牛核移植 的英文怎麼說
中文拼音 [niúhéyízhí]
牛核移植
英文
nuclear transplantation in cattle-
There were no significant difference in fusion rate, cleavage rate and blastocyte development rate with different enucleation time of oocytes during ivm16 - 24h ( p > 0. 05 )
在體外成熟16 24h期間,水牛卵母細胞去核時間的不同對核移植后的融合率,分裂率和囊胚發育率沒有顯著影響。4. rat - bovine interspecies somatic nuclear transfer 1 ) bovine cocs had been cultured in the maturation medium containing 8 - 10 % ocs in vitro for 22 - 24h, 60. 86 % - 61. 41 % of maturation oocytes can be obtained
(四)大鼠牛種間體細胞核移植研究的主要結果牛卵母細胞在含8 10發情牛血清( ocs )的成熟培養基培養22 24h ,能使602 ) we got 55. 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse. in addition, a same result can be obtained with low voltage + length impulse or strong voltage + short impulse
41的卵母細胞達到成熟:在1500 1800v cm , 30 40sin條件下,大鼠牛核移植重構胚能達到50廠0 55Effect of pre - activating bovine recipient oocytes before nuclear transfer on morphology change of donor nuclei
核移植前激活牛受體卵子對供體核形態變化的影響Effect of ethylenediaminetetraacetic acid on porcine oocyte maturation in vitro and subsequent early development after parthenogenetically activation
供體細胞處理方法對水牛核移植效果的影響This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide material to treat various diseases
本研究運用體細胞核移植的原理和技術,以成年大鼠的成纖維細胞為供體,去核小鼠和牛成熟卵母細胞為受體,構建種間核移植重組胚胎,研究種間體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。Compared with the cytoplasts matured in the maturation medium directly for 16 ~ 18h, the fusion rate, cleavage rate and blastocyst development of reconstructed embryos from oocytes cultured in 2mmol / l 6 - dmap for 3 - 5h prior to ivm did not differ significantly from that of reconstructed embryos from oocytes matured in the maturation medium directly for 16 ~ 18h
水牛卵母細胞先經水牛卵母細胞孤雌激活與體細胞核移植的研究6 dmap抑製成熟培養3 sh ,再在正常成熟培養液中成熟培養16 18h 。結果發現,與直接在正常成熟培養液中成熟培養16 18h的卵母細胞相比,其融合率,分裂率和囊胚發育率均無顯著影響。The results indicate that maturation rate of buffalo oocytes increase as the ivm duration prolongs, while the enucleation rate decreases, however, which has no significant difference on development of nt embryos with different enucleation time of oocytes during ivm16 ~ 24h
結果表明,水牛卵母細胞的成熟率隨著成熟時間的延長不斷提高,而去核率呈下降趨勢,但對其隨后的核移植效果沒有顯著影響(在ivm16 24h期間) 。分享友人