篩析表 的英文怎麼說

中文拼音 [shāixībiǎo]
篩析表 英文
size plot
  • 篩 : 名詞[書面語] （植物名） sedge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated

    Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。
  3. Using exploratory and confirmatory factor analysis, the factor structure of the extraversion dimensions has been identified through investigating 1420 subjects ; the items were selected and ceq was developed

    通過對1420名被試的測查，結合採用探索性因素分析和驗證性因素分析確定外向性維度的因素結構界定基本內涵，篩選項目編制外向性量表。
  4. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. From lots of models, this paper chooses seven models - model of mander, model of zhangxiuqin, model of sheikh, model of park, model of saatcioglu, model of fafitis and model of yuanjingen, which express the mechanics capability of confinement concrete perfectly and representatively. the paper modified some incorrect points of the models after studying them and some different hysteretic rules - hysteretic rules of park, hysteretic rules of blakeley, hysteretic rules of mander etc. were added to the models. on the base of above, the models were programmed and added in the program based on the column - beam element of the fiber model

    本文從大量的約束混凝土本構模型中篩選出具有代表性的七種模型，即mander模型、張秀琴模型、 sheikh模型、 park模型、 saatcioglu模型、 fafitis模型和袁錦根模型作為考察和研究對象，對部分模型局部明顯不當的地方進行了修改，然後在各模型中添加了不同的滯回規則，包括park滯回規則、 blakeley滯回規則、 mander滯回規則、張秀琴滯回規則、袁錦根滯回規則以及本文提出的滯回規則等，使其能適用於結構地震反應動力分析。
  7. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  8. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  9. In order to analyze their psychological characteristics comprehensively, the research was carried out on 152 bearing and unboarding children in wenzhou, which adopt group intelligence test for students, eysenck personality questionnaire ( epq ), group thematic test for students ( t - tat ) and symptom checklist 90 ( scl - 90 ). it is shown that these children has developed their unique characteristics

    為了全面了解寄託兒童的心理特點，本研究採用中小學團體智力篩選測驗、艾森克人格問卷（兒童版） 、學生團體主題統覺測驗（ g ? tat ）和癥狀自評量表（ scl ? 90 ）四個量表對溫州市152名寄託兒童和一般兒童進行調查分析，結果表明，寄託兒童已經形成了自己的特徵。
  10. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  11. On the basis of the study of the theory and appraise method on land use in the small towns from home and abroad, this paper at first conducts a deep study on the development and role of the small towns, indicating that its development has sawn an uneven development phrase and becomes a carrier of the enterprises, a pool of surplus laborers, a hub of material exchanges between the rural and urban areas, a base of spiritual civilization, an important way to achieve urbanization. second, it conducts a study on the situation and features and the problems the land use, indicating that the efficiency of the land use is low, which has a direct influence on the development of agriculture and the role of the small towns. and the study of the demand of the land indicates the shortage of land is serious, and the small town must rationally use the land and increases its intensive role and the economical efficiency to meet the demand

    在分析國內外已有關于小城鎮土地利用的理論與評價方法的基礎上，首先對小城鎮在我國的發展、地位和作用進行了深入的分析，判明我國小城鎮發展經歷了一個曲折向上的發展階段，已成為鄉鎮企業的載體，農村剩餘勞動力的蓄水池，城鄉物資交流的樞紐，農村精神文明的基地，是我國城市化的重要途徑；其次，對小城鎮土地資源利用現狀和特徵進行了探討，並對發展小城鎮建設導致的土地利用問題進行了剖析，表明目前我國大多數小城鎮土地效益和規模效益低下，佔用耕地過多，直接影響農業的發展，影響小城鎮的地位和作用；通過小城鎮土地供需分析研究表明，我國土地短缺十分嚴峻，小城鎮土地需求缺口較大，小城鎮必須合理利用現有土地，增強集約功能和土地經濟效益，從而緩解需求壓力；最後，論文通過運用特爾菲法，描述統計分析法、多元統計分析（主成分分析）法和系統分析法中的層次分析法（ ahp ）等一系列方法，結合定性和定量兩方面，從土地質量、土地資源數量與結構、土地經濟效益、環境效益、社會效益等五個方面進行分析，篩選、建立了土地資源利用評價指標體系，在因子評價的基礎上，建立了土地利用綜合評價模型，並給出了評價過程和方法。
  12. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  13. The msftefd hosts the following components that are responsible for accessing and filtering data from tables, word breaking, and stemming

    Msftefd駐留負責從表、斷字和詞干分析中訪問和篩選數據的組件。因此它駐留以下組件：
  14. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  15. The mechanism of fuel saving and emission reduction were discussed in this paper. based on surface chemistry, proper emulsifiers were selected to produce stable emulsified diesel oil, which contains different amount of water. the characters of emulsified fuel such as viscosity, heat value and factors in connect with stability were analyzed

    本文以利用乳化油減少柴油機燃油耗和降低排氣污染為主要目標，先從理論上分析了乳化油節能和降低排放的機理，然後利用表面化學的知識篩選出適當的表面活性劑，復配出高效的復合型乳化劑並用其配製出不同摻水率的穩定的乳化柴油，對乳化油的物性（粘度、熱值等）及影響乳化油穩定性的因素進行了分析。
  16. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  17. In our studies, we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library. the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides. it encodes 431 amino acid

    本研究工作，利用本室構建的核定位信號篩選系統從小鼠胚胎cdna文庫中克隆到一個新的全長cdna片段，分析表明在其1802bp育回軍區大學刃士學位論文的序列中含有一個長1293hp的開放閱讀框，編碼431個氨基酸。
  18. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。
  19. Through rainfall simulating under laboratory, and making slice of sampling, the project researched in the soil crust development, and studied the dynamic rule of erosion factors which were raining and soil erosion factors during soil crust development. in the same time, the research was to find the critical condition of all factors in the process of soil crusting, and to filter the critical factor that could affect soil surface crust

    本文採用人工模擬降雨方法，通過采樣製作土壤切片，觀察分析表土結皮在不同條件下發育的微形態特徵，研究降雨、土壤等侵蝕產沙因子在表土結皮發育過程中的動態響應規律，界定表土結皮發育過程中各因子的臨界條件，篩選有關土壤顆粒組成、結構特徵等物理化學性質對表土結皮形成影響的關鍵因子。
  20. In the new product concept evaluation stage, the filtration method, which considers relative importance and effect of each factor of the multi - scheme, can be mainly adopted. in the new product technical evaluation stage, the ahp can be used to select the best new product design. in the new product launch stage, the methods such as the index evaluation, fuzzy comprehensive analysis, cash flow, finance analysis can be adopted in new product market opportunity select, market sale test evaluation and finance analysis

    在新產品概念開發階段，主要運用多設想（或方案）加權評分法對新產品設想進行篩選；在新產品技術開發階段則主要是運用層次分析法對新產品總體性能進行評價，以選擇總體性能最優方案；在新產品商業開發階段，運用指標評價法、模糊綜合評價法、現金流量表和財務分析表對新產品市場機會、試銷結果、財務分析進行綜合評價，以達到新產品成功上市的目的。
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