白色載體 的英文怎麼說
中文拼音 [báishǎizǎitǐ]
白色載體
英文
white support-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus
在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays
為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Construction of recombinant adeno - associated virus vector expressing glial cell line - derived neurotrophic factor labeled by green fluorescent protein
綠色熒光蛋白標記的大鼠膠質細胞源性神經營養因子重組腺病毒載體的構建及其表達In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing
實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。After the induction of iptg, the extracellular proteins of pet22asg / bl21 ( de3 ) plyss showed blue when treated with gus staining solution ; but the extracellular proteins of ck : bl21 ( de3 ) plyss showed no color change with the same treat. these results verified the function of secreting protein by grb - ast signal peptide
將表達載體pet22asg導入表達宿主菌e . colibl21 （ de3 ） plyss ，經iptg誘導表達，其胞外蛋白以gus染色液處理顯藍色；而對照空宿主菌bl21 （ de3 ） plyss誘導表達后，經同樣的處理，顏色無明顯變化，這初步證實了ast信號肽具有一定的分泌蛋白的功能。Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province. methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province. different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers
方法：從桂北五步蛇毒腺中抽提總rna ，利用不同的引物，採用一步法（ rt - pcr和pcr在同一管內進行）擴增出不同的dna條帶，利用平端連接的方法將pcr擴增產物克隆至pgem - teasy載體，轉化大腸桿菌jm109 ，挑選白色菌落提取質粒，用pcr對其進行鑒定，直接利用純化pcr產物或提取陽性菌落質粒進行測序。In this paper, it gives the mechanism of charge - coupled devices and its plane borehole pattern, and show how the digital camera work. 2. it studies the reconstruction algorithm for digital camera. the theory of color is firstly introduced, then algorithm are studied in this paper, these algorithm are subtracting of fix - pattern noise, color reconstruction auto gain control algorithm and auto white balance algorithm, then designed a download soft for digital camera
首先對顏色理論作了簡單的介紹，然後根據數碼相機的工作過程對各階段所用的圖像處理演算法進行了研究，這些演算法包括固定圖案噪聲的去除演算法、自增益控制演算法、白平衡調整演算法、顏色重建演算法以及灰度拉伸演算法，最後利用這些演算法開發了一個數碼相機的下載軟體。Led stands for light emitting diode, a kind of semiconductor which is used to give and receive the electronic signal into infrared rays or light, using the characteristics of compound semiconductor. this is used for household appliances, remote controller, electric bulletin board, various kinds of automation appliances
它是利用固體半導體晶元作為發光材料，在半導體中通過載流子發生復合放出過剩的能量而引起光子發射，直接發出紅黃藍綠青橙紫白色的光。In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。A cdna subtractive library with high subtractive efficiency of repeated + gz exposures in rat brain was constructed with suppression subtractive hybridization ( ssh ). the cdna subtractive library after amplification included 100 blue clones and 400 white clones, 75 ones of which were selected to prepare for plasmid. identification of the clones with restriction endonuclease cleavage showed most of them had been cloned to the vector
構建高消減效率的+ gz重復暴露大鼠腦cdna消減文庫，擴增后cdna文庫包含約400個白色克隆和100個藍色克隆，克隆飽滿清晰，隨機挑取75個白色克隆，制備質粒后，以ecor酶切分析，表明大部分克隆入質粒載體。Gfp can be expressed in the host enterococcus facecalis and lactobacillus plantarum we isolated using the expression vectors we constructed the expression level depends on the nisin concentration to some extent
Ow洲poi114no例烴們表達載體在我們分離到的受體菌efqecalis和l plantarum中能夠表達綠色熒光蛋白oppx表達的水平依賴於一定范圍濃度的nistn誘導。This paper investigate modem buddhist literature through the aspects of the definition, carrier, group of the writer, type, theme, topic, expression, aesthetics, historical origin and the relationship with other temporal literature, trying to fill up the empty of buddhist literature in the academic world
摘要論文從當代佛教文學的界定、載體、作者群體、體裁、主題、題材、表現手法、審美特色、歷史淵源、與其他文學比較體的關系等方面對當代佛教文學加以研究，試圖填補此方面學術研究的空白。The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively
將所構建的單鏈抗體基因克隆入綠色熒光蛋白融合表達載體pegfp - n3 ，瞬時轉染cos - 7細胞，分析其表達情況。Construction and primary application of enhanced green fluorescent protein adenovirus expression vector in the tracking and quantitative analysis of transplanted stem cells
增強型綠色熒光蛋白腺病毒表達載體構建及其在移植幹細胞示蹤和定量中的初步應用分享友人