溶菌性抗體 的英文怎麼說
中文拼音 [róngjūnxìngkàngtǐ]
溶菌性抗體
英文
bacteriolyic antibody-
The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form
本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。Two kinds of them are widely studied at present. one is the substance whose surface has micro - pore structure or crystal structure with excellent exchanging prosperity, such as silver - zeolite ; the other one generally select the glass with certainly chemical stability and soluble property, which are added a few ag +, zn2 +, cu2 + ion
目前研究最廣泛的抗菌材料有兩類，一類是物質表面具有微孔結構或具有離子交換性能良好的層狀晶體結構如銀沸石等；另一類通常是選用有一定化學穩定性和水溶性的磷酸鹽和硼酸鹽系統玻璃微載體的含銀、銅、鋅的抗菌玻璃。Oligochitosan is not only water - soluble, non - toxic, biocompatible but also possesses versatile functional properties of chitin and its derivatives, such as polyelectrolite properties, the presence of reactive functional groups, gel - forming ability, high adsorption capacity, bacteriostatic, fungistatic and antitumour influence, and thus it has attracted more and more attention in the field of biology. previous investigations have shown that chitin and its derivatives have immunoenhancement activities
殼寡糖是由3 10個n -乙酰氨基葡萄糖殘基或氨基葡萄糖殘基通過- 1 ， 4 -糖苷鍵連接而成的寡糖，它具有良好的水溶性、組織兼容性，在生物體內易降解，同時也具有抑菌、抗腫瘤、調血脂、調節免疫及活化腸道雙歧桿菌等多種生理功能，因此已成為眾多領域的研究熱點。2 ) basis of upon studies, we have also designed and sythesized the mutation ii of the cmiv that been greatly changed in the 3 " of the gene comparing with nature cmiv : the ht gf3 ( the third loop region of htgf2 specifically binding to egfr receptor ) was fused to 3 " of gene of cmiv through a flexible linker. the gene of the mutation ii of cmiv was sequenced and clonged to the vector of ptxfus to fuse to the 3 " of gene of thioredoxin
二、在以上研究的基礎上，對cmiv抗菌肽的c端進行較大的改造，即將與腫瘤細胞過度表達的表皮生長因子受體（ egfr ）具有高親和性的因子多肽tgf _ 3通過疏水柔性接頭連接在抗菌肽cmiv的c端，設計完整的基因，並在大腸桿菌中利用ptxfus表達載體與硫氧還蛋白進行可溶性融合蛋白表達。Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein
本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption. immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans, then eluted and further purified by columns of thiophilic gel. based on above experiments of antibody extraction, bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus
首先，利用變異鏈球菌（ s . mutans ）與其特異性抗體的吸附作用從水溶性蛋白組分中分離出卵黃抗體（ igy ） ，並將菌體上洗脫下來的igy用嗜硫色譜分離純化，得到高純度的細菌特異性抗體。And the fusion protein could been purified quickly by thiobond ? resin. this was key step to produce cmiv largely by fermentation. meanwhile, the structure of the mutation ii of cmiv showed a new way to design peptide of cecropin which have the targeting sequence to tumor cell
以上研究的意義： 1 、初步解決了抗菌肽cmiv突變體在大腸桿菌中高效可溶性表達的問題，為進一步大量生產cmiv基因突變體打下基礎； 2 、為進一步設計具有特異導向性的抗菌抗腫瘤cmiv突變體基因和表達以及以後進行動植物轉化打下基礎。As more particular functionality of fabric enhanced, more rationality and superiority are expressed in the selection of costume material. currently, we strive to choose one most suitable fabric for one relative style, as well as the most individual costume for the relative fabric, e. g. the function of moisture absorbing dry quickly is applied in sports wear, ultraviolet radiation resistance applied in leisure wear, anti - bacteria and expel adour applied in underwear. thus fabric is made in one with costume
面料特殊功能性的增加，大大提升了對服裝的選材更趨于理性與優越性。為每一款衣服選擇其最適合的面料，也為每一種面料選擇最彰顯其特性的服裝，從而使面料與服裝渾然溶為一體。比如：吸濕排汗在運動裝上的運用，抗紫外線在休閑裝上的運用，抗菌除臭在內衣上的運用等等。分享友人