Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel
結果如下:皮下包埋卜周者,無細胞
真皮基質漸與周圍組織粘附,顏色由蒼白轉紅;皮下包埋3周者,無細胞
真皮基質與周圍組織緊密枯附,盾晰
葉辯;術后卜周,包埋的基質面積變化較包埋前無統計學差異o川0引,術后4周包埋的無細胞
真皮基質面積較包埋前縮小j刃刀5 ) 。光鏡下術后卜周,宿主的淋巳組織細胞、成纖維細胞浸入生長,釉附在膠原纖維上,少量血管內皮細胞浸入基質;術后34周,無細胞
真皮基質內較多的血管形成,故可認為無細胞
真皮基質免疫原性低,能誘導宿主的成纖維細胞、巨噬細胞浸入生長,為一種新型的
真皮替代物。第四部分無細胞
真皮基質與自體斷層皮片復合移棺的研究, sd大鼠10隻,在其背部卜方造成全厚皮膚缺損的創面
The germination of two seeds of hybrids was different form their parents. seeds of their parents were epigeal germination, but two seeds germination of hybrids which were derived form l davidii var. unicolor x ' yellow " and l davidii var
雜交種子萌發出現了不同於雙親的現象,親本種子萌發都屬于子
葉出土型,而蘭州百合黃色及蘭州百合克得利亞中各有一粒種子的萌發,其子
葉不生長,直接長出
真葉。
The highest vt and p valt in true leaf were accompanied two hybridizable polypeptides of aox protein, 35kd and 38kd respectively. the next was cotyledon vt and p valt with only one 38kd hybridizable polypeptide of aox protein. hypocotyl vt and p valt were the lowest and its immunobloting band was similar to that of cotyledon, but the expressive amount of 38kd protein was less than that of cotyledon
綠豆幼苗不同器官的有關呼吸參數測定結果與aox表達的western分析基本一致:
真葉的v _ t特別是v _ ( alt )最高,它也具有35kd和38kd的aox的雜交多肽;其次是子
葉的v _ t和v _ ( alt ) ,且在子
葉中,只見一條分子量為38kd的aox多肽;下胚軸的v _ t和v _ ( alt )都最低, western雜交顯示也只有一條分子量為38kd的多肽,而且表達量也較少。
A. implicatum could distribute in leaf sheaths, leaf blades, stems, roots and seeds, and could be transmitted by plant seeds, proved by the pcr detection method
利用該pcr檢測法探明了旗草內生
真菌a implicatum是通過種子傳遞的,其在植物體內可分佈於根、莖、
葉鞘、
葉片、以及種子。
Verrucous carcinoma is composed of lobules of mature squamous epithelium with minimal atypia, but they are ulcerating or fungating
疣狀癌由小
葉狀的具有輕度異型性的成熟鱗狀上皮組成,並且呈潰瘍或
真菌樣生長。
The species were separately caragana korshinskii hydysarum scoparim artemisia ordosica in shrubs layer, eragrostis poaeoides bassia dasyphylla in herbs layer, bryum argenteum barbula ditrichodies in microbiotic crusts layer. and the dominant species of them were artemisia ordosica eragrostis poaeoides bryum argenteum respectively. due to the different time and the topographical varieties in the fixing - sand region, the horizontal structure of artificial vegetation manifested out the mosaic characteristics
灌木片層的主要植物種是檸條( caraganakorshinskii ) 、花棒( hydysarumscoparim ) 、油蒿( artemisiaordosica )等灌木植物,其中優勢種為油蒿;草本片層的主要植物種有小畫眉草( eragrostispoaeoides ) 、霧冰藜( bassiadasyphylla )等,其中優勢種是小畫眉草;生物結皮由藻類和苔鮮類構成,其中,銀
葉真鮮( bryumargenteum夕為固沙植被區鮮類地被層優勢成分。
The number of minarets on a mosque indicates whether it is shiite and sunni
清
真寺宣禮塔的數量代表,它是什
葉派或遜尼派。
7 - 10 days " seedlings after the seeds germinated, the meristem in the stem apex functioned to form true leaf primordium, meanwhile, the vessel elements which differentiated from parenchymatous cells of the middle and upper portion of the cnz connected with the vessel elements from procambium of epcotyl - shoot region
種子萌發7 - 10天,幼苗的莖端分生組織活動形成
真葉雛形,此時由子
葉節區中、上部薄壁細胞的轉分化形成的導管分子與上胚軸-苗區原形層形成的導管分子發生連接。
Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba, cppu, tdz or kt ) alone or in combination with naa or iba, and the adventitious buds regeneration rate was evaluated.
以榨菜子
葉、
真葉為外植體進行離體培養,用ba 、 cppu 、 tdz和kt等細胞分裂素和生長素naa 、 iba組合誘導子
葉、
真葉再生不定芽試驗。
6. the plant is comparatively sensitive in two leaves period. so we can use the dose reaction to so2 in two leaves period as the injury threshold value to classify resistant ability type
6 .植株在2片
真葉期對50 :比較敏感,因此可以採用植物在2片
真葉期對50 :的劑量反應作為受害閡值來劃分抗性類型。
The periods of time, in which temperautre elements have greatest influence on cotton climatic output in akesu cotton region, are from cotton flowering to full flower stage in summer ( july 14 to july 28 ) and from later spring to middle summer ( may 17 to july 24 ), the later is the period of time from the first leaf to full flower stage
溫度要素對阿克蘇地區的棉花氣候產量有最大影響的時段是在盛夏的棉花開花?開花盛期( 7月14日7月28日) ,其次為春末和夏季的前中期( 5月17日7月24日)的屬于第一
真葉?開花盛期的時段。
According to the injury threshold value in two real leaves period, divided the stuff plats into four types : 1 ) the injury threshold value of the plants sensitive to so2 15ppm 3h ; 2 ) the comparatively sensitive injury threshold value 15ppm 3h - 20ppm 3h ; 3 ) the comparatively strong resistant injury threshold value 20ppm 3h - 25ppm 3h ; 4 ) the strong resistant injury threshold value 25ppm 3h
根據2片
真葉期傷害閻值,將供試的植物抗性分為4類: l )對50 :敏感的植物受害閡值( 15ppmx3h ; 2 )較敏感的受害j司值15ppmx3卜zoppmx3h ; 3 )杭性較強的受害j司值zoppn , x3h一25ppmx3h : 4 )抗性強的受害閡值> 25ppmx3h 。
2. the sensitivity to so2 differs in various plant growth period. the resistant ability to so2 in cotyledon period > real leaves period. the ability in two real leaves and four real leaves have n ' t clearly difference
植物不同生育時期對so _ 2的敏感性不同,植物對so _ 2的抗性是子葉期真葉期, 2片真葉期和4片真葉期對so _ 2的抗性差異不顯著。