播模式，也有病鼠啃咬人用食品等渠道進行傳播。
  12. The high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm did n ' t react with the antibodies against pasteurellosis, streptococcosis, colibacillosis, chlamydiosis, hcv, ppv, brucellosis, prv and foot - mouth disease. the diaphragm has good sensitivity and could detect some salmonella - positive test serum which has been diluted to 1 : 2048. stored at 4 for at least 6 months or at 10 - 25 " c for 4 months, the sensitivity and specif icity of the diaphragm did n ' t change, so it has good stability

    本研究制備的診斷膜片特異性強：不與豬衣原體病、豬口蹄疫病、豬大腸桿菌病、豬布氏桿菌病、豬瘟、豬偽狂犬病、豬細小病毒病、豬巴氏桿菌病、豬鏈球菌病的陽性血清發生交叉反應；診斷膜片具有良好的敏感性，能夠檢測到1 ： 2048稀釋的動物試驗陽性血清；膜片的保存期長，在10 25可保存4個月、 4條件下至少可保存6個月其靈敏度不變。
  13. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。
  14. They are especially vulnerable to foodborne illness because their immune systems are not fully developed and their stomachs also produce less acid. for example, infants and young children are more likely to develop complications arising from infection with escherichia coli 0157 : h7

    嬰幼兒的免疫系統發育尚未成熟，胃酸分泌也較少，所以也較容易患上經食物傳播的疾病，例如當他們受o157 : h7型大腸桿菌感染時，出現並發癥的機會較高。
  15. The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna. the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene

    應用重組痘病毒表達的meq制備的單抗23b46對重組桿狀病毒感染的sfg細胞及其裂解物分別進行間接免疫熒光試驗、 westemblot和免疫沉澱試驗的檢測。
  16. During the past decade, escherichia coli o157 : h7 has evolved from a clinical novelty to a global public - health concern. the strains of e. coli o157 : h7 was isolated from sporadic diarrheal patient in 1980s, outbreaks caused by e. coli o157 : h7 have been observed in 1999 and 2000 in some areas

    我國1987年首次從腹瀉病患者糞便標本分離到大腸桿菌o157 ： h7 ， 1999年在某些地區發生了大腸桿菌o157 ： h7引起的暴發， 2000年疫區有擴大的趨勢。
  17. A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing

    提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。
  18. A - l was infected on monolayer sffi ther the third plaque purification to reproduce in quantity and named it a - 2. after a - 2 was infectal on sffi monolayer cell 4d harvested the cpe cell. the analysis results with sds - page and dot - elisa showed that prv vp7 gene was - - o - - abstract expressed in baculovirus

    接種a - 2代病毒于sf9單層細胞上， 4d后收獲病變細胞，通過sds -聚丙烯酰胺凝膠電泳（ sds - page ）和免疫酶斑點技術（ dot - elisa ）分析，表明prv - vp7基因在桿狀病毒表達系統中得到了表達。
  19. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  20. Infectious bursal disease virus has been a great concern for the poultry industry for a long time, particularly for the past decade when its " re - emergence " in variant or highly virulent forms. in this study, two sets of primers ( pta and pts, ibda and ibds ), flanking the hyper - variable region of vp2 gene, were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr. both of these assays can amplify all of 12 reference strains which including pathotypes cibdv, vvibdv and vibdv, but not the 5 negative reference pathogens of chicken

    結果12個參考毒株均能擴增出約679bp的目的片段，而陰性對照的常見5種雞病病原：雞新城疫病毒（ ndv ） 、雞傳染性支氣管炎病毒（ ibv ） 、雞傳染性貧血病毒（ caiv ） 、大腸桿菌和多殺性巴氏桿菌均沒有擴增到任何片段；應用建立的技術對疑似ibd的34份臨床病料進行檢測，並同時在基礎rt - pcr擴增的片段內設計另一對引物進行nested - pcr 。
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