標記抗體 的英文怎麼說
中文拼音 [biāojìkàngtǐ]
標記抗體
英文
labelled antibody- 標 : Ⅰ名詞1 [書面語] (樹梢) treetop; the tip of a tree2 (枝節或表面) symptom; outside appearance; ...
- 記 : Ⅰ動詞1 (把印象保持在腦子里) remember; bear in mind; commit to memory 2 (記錄; 記載;登記) writ...
- 抗 : Ⅰ動詞1 (抵抗; 抵擋) resist; combat; fight 2 (拒絕; 抗拒) refuse; defy 3 (對等) contend with...
- 體 : 體構詞成分。
- 標記 : (標志; 記號; 貨物標記) tab; sign; stamp; peg; label; mark; flag; blip; notation; fleck; track; ...
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The data above showed that algae phycobiliprotein fluorescent probes not only facilitate the study to pea actins but also provide a doable method to label other plant proteins. it has a good foreground for algae fluorescence probe to be used in the immunity fluorescent detection of plant cells. the gene clon
對豌豆肌動蛋白藻熒光探針的初步研究,不僅為豌豆肌動蛋白的免疫熒光檢測提供了便利條件,而且通過改變抗體,則可以將藻熒光探針用於其他植物蛋白的標記,藻熒光探針優越的熒光特性使其在植物材料的免疫熒光檢測中具有廣闊的應用前景。The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label
酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用,其靈敏度取決于標記酶的高度純化和高轉化率。Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test, rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test
試驗三:間接elisa檢測的抗體活性。用過氧化物酶標記的兔抗狐igg和雞igy抗體進行間接elisa測定獲得的樣品的活性。The prepared igg immunoprobe was found to have a sensitively nernst response to igg because of the antigen - antibody reaction. the change of potential from beginning to end ( the potentimetric difference, a
用戊二醛交聯法在鉑電極、銀電極上固定igg抗體,制備了可重復使用的電位型無標記igg免疫探針。In this study, genetic diversity of various strains of spirulina platensis is analyzed with rapd molecular marker and antibiotics marker, the results are compared with their morphologic characters, aimed at constructing a more reasonable classification criterion and knowing more about the genetic background of spirulina. additionally, it is found that under the cultivated condition the linear filament can retransite to the normal curved one which is similar to original spiral filament
本論文利用rapd分子標記及抗生素抗性標記分析了不同鈍頂螺旋藻品系的遺傳多態性,並與其形態學特徵進行了比較,以期建立更合理的螺旋藻分類方法及加深對螺旋藻遺傳背景的了解;同時,發現在養殖環境下變直藻絲體可以回變為正常螺旋形態的現象。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團,以tarma標記探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目標,設計、篩選出特異性引物和探針,優化實驗參數,建立了轉基因植物通用性熒光pcr定性檢測方法體系。We constructed a binary vector carrying a modified human afgf gene and a mana gene that allows the use of pmi selection system, an antibiotic and herbicide - free system, during the transformation procedure
我們構建的雙元表達載體帶有一個經過改構的人類酸性成纖維細胞生長因子基因( afgf ) ,載體上的選擇標記基因為mana ,它使得轉化過程中可以使用pmi這種不含抗生素及除草劑的選擇系統。The capture of the labeled antibody ? analyte complex for signal generation can be achieved with the antibody immobilized in a defined area of nitrocellulose membrane as mentioned
對產生信號的標記抗體抗原復合物的捕獲,是用抗體固定在已經提到的硝酸纖維素的特定區域內來達到的。Security, too, is a key issue for switching the traditional private nforork to vpn. security of mpls wn architectur is analyzed thorouguy in this thesis, including septheion of address space and route, hiding of the mpls core smicture, resistance of attacks, impossibi1ity of 1abe1 spoofing
安全性是虛擬專用網能否取代傳統專用網的關鍵,本文對mplsvpn體系結構的安全性進行了深入分析,包括地址空間和路由的隔離、隱藏mpls核心結構、抵抗攻擊、抵抗標記欺騙等,從而得出mplsvpn與atm framerelay具有相同安全級別的結論。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選,在pap基因的3 』端融入了綠色熒光蛋白gfp )基因,然後將融合基因克隆在植物表達載體pbi121上,再進行遺傳轉化,得轉基因棉花種子5000餘粒,將種子播種長到于葉展開時,先在黑暗中用紫外燈照射,查找表現綠色熒光的幼苗,然後再用地高辛( dig )標記的pap基因特異性探針對這些棉花進行點雜交,最後發現有8株棉花表現陽性反應,說明pap基因的確己經轉到了棉花的基因組中,其棉花黃萎病的抗性鑒定正在進行之中。The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test
經超聲波裂解,用尿素溶解包涵體,電泳純化后,利用elisa檢測vp _ 1外殼蛋白,表明具有一定抗原性;第四部分:應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交,來檢測那v的rna 。The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation. 2. following lps or seb was administered intraperitoneally, the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice. double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內給予細菌內毒素lps或腸毒素seb ,用免疫組織化學方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達,並採用雙標記技術觀察了1型il刁受體陽性神經元和加壓素及催產素表達的關系。In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗(洛夫林10 ) 、感( 5389 )不同的兩小麥( triticumaestivuml . )品種為材料,採用酶解法制備小麥葉肉原生質體,利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記,並探索了微管骨架標記的影響因素。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。. from the direct mutant of spirulina platensis ( sp - d ), we got high purity and activity phycobiliprotein which could grow crystals. the algae fluorescent probe prepared by coupling the above polyclonal antibody to phycobilipotein not only keeps the property of stronger anti - fluorescence quenching but also has the lower fluorescent background when it was used for labeling stoma cells of pea tendril
以原核表達的peac1為抗原制備了免疫活性較好的抗豌豆肌動蛋白的多克隆抗體,從螺旋藻中純化了高純度、高活性、能結晶的藻膽蛋白,將兩者偶聯制備的藻熒光探針,不僅保持了藻膽蛋白很強的抗熒光淬滅能力,而且用於豌豆卷須氣孔細胞熒光標記時有更低的熒光背景。( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice. the selectable marker gene is hyg gene
( 2 )表達載體pcar中目的基因由單子葉強啟動子水稻act1啟動子調控,選擇標記基因為潮黴素抗性基因。It was proved that the amount of immobilized antibody and the immunoactivity of bound antibodies could be well improved by colloidal au. hrp labeled antibody reacted with antigen, then hrp biocatalyzed dab ( 3, 3 ’ - diaminobenzidine ) in the presence of h _ 2o _ 2, resulting in an insoluble product onto the electrode surface, to achieve an obviously decreased frequency
在h _ 2o _ 2存在下,通過標記在抗人igg抗體上的辣根過氧化物酶( hrp )催化底物3 , 3 』 -聯苯二胺( dab ) ,反應生成不溶性產物沉積到石英晶振的au電極表面,達到質量放大的目的。In determining the optimal concentration of each antibody, we have used a strategy in which the capture antibody is first immobilized at a particular density on the predetermined site of membrane, and the labeled antibody is then varied to retain a maximum signal - to - noise ratio
在測定每個抗體的最佳濃度中,我們用到了這樣一個方案:先將捕獲抗體以特定的濃度固定在膜上預留的部位,然後改變標記抗體濃度得到最大的信號噪音比例。Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理,首先對抗a 、抗b單克隆抗體進行熒光標記,然後使熒光標記抗體與相應抗原(血痕)在最佳條件下結合,最後熒光顯微鏡鏡檢,判定血痕的血型。Objective to explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material
摘要目的探討採用熒光免疫標記抗體法測定血痕血型的可行性和優點。分享友人