熒光增進劑 的英文怎麼說
中文拼音 [yíngguāngzēngjìnjì]
熒光增進劑
英文
fluorescence improver-
In this study, dna strand breaks in jm109 cells caused by - rays and the protective effect of lexa protein of deinococcus radiodurans have been studied by fluorometric assay of dna unwinding., the results indicated that the rate of dna strand breaks increased with the increase of - rays dose. however, when the concentration of lexa protein from 0. 1 g / ml to 10 g / ml, it still showed no protective effect on dna strand breaks, then lexa protein of deinococcus radiodurans was n ' t radioprotector
本研究採用了dna解旋熒光法檢測由射線所致的dna鏈斷裂的損傷程度以及抗輻射菌lexa蛋白的輻射防護作用，在輻照前加入不同濃度的lexa蛋白，檢測dna鏈的斷裂，結果表明dna鏈的斷裂程度隨著射線輻照劑量的增加而增強，抗輻射菌lexa蛋白對射線所致dna鏈斷裂的修復不起作用，進一步說明抗輻射菌lexa蛋白不起輻射防護作用。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。C - ptio could slightly enhance the fluorescent intensity of dcf stimulated by aba, while exogenous snp could not increase dcf fluorescence in guard cells
No的專一清除劑c - ptio對aba誘導的dcf熒光略有促進作用，但外源snp不能誘導胞內dcf熒光的增加。Suggesting that the larvae of spodoptera exigua died more quickly when fb - 28 were added to the virus. the larvae of spodoptera exigua inoculated as newly molted fourth instars ( 40s ) and at various hours after molting ( 316s ), we sacrificed larvae at various time intervals after inoculation to process the larvae for lacz expression in midgut by using tissue section techniques on early phase infection
此外，對于處于不同蟲齡的幼蟲進行病毒感染時發現：對3 ~ （ 16 ）幼蟲毒力生物測定時，熒光增白劑的增效作用達到50 ，但對4齡初的供試幼蟲進行測定，熒光增白劑的增效作用卻不很明顯，僅為10 。分享友人