特異轉錄因子 的英文怎麼說

中文拼音 [tèyìzhuǎnlùyīnzi]
特異轉錄因子 英文
special transcription factors
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 轉 : 轉構詞成分。
  • 錄 : Ⅰ名1 （用做記載物的名稱） record; register; collection; selections 2 （姓氏） a surname Ⅱ動詞1 （...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  • 轉錄 : [電學] transcribe; transfer; [聲學] mixing; rerecording; [生物學] transcription; duplicating轉錄...
  1. As a result of haart, hi - infected patients frequently deelop lipid abnormalities, including the accumulation of abdominal adiposity, features of the metabolic syndrome, and other factors that increase cardioascular risk

    作為強化的抗逆轉錄病毒的治療的結果， hi感染的患者經常出現脂類異常，包括腹壁多脂癥的蓄積，代謝綜合癥的特徵和其他的增加心血管危險的因子。
  2. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  3. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  4. As a member of an important transcription factor family, ap - 2 a expresses in specific tissue and binds with specific dna sequence

    Ap - 2作為一個重要的轉錄因子ap - 2家族的成員，具有組織表達特異性和dna結合特異性。
  5. Molecular cloning of human sperm surface protein p34h gene and semi - quantitative analysis of its expression in testis and epididymidis

    豬垂體特異性轉錄因子基因多態性的研究
  6. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  7. The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot

    4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。
  8. Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway

    摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。
  9. Recently, two distinct genes, dreb1 and dreb2, encoding dna binding proteins that specifically bind to the dre sequence of rd29a gene had been cloned from arabidopsis by using the one - hybrid screening technique

    它們編碼著與擬南芥rd29a基因的dre順式作用元件特異結合的轉錄因子，參與在乾旱，高鹽及低溫脅迫條件下的rd29a基因的表達調控作用。
  10. However there was little knowledge about the function of atdofl. 7, especially to stomatal movement. we constructed plant expression vector driven by camv35s promoter and obtained tl transgenic tobaccos

    為了深入理解調控氣孔運動的分子機制，除了對保衛細胞特異性表達啟動子進行研究外，還對與氣孔運動相關的轉錄因子進行了研究。
  11. 1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers

    將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。
  12. In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules

    研究發現呷基因的調控區存在多種轉錄因子結合位點，功能主要涉及組織特異性表達調控，細胞生長發育相關基因的表達調控，刺激反應基因的表達調控等； ndrgz蛋白在結構上屬于a小水解酶類折疊，折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似，提示ndrgz蛋白具有一定酶活性，可能參與細胞抗氧化應激反應，維持細， an ） armtbffiofbfochmilsyn ） mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡，參與內外源有毒物質的代謝等。
  13. Gene ( tscoxi ), structure protein gene ( tsdcn, tsmmip, tsb - actiri ), transcriptional factor gene ( tshmg1, tsdap5 ). the cdna library can be used to provide expressed sequence tags ( ests ). the stage - specific cdna library will be a useful resource for the study of gene structure, expression and regulatory during the early process of embroygensis of trionyx sinensis

    Blast分析表明， 12個序列代表5種類型的基因:核糖體蛋白基因（ tsrpl4 ， tsrp丈6 ，招天屍乙26 ） 、組織特異性表達的基因（鉆屍乃從招月叨火d ） 、線粒體基因（ tsc 「叨、結構蛋白基因伽dc從括人從石， ， ts刀一ctin ） 、編碼轉錄因子的基因（招月沏吟1 ， tsda屍5 ） 。
  14. The result of rt - pcr of transgenic plants showed : in transcriptional level, vp7 gene was expressed in leaves and fruits of 4 transgenic tomato plants under control of camv35s promoter ; vp7 gene was expressed in the tomato fruits not in the leaves of 3 transgenic tomato plants under control of fruit - specific tfp promoter

    Rt - pcr分析證明，在組成型啟動子camv35s控制下的5株轉基因植株的葉片和果實中，有4株vp7基因在轉錄水平上表達；而在果實特異表達啟動子tfp控制下的3株轉基因植株， vp7基因僅在果實中獲得轉錄水平上表達。
  15. A method of predicting human tumor peculiar promoter is introduced. by using transcriptional factor binding sites, this technique can receive a result well and truly

    摘要本文介紹了一種利用轉錄因子結合位元點應用計算機技術對人類腫瘤特異性啟動子進行預測的方法。實驗結果證明該方法具有較高的準確性。
  16. The semi - quantitative analysis in expression quantity of pit - 1 gene and related genes in xiang pigs

    香豬垂體特異性轉錄因子基因及其相關基因表達水平的半定量分析
  17. At first, total 31 transcription factors correlated to cell proliferation, differentiation, physiological stress and apoptosis, were selected ( see in article ) to use in the two - hybrid, and cloned into plasmid pact by using rt - pcr to amplify these transcription factors from fetal tissue cdna or stimulated lymphocyte cdna. as result, only six transcription factors atf3, atf4, e2f6, c - jun, c - fos and p53 were correctly cloned

    基於以上的背景信息，本文利用細胞雙雜交技術，對resrin在細胞周期中可能的作用的轉錄因子進行了篩選:一、首先從genebank中篩選了31個與細胞周期密切相關的轉錄因子，設計了特異性引物。從胎兒組織和刺激后的淋巴細胞中克隆了這些轉錄因子並構建於細胞雙雜交系統的pact質粒中。
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