硫解酶 的英文怎麼說
中文拼音 [liújiě]
硫解酶
英文
thiolase-
Determination of the catalytic structures of methyl parathion hydrolase
甲基對硫磷水解酶參與催化相關結構的研究Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase
3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱,先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析,最終使酶提純10倍,最終得率為17 . 4 。Then, nap300 and 75 were fused in a similar way to phba gene encoding 3 - ketothiolase for a direct comparison. the result indicated that the two promoter ' s expression efficiency reached a peak at the same developmental stage of tobacco, which means they have the advantage of being used simultaneously for expressing different foreign genes in plant
將nap300 、 7s分別與phba基因(編碼3 -酮硫裂解酶)相連,在相似表達環境中對二者功能進行比較,發現兩個啟動子表達模式基本相同並在同一時期達到活性高峰,因此nap300可用於改善phb合成基因在植物體內的表達調控。To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
雙胸蚓組織中dna酶的分離純化採用蔗糖溶解雙胸蚓,並選擇性酸變性制備雙胸蚓組織粗提取液,再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素( de52 )柱層析及超濾膜分級分離對雙胸蚓組織中dna酶進行分離純化。4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase
表達載體pbv - a 、 pbv - b和pbv - c經誘導表達,發現所表達的蛋白均為可溶性蛋白,沒有包涵體出現;蛋白經sds ? page分析表明,基因phaa表達的蛋白分子量為42kda ,與?酮硫裂解酶分子量大小一致;基因phab表達的蛋白分子量為26kda ,與乙酰乙酰coa還原酶分子量大小一致;基因phac表達的蛋白分子量為63kda ,與pha合成酶分子量大小一致。The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively
我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析,並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物,從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子,其在sds - page電泳中的分子量約為98 . 5kda 。The crude cellulases from liquid fermentation of b - 6 and ass. 3711 were isolated and purified by ( nh4 ) so4 precipitation, sephadex g - 100 and deae - sepharose cl 6b column chromatography. the cmcase components were purified and some of their physical and chemical properties were studied
本文將液體發酵的酶液經硫酸銨分級沉澱、柱層析后得電泳純cmcase組分,並對as3 . 3711和b - 6來源的cmcase酶解動力學和理化性質作了比較研究。Effects of fenvalerate, phoxim and their mixture on metabolic enzymes in helicoverpa armigera h 252 ; bner
辛硫磷及其混劑對棉鈴蟲解毒代謝酶的影響分享友人