無性系培養 的英文怎麼說

中文拼音 [wúxìngxìpéiyǎng]
無性系培養 英文
clonal culture
  • 無 : 無Ⅰ動詞（沒有） not have; there is not; be without Ⅱ名詞1 （沒有） nothing; nil 2 （姓氏） a surn...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 系 : 系動詞（打結; 扣） tie; fasten; do up; button up
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 無性 : [生物學] asexual; [植物學] neuter; agamo 無性繁殖 vegetative propagation; 無性生殖 asexual reprod...
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

    （ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
  3. I think all three topics are closely interrelated, with the latter two - getting your programmes right in content appeal, quality and scheduling ( programme characteristics ) and generating a massive pool of creative content - creators - the key mechanisms of providing effective counter - solutions to the problem of cultural and commercial colonisation by the global tv players

    我認為所有這三個話題與後面兩個是密切聯系的? ?使你們的節目在內容訴求、質量和節目安排（節目的特色）都適應無誤，培養能製作創造性節目內容人才，建立大規模人才資源庫? ?針對全球性電視玩家的文化和商業殖民化問題，提供有效的對策，有這樣一個提供對策的關鍵機制。
  4. The comparative advantages to develop inner mongolia beef cattle industry are the price, non - pollutions and vast grasslands etc. the disadvantages are as follows : be short of specific beef - cattle kind ; individual productivity ability ; meat nature promoting slowly ; raises method and management standard being fairly backward ; butcher, process, circulate, and the link aspect receives the technology restriction unfavorably factors such as serious etc. the suggestions for development of beef industry in inner mongolia are as follows : strengthening the bioengineering technology and setting up the inner mongolia beef cattle breeds system ; quickening the breed cultivating ; optimizing the breed structure ; effectively increasing the safe forage supplies ; strengthening epidemic disease prevention ; curing quarantining work ; developing the nutrition hygiene " green food " ; changing traditional raising method ; raising the management standard, and carrying on scope production

    發展內蒙古肉牛業具有價格、無污染、草原廣闊等優勢，但存在著專有肉用牛品種缺乏、個體生產性能和肉質提升緩慢、飼養方式和管理水平比較落後、屠宰、加工和流通環節方面受技術制約嚴重等不利因素，因此，要利用生物工程技術，建立內蒙古肉牛繁育體系，加快品種培育，優化品種結構；有效地增加安全飼料供應；強化疫病防治和檢疫工作，發展營養衛生「綠色食品」 ；轉變傳統飼養方式，提高飼養管理水平，進行規模化生產；重視開拓農村市場，促進內蒙古肉牛業發展。
  5. This paper describes the best growth regulator levels were selected for four suitable eucalyptus clones by trials of selecting suitable growth regulator levels on rooting in tissue culture for four eucalyptus clones

    本研究通過4個桉樹無性系組培生根培養最適生長調節劑的篩選試驗，篩選出各無性系生根培養的最佳生長調節劑濃度。
  6. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  7. It also has restrained the sustainable development of the mutton sheep industry in hebei, and affected further improving of the competitiveness of the mutton sheep industry in china. to solve these problems the paper points out creatively the concrete and formal management objectives, principles and methods. it ' s content is the matrix organizational structure, the open contractual operation manner, the personal and group inspiring plan, the management style of the personnel reward and training, the framework of production system, the process and function of mis, etc. through the preliminary study on formal management and mis, the paper tries to provide a theoretical base for stud ying on the transition from the traditional management to a modern one, in order to enforce the direction to the mutton sheep pen ' s modernization, industrialization, and intensity in management and operation

    個業的組織結構管理、人力資源管理、庫存管理、銷售管理、生產作業管理利生產經營方式管理等方面，無序、隨意、人治的管理現狀制約了肉羊養殖企業管理效率的提高、產品質量的升級，制約了河北省肉羊業的持續、穩定發展，也制約了中國肉羊業競爭力水平的再提高，為此，創新性地提出了關于肉羊養殖企業的矩陣組織結構、外包生產經營方式、員工個人和集體激勵計劃、員工薪酬和培訓的管理方式、生產系統框架、管理信息系統的流程及其功能等具體規范化管理的目標、原則和手段等。
  8. Three mistakes people could make in the construction on credibility : 1 ) some may mistakenly argue that morality could be improved in line with economic growth, but economic logic do not work for morality ; 2 ) some may advocate to re - implement traditional moral standards, including the altruism, to regulate corporate and / or personal actions in market economy ; 3 ) some may think that china ' s socialist market economy is nothing different from market economy adopted by western countries except for the name. suggestions on the construction of credibility in socialist market economy : 1 ) establish a new culture that is helpful for the development of socialist market economy. 2 ) update moral standards, transferring from altruism to egoism on condition that the behavior does not hurt others " interest

    建議從以下途徑出發建設社會主義市場經濟下的誠信體系: 1 、文化轉型，從傳統的、保守的農業文明轉向體現理性精神、契約精神、人本精神的現代工業文明，建設有中國特色的市場經濟文化; 2 、道德重建，應從傳統的無私利他、利己主義轉向已他兩利主義，使道德走向現實; 3 、制度的創新與完善，抓緊立法，嚴格執法，加強政府監管，樹立法律權威和政府權威，同時要建立和完善中介機構的管理制度、行業管理制度和企業自身的誠信管理制度; 4 、培育公民的誠信意識，政府應帶頭誠實守信; 5 、要注意培養企業家品格，塑造傳統與現代相結合的儒商精神。
  9. Objective to investigate the culturing technique for conjunctival epithelial cells and to evaluate the facibility of serum - free culture system on culturing conjunctival epithelial explants

    摘要目的研究結膜上皮細胞體外培養的方法，並進一步探索無血清培養系統用於結膜上皮細胞培養的可行性。
  10. The achievement of the students who had definite reading aim was better than that of those who had not ; 2 the students " comprehension monitoring ability in reading between grade 7 and grade 8 developed rapidly. however, when it came to grade 8 - 9, whether the trend of development would continue or not, it would depend on the reading conditions ; 3 be viewed from all aspects, there was always a close and inherent relation between junior middle school students " comprehension monitoring in reading and reading comprehension ; 4 be viewed from the plan before reading or the monitoring in reading or the evaluation after reading, the junior middle school students " comprehension monitoring ability in reading had reached a certain extent ; 5 bad reading habits, lack of necessary reading skills and relevant background knowledge, and indefinite reading aim, all of them could be the reasons that the student could n ' t remember what they were reading ( failing to monitor what they were reading ) ; 6 in order to improve the junior middle school students " comprehension monitoring ability in reading, some measures such as helping them form good reading habits, teaching them some necessary reading skills, and training them to good self - reading awareness, could be made ; 7 there were some ways could be used by teachers to improve the students " comprehension monitoring ability in reading

    有明確閱讀目的的要比沒有明確閱讀目的的要好； 2 、學生的閱讀理解監控能力從初一到初二有一個明顯的發展過程，而從初二到初三，則依閱讀條件的不同而有所差異； 3 、無論是從總體還是從局部進行分析，初中生的閱讀理解監控能力與閱讀理解之間有著緊密的內在關系； 4 、無論是從閱讀前的計劃性，還是閱讀中的監控性，以及閱讀（后）的評價性來看，初中生都已具備一定的閱讀理解監控能力； 5 、造成閱讀遺忘現象（理解監控失敗）的原因，主要有：沒有良好的閱讀習慣；缺乏必要的閱讀技巧；缺乏必要的背景知識及缺乏明確的閱讀目的； 6 、要提高學生的理解監控能力，可以從培養他們良好閱讀習慣，傳授必要閱讀策略和培養他們自主閱讀意識等方面著手； 7 、教師可以通過多種方式來幫助學生提高他們的理解監控能力。
  11. Methods : in cultured lung explants without serum, the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity, nitric oxide ( no ) content, superoxide dismutase ( sod ) level, malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods. results : 1. influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine, glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry, glu induced the changes of the ultrastruture of at ii iv cells

    方法：採用成年大鼠肺組織無血清培養，運用[ ~ 3h ] -膽堿摻入法測定ps主要脂質磷脂酰膽堿（ pc ）合成量； rt - pcr擴增檢測肺組織中pc合成限速酶磷酸膽堿二胞苷酰基轉移酶（ cct ） mrna含量；透射電子顯微鏡法觀察肺泡型上皮細胞和ps系統超微結構的變化；免疫組織化學染色檢測glu的受體nmdar1亞單位的表達；生化測定肺組織乳酸脫氫酶（ ldh ）釋放量和肺組織勻漿中一氧化氮合酶（ nos ）活性、一氧化氮（ no ）生成量、超氧化物歧化酶（ sod ）水平以及丙二醛（ mda ）含量。
  12. Optimization of synthesis condition of 25, 26, 27 - trimethoxy - 28 - hydrocalix 4 arene

    山荷葉的組織培養及無性系建立的研究
  13. These hairy roots grew well on ms agar medium or liquid medium withour growth regulator for over one year. they grew vigorously and produced numerous lateral branches. four hairy root clones, namely tra1, tra2, tra3 and tra4, were established

    毛狀根能在不含任何激素的ms固液體培養基中快速生長，且表現出多分支，多根毛，失去向地性生長等典型毛狀根特性；建立了tra1 ， tra2 ， tra3 ， tra4四個毛狀根無性系。
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