毒質菌素 的英文怎麼說
中文拼音 [dúzhíjūnsù]
毒質菌素
英文
virosin-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考Impact of leakage electrolyte of eupatorium adenophorum caused by pathotoxins from lecanosticta acicola on growth of several fungi
松針褐斑病毒素誘發紫莖澤蘭電解質滲漏對幾種真菌的影響Phytoalexins are low molecular weight chemicals that plants produce and accumulate in response to infection especially of fungal origin. sakuranetin is a kind of flavanone phytoalexin isolated from ultraviolet - irradiated rice leaves. recent research work on flavanone phytoalexins represented by sakuranetin is reviewed. interesting novel structures, stucture - activity relationships and synthetic methods are discussed
植物抗毒素是植物受到外界病原微生物侵擾后所產生並積累的一類具有抗菌活性的小分子物質，櫻花素是從水稻稻瘟病感染組織中分離鑒定的一種黃烷酮類植物抗毒素.對以櫻花素為代表的水稻抗毒素及其類似物的結構與活性、黃烷酮類植物抗毒素合成方法的研究概況進行了綜述In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。A study on expression and purification of tdh - related hemolysin gene of vibrio parahaemolyticus
副溶血性弧菌直接溶血相關毒素蛋白的純化及性質初探Expression of thermostable direct hemolysin gene of vibrio parahaemolyticus in e. coli and activity of expressed product
副溶血性弧菌直接溶血毒素基因的原核表達及產物的性質Rich active mineral elements in bamboo salt can clean the skin completely, remove aged horniness, eliminate the toxin in the body, effectively resist and kill bacteria, and relieve itching
竹鹽中豐富的活性礦物質有助於徹底清潔皮膚，去除老化角質，清除體內毒素，並有效抵禦細菌入侵，除菌止癢。In spring, red wine in the bath will help dispel body toxin ; in summer, prunella spike and mint in the bath will reduce body heat, lower blood pressure, remove liver heat, improve eyesight, and clear heat and toxic substances ; in autumn, sulfur added in the spa helps dissolve cutin, soften the skin, remove swells, activate collaterals and alleviate pains ; in winter, coffee and ginger added in the spa may enrich the skin, and make it more resilient
如：春天紅酒浴有助排除體內毒素；夏天薄荷浴夏枯草浴防火降壓、清肝明目、疏散風熱、清熱解毒之功效；秋天硫磺浴，能溶解角質、軟化皮膚，並有消炎殺菌、通經活絡、祛寒止痛等功效；冬天，咖啡浴、老薑浴使皮膚更光澤、紅潤有彈性。Our goal is to express the ricin a chain ( rta ) and its modification rta - kdel ( er - retrival signal ) in escherichia coli
本論文旨在研究蓖麻毒素a鏈蛋白（ rta ）及其修飾體rta - kdel （內質網保留信號肽）在大腸桿菌中的表達。The major sanitizers ( chlorine, bromine, iodine, trisodium phosphate, quaternary ammonium compounds, acids, ozone ) used for vegetable decontamination were summarized. the advantages and disadvantages were also disoussed
摘要綜述了凈菜常用的消毒殺菌劑如鹵素及鹵素化合物、離子化合物質、有機酸、活性氧化合物等各自的優缺點。Bacillus thuringiensis israelensis produce crystal proteins which can be converted into toxins in the gut of the mosquito larva. the toxins act on larvae of limited species including mosquitoes, blackflies and non - biting midges
蘇雲金桿菌會產生蛋白質結晶體，而結晶體在蚊子幼蟲的腸內化為毒素，這些毒素會令某些品種包括蚊子墨蚋不叮不咬的蠓的幼蟲中毒。Bacillus thuringiensis israelensis produce crystal proteins which can be converted into toxins in the gut of the mosquito larva. the toxins act on larvae of limited species including mosquitoes, blackflies and non - biting midges. rodent control
蘇雲金桿菌會產生蛋白質結晶體，而結晶體在蚊子幼蟲的腸內化為毒素，這些毒素會令某些品種（包括蚊子、墨蚋、不叮不咬的蠓）的幼蟲中毒。Botulinum neurotoxin serotype a produced by the clostridium botulinum, are the most lethal substances known. a single molicule can abolish the function of a nerve cell. the toxins exert their action by blocking the release of the neurotransmitter, acetylcholie, at the neuromuscular junction, the blockage of bont / a makes neuromuscular weakness and flsccid paralysis, and lead to respiratory failure and death. botulinum is a serious public heath problem in developing countries around the world
A型肉毒毒素（ botulinumneurotoxinserotypea ， bont a ）是由肉毒梭菌產生的一種強烈的外毒素，是目前已知最毒的天然物質，一個單分子的肉毒素分子就能阻斷一個神經細胞的功能，通過阻斷運動神經末梢乙酰膽堿的釋放導致機體發生癱瘓，嚴重者發生呼吸衰竭而死亡。A conceptual approach including measurements of materials at rest ( step 1 ), measurements using a large rotating drum ( step 2 ) or a particle - flec ( step 2 ) and measurements at a workplace ( step 4 ) has been used to characterize the release of microbial components ( bacteria, fungi, actinomycetes, endotoxin or enzymes ) and particles from straw, wood chips or fungal cultures of different ages on gypsum boards
一套整體概念性的方法，包括物質在靜止時（步驟一） 、使用大轉動滾筒時（步驟二）或微粒逸散完時（步驟二） ，和工作場所（步驟四）進行量測，以描述由麥稈、木頭碎片或不同年份的石膏板上的真菌菌落所釋放的微生物組成（細菌、真菌、放線菌、內毒素或酵素）和微粒特性。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。Ingredients : botulinum toxin ( clostridium botulinum ), hyaluronic acid, hydrogen protein, grape polyphenols, aloe extract, seaweed extract, glycyrrhizic acid, licorice extract and vitamin e
成份：肉毒桿菌、玻尿酸、水解蛋白質、葡萄多酚萃取、蘆薈萃取、褐藻萃取、甘草酸、甘草萃取、維生素e 。Contents of the tests included examinations of heavy metals, pesticide residues, mycotoxins ( aflatoxin ), foreign matter, ash and water content, extractive and assay in the herbs concerned
檢測范圍包括藥材內重金屬、農藥殘留、黴菌毒素（黃麴黴素） 、雜質、灰分、水分及浸出物含量。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。New advances in the pharmacological and surgical treatment of neurologic diseases ( eg. botulinum toxin therapy, deep brain stimulation, motor cortex stimulation, etc ), and future development of cellular restorative therapy
包括神經疾病藥物及手術治療的最新進展（如肉毒桿菌素治療、深部腦刺激術、腦部運動皮質刺激術等） ，及神經再生治療的未來發展。分享友人