等電點 的英文怎麼說
中文拼音 [děngdiàndiǎn]
等電點
英文
equipotential point-
The purple brown granules of hybridized signal were found in different stages of spermatogenesis cell
4kb ,編碼由295個氨基酸組成、分子量為33579 、等電點為9This ph is termed isoelectric point, at which proteins show their minimum solubility.
這個PH稱為等電點,蛋白質在這一點上溶解度最小。This ph is termed isoelectric point, at which proteins show their minimum solubility
這個ph稱為等電點,蛋白質在這一點上溶解度最小。Objective : to determine the amino acid composition and isoelectric point of porcine platelet - derived growth factor
摘要目的:測定豬血小板衍生生長因子的氨基酸組成和等電點。By race pcr, amplified products were obtained with all the gene - specific primers and the adaptor primers
由氨基酸序列推斷的ejo基因編碼蛋白的等電點為6 18 ,分子量為28A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase
連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ,由二個相同的亞基組成,亞基分子量為」 . ikda ,等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白,其中糖含量為17 . 44 % ,蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素It also had common features of plant nsltps ( non - specific lipid transfer proteins ) such as eight conserved cysteine positions, high isoelectric points ( 8. 9 ) and lack of tryptophans
其推測的氨基酸序列與genbank中注冊的ltp蛋白前體的同源性達63 ,且具有植物lh蛋白共同特徵,如8個保守的半肌氨酸位點,較高的等電點oi 8The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明:麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白,分子量分別為181kd和136kd ; sds - page電泳分析:存在3條雌特異性帶,其分子量為123kd 、 120kd和91kd ,由此,可推定卵黃原蛋白( vitellogenin , vg )和卵黃磷蛋白( vitellin , vn )由2個蛋白組成,其中分子量較大的蛋白由2個亞基組成;雙向電泳結果顯示,在120kd附近有兩個特異性點,其等電點為5 . 5和5 . 7 ;雙擴散表明,麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應,而與雄蜂血淋巴無免疫反應,說明了vg與vn具有免疫同源性,是雌特異性蛋白,且由脂肪體合成。Mineralization of soil organic carbon and its motivating factors to the dragon spruce forest and alpine meadows of the qilian mountains
電泳圖譜共分離出34條不同遷移率的譜帶;依據蛋白電泳圖譜等電點Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。Using a pair of special primers, whole sequence of m gene of b95 were amplified by rt - pcr and be sequenced after being directly inserted in plasmid vector pbluescript sk
結果表明: b95m蛋白基因全長1232bp ,共編碼364個氨基酸,其中堿性氨基酸有47個,酸性氨基酸有30個,等電點為9At last, we got the pure protein with the determination of electrophoresis. the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis. the isoelectric point is 8. 6 ?. 3
經sds凝膠電泳和凝膠過濾層析兩種方法測定該酶的分子量分別為33113dalton和28840dalton ;其等電點pi = 8 . 6 0 . 3 。Isoelectric focusing a technique used in electrophoresis to separate amphoteric molecules ( able to combine with either acids or bases )
等電點聚焦:是一種利用電泳技術分離兩性分子(能與酸或堿結合)的技術。Qd3. among them, six halocins ( hal c6, hal c8, hal c9, hal c16, hal cl7 and hal c22 ) displayed wide inhibitory spectra and could inhibit the growth of most ( > 10 ) haloarchaeal strains tested
純化后的嗜鹽菌素c8在tricinesds - page上表現為單一條帶,分子量大小為6 . 3kda ,等電聚焦測得其等電點為4 . 4左右。Methods : in the present experiment, acid dehydroxy amino acid analysis and isoelectric focusing in polyacylamide gels were used and the amino acid composition and isoelectric point of porcine platelet - derived growth factor were studied
方法:用酸水解及聚丙烯酰胺凝膠等電聚焦的方法測定豬血小板衍生生長因子的氨基酸含量、組成成分和等電點。Dot blot and northern blot studies revealed that amphihmgb expression is restricted mainly to ovaries and guts in adult amphioxus while it is highly expression during embryogenesis
文昌魚amphihmgb蛋白不含c ?端酸性尾巴,為一堿性蛋白,等電點為9 . 88 。In this study we firstly determined the time when the chitinase of helicoverpa armlerra produced in high activity and we cloned a cdna encoding a chitinase of helicoverpa armlerra by race method which was about l. 6kb and included complete 5 ' - end cap and 5 ' - utr
利用計算機分析該核昔酸序列,可知其編碼框為1341個核昔酸,編碼50kd含446個氨基酸的蛋白,推導等電點為phi9 575 ,為堿性蛋白。The results of sequencing and structural analysis showed that the gene, named crylaa14 by international nomenclature committee of bt 5 - endotoxin, was 3552 bp and the open reading frame encoded a 133. 7 kda protein with 1183 amino acids
序列測定結果表明該基因編碼區為3552bps ,編碼1183個氨基酸,分子量為133 . 7kda ,等電點為pi4 . 755 。該基因序列已在genbank注冊, accessionnumber為ay197341 ,並被國際bt殺蟲晶體蛋白基因命名委員會正式命名為cry1aa14 。Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp. it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level. this gene is a membrane protein which has one signal peptide, seven transmembrane helices, three n - glycosylation sites and one o - glycosylation site
序列分析表明,該基因的cdna全長為1398bp ,開放閱讀框為1095bp ,編碼一個364個氨基酸的多肽,與番茄和豌豆中的該家族基因分別具有71和69的氨基酸同源性,是一種膜蛋白,具有1個信號肽序列, 7個跨膜螺旋, 3個n ?糖基化位點和1個o ?糖基化位點,分子量大約為39 . 174kd ,等電點為8 . 07 。This feature is used to separate amino acids for analysis by chromatography and other methods
每個氨基酸都有自己的等電點,等電點的條件是凈電荷為零。分享友人