病毒結構基因 的英文怎麼說

中文拼音 [bìngdújiēgòujīyīn]
病毒結構基因 英文
viral structural gene
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 構 : Ⅰ動詞1 （構造; 組合） construct; form; compose 2 （結成） fabricate; make up 3 （建造; 架屋） bui...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 病毒 : [醫學] virus; inframicrobe （濾過性）
  • 結構 : 1 （各組成部分的搭配形式） structure; composition; construction; formation; constitution; fabric;...
  1. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  2. Pp38 was found to have immunosuppressive effect on chicken in vivo and the copy number of 132 - bpr was found to be associated with the attenuation of the virus in vitro, meq was believed to be a potential oncogene, based on its leucine zipper structure and proline - rich domain characteristic of jun / fos family of transcription factors, and may plays an important role in the pathogenicity or oncogenicity of mdv

    其中， meq與132 - bpr兩個基因是mdv - 1所特有，已證實132 - bpr的拷貝數與毒株的體外致弱程度有關， pp38則被證實可抑制機體的免疫應答。 meq基因由於具有與致瘤基因jun fos家族類似的分子結構，因此，我們有理由認為meq基因在mdv致病、致瘤中可能發揮重要的作用。
  3. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  4. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  5. All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains, and there were many difference between velogenic strains and lentogenic strains. so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication, pathogenicity of ndv and the interaction between ndv and host cells, and the infectious cdna of velogenic strains ( eg. f48e9 ) was required to explain the relationships between structure and function

    本研究成功地獲得了ndvf48e9 t因組的核昔酸序列，並構建了表達ndvf48e9基因組cdna的低拷貝表達載體休f48e9 ，為構建新城疫病毒強毒株f48e9株的感染性cdna奠定了物質基礎，進一步研究ndv的生物學特性、結構與功能的關系；進一步探討影響ndv毒力的因素、以及研製新型疫苗載體提供了可靠保證。
  6. In terms with the principle of fusarium oxysporiun caused plant disease : bundles were blocked and fusarid acid killing cells was formed by hyphae so that caused water metabolism abnormal and plant wilting. in order to find out effective method of anti - fiisarium oxysporuin, long ya lillium was taken as material with plant tissue culture and genetic transformation techniques in this paper

    針對尖孢鐮刀菌的致病機理：菌絲阻塞維管束引起水分代謝失常和菌絲在植物體內產生毒素（鐮刀菌酸）損害膜結構造成代謝失常，從而導致植物萎焉。本實驗以龍牙百合為研究對象，應用細胞工程中的離體培養方法並結合轉基因技術，以期找到抗尖孢鐮刀菌的有效途徑。
  7. This study provides scientific theoretical information for the molecular epidemiology of pseudorabies in guangxi and lays a good foundation for constructing ge gene delected vaccine and the diagnostic method of identifying prv

    這些結果對我區偽狂犬病病毒的分子流行病學調查和構建ge基因缺失疫苗，建立偽狂犬病的鑒別診斷方法奠定了基礎。
  8. The transfer vector pbdtk - uni can be used for the construction of recombinant prv expressing foreign gene ( s ). postgraduate : tianzhijun specialty : preventive veterinary science supervisors : prof. li yijing prof. tong guangzhi

    以上結果表明所構建的具有自主知識產權的通用prv轉移載體pbdtk - uni是成功的，為利用該載體構建偽狂犬病病毒二價基因工程疫苗提供了技術平臺。
  9. The genome structure of tgev, pedv and prcv had high homology and it was difficult to diagnose tgev by traditional means. two studies were carried out to differentiate the tgev from prcv and investigate the epidemiology of tge

    Tgev與豬流行性腹瀉病毒（ pedv ） 、豬呼吸道冠狀病毒（ prcv ）在臨床癥狀上很相似，基因組結構上與tgev具有較高的同源性，傳統的方法很難鑒別。
  10. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  11. Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified

    為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。
  12. Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein

    經分析偽狂犬病病毒立即早期蛋白（ immediate - earlyprotein180 ， ie180 ）的蛋白質結構，發現ie180具有轉錄激活因子的結構特徵，能與類rna聚合酶結合， ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究，是考察ie180能否作為轉錄激活因子應用的關鍵。
  13. Based on the foundation research of the interaction of virus and host actin, we recombine gfp gene - a reporter gene - with 5c actin gene of drosophila melanogaster, a gfp - actin fusion gene was obtained

    本文在總結前人關于病毒與宿主肌動蛋白相互作用的研究的基礎上，利用綠色熒光蛋白基因為標記基因，與果蠅肌動蛋白基因5cactin基因融合，構成gfp - actin融合基因。
  14. Relatively, the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96. 8 % - 97. 2 % at dna or aa levels, than the homology among hk46 and 3 strains, strains gx8 / 99 more than 98. 4 % - 98. 6 % at dna or aa levels

    為研究病毒的核酸分子結構與其致病性的關系，本研究選取了在致死率上不同的4個ibdv野毒株，比較了它們的vpz基因高變區共494個堿基序列。
  15. The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1

    本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。
  16. Conclusion : 1 the 3 constructed adenovirus vectors shuttle plasmid of hcv structural gene

    結論如下： 1構建了3種hcv結構基因腺病毒載休穿梭質粒pad
  17. Study on establishment of cellular model transcripting hcv structural genes

    建立轉錄丙型肝炎病毒結構區基因細胞模型的研究
  18. Construction and packing of replication - defective adenovirus vectors expressing hcv structural proteins will play an important role in research of hcv and vaccination. hcv infection often result in persistent infection

    構建並包裝表達hcv結構基因的復制缺陷型腺病毒載體，在丙型肝炎的防治和hcv的研究中具有重要意義。
  19. The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared. by computer software, complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses, and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently

    通過計算機分析軟體，對我國ibv地方流行毒株lx4的主要結構基因全序列、分子特徵及遺傳變異進行分析比較，並初步分析預測傳染性支氣管炎病毒主要結構蛋白上可能存在的t細胞和b細胞表位。
  20. We constructed 3 kinds of shuttle plasmid encoding hcv structural proteins by means of molecular cloning technique ; based on it, 3 kinds of adenovirus vectors of hcv structural proteins were packed, screened and identified. we also selected and synthesized 5 kinds of ctl epitope peptides of hcv structural gene which induced 5 kinds of corresponding hcv specific ctl, which were evaluated by experiments in vivo and in vitro

    本研究利用分子克隆技術，構建了三種hcv結構蛋白腺病毒載體穿梭質粒，並以此為基礎包裝、篩選和鑒定了三種hcv結構蛋白腺病毒表達載體，選擇併合成了hcv結構基因區5條ctl表位多肽，並以此誘導了5種hcv特異性ctl ，並對其進行了體內外的實驗測定。
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