白質后連合 的英文怎麼說

中文拼音 [báizhíhòuliángě]
白質后連合 英文
commissura alba
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 連 : Ⅰ動詞1 （連接） link; join; connect 2 （連累） involve （in trouble）; implicate 3 [方言] （縫） ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 連合 : coalesce; inosculation連合處 commissure; 連合分佈 joint distribution; 連合活字 logotype; 連合線 linea commissural
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %

    本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。
  3. Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid

    Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。
  4. After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3, the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains, 4 positive clones were screened by restriction enzyme analysis, dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid, which was the same as that of pbd - i and pbd - ii gene respectively, and its reading frame was correct, thus its could be used to express fusion protein

    將pbd - 、 pbd -基因與表達載體pinpoint ~ （ tm ） xa - 3連結后獲得的重組質粒ppd - 1 、 ppd - 2轉化于大腸桿菌jm109中。經抽提質粒、酶切分析及pcr擴增，分別篩選到4個陽性克隆，將其中二個陽性克隆由測序分析，證實1個含pbd i基因片斷， 1個含pbd 11基因片斷，且閱讀框正確，可用於融合蛋白表達。
  5. I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method, ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid, the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e. coli. the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses

    4 . h隊b一p及其突變體高效表達工程菌的構建:將用pcr擴增的h隊b一p 、 h獄b一p35 、 h隊b一p34基因，分別與pinpointxa一3質粒連接，轉化jm109大腸埃希菌，獲得的工程菌在表達融合蛋白時不穩定，第5代后即見不到表達條帶。
  6. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  7. Objective to prepare ea and ed protein of p - galactosidase with gst fusion protein by pgex - 4t - 2 expression system, and study its a complementation activity. to lay a good foundation for further study of cedia and utilization in expression immunoassay. methods an artificial synthesized dna segment coding for residues 6 - 56 ( modified ) of p - galactosidase ( ed protein ) was ligated to pgex - 4t - 2 vector

    實驗方法通過人工合成編碼d半乳糖昔酶ed蛋白的dna並插入原核表達載體pgex 4t 2 ；從陀v p galactosldasecontrolvector質粒中用sal及earl進行雙酶切得到編碼卜半乳糖昔酶a一受體（ ea ）的大部分dna ， n端再加上約60hp人工合成洲a ，連接后轉入pgex葉t億載體中。
  8. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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