特異序列因子 的英文怎麼說

中文拼音 [tèyìxùlièyīnzi]
特異序列因子 英文
sequence specific factor
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  3. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  4. The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene. mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell

    第三章，根據一種常見的病毒煙草花葉病毒（ tmv ）的核酸序列設計了分子信標熒光探針，由於tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。
  5. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  6. We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment

    本研究從分子生物學角度入手，採用cytb作為分子標記，採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ，通過pcr技術，共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。
  7. The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps. then it is used in the following experiments. the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment

    本研究將含有人乳鐵蛋白（ hlf ）基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 』端調控序列的乳腺特異表達重組載體plncxhlf按常規方法提取、純化、 pcr 、酶切鑒定后，用於細胞表達和精子介導轉基因動物的研究。
  8. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  9. The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot

    4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。
  10. 2 heterogeneity and evolution of 5s rdna pattern of intragenomic and interspecific variation of 5s rdna hi five pines ( including subgenus strobus and pinus ) were studied through cloning and sequencing multiple 5s rdna copies the length of 5s rdna unit is 658 - 728 bp hi diploxylon pines while 499 - 521 bp hi the haploxylon p. bungeana

    Ssif7 ： na的序列變異與分子進化利用分子克隆和dna測序分析了油松、雲南松、馬尾松、白皮松和不同遺傳背景的高山松居群的ssilna基因序列變異及基因進化規律，得到以下主要結果： （ 1 ） ssrdna的結構特徵。
  11. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  12. 2. the dmrt1 gene of alligator sinensis was amplified by using a pair of special primers which can amplify the conservative motif ( dm domain ) of human dmrt1 gene, and then it was sequenced

    2 、參照人dmrt1基因dm盒保守區的序列，設計一對特異引物，擴增了揚子鱷的dmrt1基因，並對擴增產物進行了sscp分析和測序。
  13. Gene ( tscoxi ), structure protein gene ( tsdcn, tsmmip, tsb - actiri ), transcriptional factor gene ( tshmg1, tsdap5 ). the cdna library can be used to provide expressed sequence tags ( ests ). the stage - specific cdna library will be a useful resource for the study of gene structure, expression and regulatory during the early process of embroygensis of trionyx sinensis

    Blast分析表明， 12個序列代表5種類型的基因:核糖體蛋白基因（ tsrpl4 ， tsrp丈6 ，招天屍乙26 ） 、組織特異性表達的基因（鉆屍乃從招月叨火d ） 、線粒體基因（ tsc 「叨、結構蛋白基因伽dc從括人從石， ， ts刀一ctin ） 、編碼轉錄因子的基因（招月沏吟1 ， tsda屍5 ） 。
  14. The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs

    建立cdna表達文庫在一次永久保存基因資源的同時，可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因，進而克隆和表達這些基因，對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律，對揭示其抗藥性分子機理，搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制，對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。
  15. These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes

    對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2
  16. Cloning and sequencing of cucumis melo fruit - specific expression cucumisin gene promoter region

    甜瓜果實特異性表達黃瓜素基因啟動子區的克隆和序列分析
  17. The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared. by computer software, complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses, and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently

    通過計算機分析軟體，對我國ibv地方流行毒株lx4的主要結構基因全序列、分子特徵及遺傳變異進行分析比較，並初步分析預測傳染性支氣管炎病毒主要結構蛋白上可能存在的t細胞和b細胞表位。
  18. In order to avoid promoter - homology - mediated gene silencing, we isolated another seed - specific promoter nap300 from brassica napus h165 to replace 75 promoter in constructed expression cassettes

    將nap300與gus基因相連進行功能鑒定。熒光檢測和組織化學染色的結果都證明此僅300bp的dna序列足以調控基因進行種子特異性表達。
分享友人
分享到 Line

分享到臉書