片痘苗 的英文怎麼說
中文拼音 [piāndòumiáo]
片痘苗
英文
vacciv-
By using aa 385 - 365 fragment, an elisa system for the evaluation of post - e2 - vaccination humoral immune responses was also established, and was successfully applied to recombinant vaccinia virus - and dna - based vaccine research
利用aa385 - 565片段，建立了e2疫苗免疫后抗體反應的elisa評價體系，並已成功用於重組痘苗病毒疫苗和dna疫苗的研究。Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine
本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
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