檢核表 的英文怎麼說
中文拼音 [jiǎnhébiǎo]
檢核表
英文
cbcl-
Conclusions : prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen were successfully constracted, and the target proteins expressed by iptg induced in escherichia coli. as well as in eukaryocyte ( hepg2 and cos - 7 ), then their antigenity were detected
結論：截短的乙型肝炎表面抗原分子的原核和真核表達』重組質粒成功被構建及分別在人腸桿菌efl得到誘導表達和存貞核細胞ifj表達，並檢測劍其表達產物的抗原特性。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot
為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa
將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。An orientation checklist and three month progress review form accompanies the introduction form of non - exempt employees
一份新進員工簡報會議檢查表及為期三個月的試用期績效考核表，連同有加班費員工的簡介表格Recommendation for release or transfer may be submitted on form to be attached to the orientation checklist and three - month progress review
應送交相關表格，連同新進員工簡介訓練檢查表及三個月績效考核表，註明對該員工處置的建議或許是辭退或要求調職。If the extension is approved, probation advice form should be completed and attached to the orientation checklist and three - month progress review and processed as in any other instance of disciplinary probation
假如延長期限被核可，試用通知表格應填寫完成連同新進員工簡介訓練檢查表及三個月績效考核表，做為紀律案件的考量。Objective : to construct prokaryotic and eukaryocytic expression plasmids of the shortened hepatitis b surface antigen, and express the target proteins by iptg induced in escherichia coli
目的：構建截短的乙型肝炎表面抗原分子的原核和真核表達重組質粒，然後分別在大腸桿菌中誘導表達並純化表達蛋白及在真核細胞中表達目的基因，並檢測其抗原特性。Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection
用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核，並移植入假孕受體鼠，產生的65隻n代小鼠中，經pcr和southern雜交檢測，表明轉入的基因在12隻小鼠體內有整合段。The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。This reference mode can also offer a guideline to colleges, which want to set up the quality management system or / and which want to adapt the qms editor to iso9000 : 2000 editor. as the size, framework and process are various among the colleges, the author offers a reasonable assessment method in order to make the quality audit more efficient and accurate. to design a framework or syllabus of audit check sheets, which can be applied to all colleges, will reduce contrived error if the quality management systems of the colleges are audited by different auditors and will make the administration get an intuitionistic understand of the systems
由於各學校在規模、結構和所採用的過程不盡相同，為更好做好學校質量體系的審核工作，本文提出較為合理的評價方法，按iso9001 ： 2000標準質量管理體系的審核準則設計一個適用於各種學校的審核檢查表的框架或提綱對學校的iso9000質量管理體系進行評價，使不同審核員按此方法對學校的iso9000質量體系運行的真實狀況評價時，減少審核中的人為誤差，使主管機關對學校質量管理體系有較為直觀認識。These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5
方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ eGene silencing, a common phenomenon in phb production through nuclear transformation, had n ' t been observed in the chloroplast transgenic plants analyzed by northern dot blot
Northern點雜交檢測表明與phb合成相關的三個基因均能在轉錄水平表達，未出現核轉化中經常發生的「基因沉默」現象。Second, a prokaryotic expression construct, obtained from invitrogen transformed into prokaryotic and induced to express vp1 protein. the expressed vp1 fusion protein was purified by affinity chromatography using glutathione - agarose resin and used in elisa and western bolt analysis as the antigen. the elisa and western blot results showed that the anti - fmdv antibody was elicited specifically against vp1 antigen
第二，為了得到抗原蛋白，將vp1的原核表達質粒pgex - 4t - vp1轉化入大腸桿菌bl21中，經iptg誘導，裂解細胞後用瓊脂糖珠進行純化，用elisa和westernblot進行檢測，結果表明誘導表達出所需大小的融合蛋白。A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so
以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。After marking with gfp and hochest33342, we also observed the multinuclei and chromatin bridges in hela cells that were transfected with the c - terminal expressing plasmids corresponding to the human smc subunits hcap - e and hcap - c, respectively
進行rt一pcr檢測表明人集縮素smc亞基hcap一e和hcap一c的c一末端真核表達質粒在hela細胞內得到過表達。We even prepared a daily review checklist to help him remind himself at any moment
我們還為他訂定日常檢核表，讓他隨時提醒自己。Performance standards in building - checklist for briefing - contents of brief for building design
建築物性能標準.簡報檢核表.建築物設計簡報內容分享友人