溶核蛋白 的英文怎麼說
中文拼音 [rónghédànbái]
溶核蛋白
英文
nucleolytin-
Chemonucleolysis is contraindicated in patients who are allergic to papaya or who have previously been injected with chymopapain
對番木瓜過敏的病人，或以前曾經注射過木瓜凝乳蛋白酶的人，化學核溶解法是禁忌證。The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體，在37條件下，洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體，包涵體在8mol l尿素中的溶解性非常好。Summary background data : soluble receptor proteins derived from the macrophage - monocyte lineage potentiate the inflammatory cytokine response early in ap
背景資料：從單核巨噬細胞系來源的可溶性受體蛋白可能是急性胰腺炎早期的炎癥細胞因子。With the increase of concentration of aqueous extract from peganum multisectum increased, root vigor, the contents of chlorophyll, soluble protein and nucleic acid in roots and shoots of alfalfa seedlings decreased, while the activities of protease and nuclease, the contents of o2 ( superscript - ), h2o2 and malondiadehyde ( mda ) increased, the activities of superoxide dismutase ( sod ), catalase ( cat ) and peroxidase ( pod ) first increased and then decreased
幼苗根系活力和葉綠素、可溶性蛋白質、核酸含量隨水浸液濃度的提高而降低，蛋白酶和核糖核酸酶活性及超氧陰離子（ o2 （上標- ） ） 、 h2o2和丙二醛（ mda ）含量則增加，超氧化物歧化酶（ sod ） 、過氧化氫酶（ cat ）和過氧化物酶（ pod ）活性呈先升后降變化。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Unicolor and lilium asiatic hybrids or cultivars in lilium asiatic hybrids were researched with their parents by karyotype, soluble protein, esterase isoenzyme and peroxidase isoenzyme. the results provided identification markers of cytology and biochemistry for hybridization at the early stage in lily breeding programs. the cluster analysis according to similarity coefficient of soluble protein and peroxidase showed that " yellow " and " omega " have the most closest relation in lilium asiatic hybrids
本研究對百合屬幾個植物的親緣關系進行了可溶性蛋白質、過氧化物酶同工酶分析，同時對亞洲百合雜交系內雜交及其與原種系間的雜交後代進行了核型、可溶性蛋白質、過氧化物酶和酯酶同工酶分析，以期為百合屬植物親緣關系分析提供生化依據，以及為雜交後代的早期鑒定提供細胞學、生化水平的檢測指標。These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5
方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ eThe coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna編碼區序列被克隆進原核表達載體pet - 30a ，並在大腸桿菌bl21 （ de3 ）中誘導表達，但過量表達的蛋白主要是以不溶性蛋白形式存在。By gene fusion and prokaryotic expression, we purified a pea actin isoform ( peac1 ), his - tagged peac1, his - tagged gfp and his - tagged peac1 - gfp from inclusion body. after filtrating a series of induction condition, we expressed and purified his - tagged peac1 with soluble form in a large amount
利用基因融合技術，原核表達並從包涵體中純化了豌豆肌動蛋白異型體peac1 、 his - taggedpeac1 、 his - taggedgfp以及his - taggedpeac1 - gfp ，並通過誘導條件的篩選達到了可溶性表達與大量純化his - taggedpeac1 - gfp的目的。It is a true solution of ions ( e. g. potassium, sodium, and chloride ), small molecules ( e. g. sugars, amino acids, and atp ), and a colloidal solution of large molecules ( e. g. proteins, lipids, and nucleic acids )
它是含離子（鉀離子，鈉離子還有氯離子） ，小分子（糖，氨基酸， atp ）還有大分子膠體（蛋白質，脂肪，核酸）的溶液。分享友人