目的基因 的英文怎麼說

中文拼音 [mùdejīyīn]
目的基因 英文
target dna
  • 目 : Ⅰ名詞1 （眼睛） eye 2 （大項中再分的小項） item 3 [生物學] （把同一綱的生物按彼此相似的特徵分為幾...
  • 的 : 4次方是 The fourth power of 2 is direction
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 目的 : purpose; aim; goal; objective; end
  1. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  2. Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle, low cost and high benefit. though traditional breeding technology has been used for a long time. now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known.

    關于花色結構基因查爾酮異構酶chi （ chalconeisomerase ）基因對花色改變的直接報道很少，因此，本論文選用了chi基因為目的基因，以純深紅色和純白色矮牽牛（ petuniahybidavilm . ）為材料，研究了chi基因的共抑制和反義抑制以及表達產物增加對花色改良的作用，並在花色改變植株中首次觀察到花器官變異。
  3. According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified

    以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
  4. These results suggested that dgp1 could drive target gene expressed mainly in guard cells when plant is subjected to drought stress

    這些結果證明，在植物遭遇乾旱脅迫時， dgp1啟動子可以驅動目的基因在保衛細胞中特異性表達。
  5. Target histidine genes

    組氨酸目的基因
  6. However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

    利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。
  7. The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling

    利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
  8. The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli

    結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。
  9. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  10. The supernate of virus culture was used as templet in overlapping pcr, then the interesting gene was obtained

    利用重組pcr的方法，以病毒培養液上清為模板，把二個目的基因串聯在一起。
  11. On the basis of the mentioned above and the real - time pcr principal, we have designed and optimized the primers and probes, set up the real - time qualitative pcr detection system. meanwhile, using the endogenous zein - maize and lectin - soybean as interior collate gene, epsps and cryia ( b ) as target genes, set up the real - time quantitative pcr detection system

    在此基礎上，根據熒光pcr原理，設計、優化引物和熒光探針，建立了熒光pcr定性檢測方法體系，並以玉米內源基因（ zein ） 、大豆內源基因（ lectin ）作為內標基因，以epsps和cryia （ b ）作為目的基因，建立了熒光pcr定量檢測方法體系。
  12. Actin filaments can not be visualized in other cell types, including stem epidermal cell, root fair cell and pollen

    在轉基因植株的其它部位，例如莖表皮細胞、根毛細胞和花粉粒中，未檢測到目的基因的表達。
  13. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  14. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。
  15. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
  16. ( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice. the selectable marker gene is hyg gene

    （ 2 ）表達載體pcar中目的基因由單子葉強啟動子水稻act1啟動子調控，選擇標記基因為潮黴素抗性基因。
  17. 4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis

    通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，並且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。
  18. The ms188 gene was finely mapped. a total of 8 new indel markers were designed to map msl88 using a segregating population with a total of 2135 male sterile progenies. ms188 was finally mapped to a region of 95. 8kb between the molecular marker mda7 and k24c1

    在與ms188連鎖的分子標記mc015附近設計了8個indel分子標記，對遺傳群體中2135株不育植株進行基因型分析，最後將目的基因定位於第五條染色體分子標記mda7和k24c1之間95 . 8kb的區間內。
  19. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  20. According to reference, this research is the first time to transfer practically productive aim - gene to tetraploid black locust, and it is also the first time to transfer badh gene to forest - tree. this research provide a useful way in improving resistance to soline - alkali and drought of forest tree

    據資料檢索，該研究屬首次將具有生產意義的目的基因導入四倍體刺槐，也是首次將甜菜堿醛脫氫酶基因導入林木樹種，在培育耐鹽抗旱型林木品種方面進行了有益的探索。
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