篩細胞 的英文怎麼說
中文拼音 [shāixìbāo]
篩細胞
英文
cribrose cell-
High frequency of bud induction was found on ms medium supplemented with 6 - ba the optimum medium for adventitious bud induction was ms + l. 0mg / 1 naa + 0. 5mg / l 6 - ba, and ms + 1. 5mg / l naa + 0. 5mg / l iaa for bud rooting
門)誘導毛狀根體細胞胚再生植株。通過正交實驗篩選出了毛狀根體細胞胚誘導的最佳培養基為: ms 05mg lnaa 0 sing kt 30gil蔗糖。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用,主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library
我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法,獲得了對酵母細胞即無毒性作用,又沒有自激活作用的「誘餌」 ,通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。Immunofluorescence - histochemical changes of the astrocytes in cribriform plate due to high intraocular pressure in rats
大鼠高眼壓后篩板區星形膠質細胞的免疫熒光組織化學變化Results the hyperplastic basal cells were characteristically larger than normal, with oval or spindle nuclei, and formation of double layer - glandlike, solid or cribriform patterns, stained positive for 34 e12
結果顯示增生的基底細胞比正常增大,核呈卵圓形或梭形,常呈雙層腺樣、實體和篩狀結構和34 e12陽性反應特徵。Both intraductal and infiltrating ductal carcinoma are seen here. note the intraductal component in the center with cribriform pattern and prominent microcalcifications. surrounding this are infiltrating carcinoma cells
同時看到導管內癌與浸潤性導管癌。值得注意的是中心處篩型且有明顯微鈣化的導管內癌組成部分。其周圍是浸潤性癌細胞。Cyclosis ( cytoplasmic streaming ) the streaming of cytoplasm in a circular motion around the cell observed in some plants, particularly young sieve tube elements
胞質環流(胞質運動) :在一些植物細胞特別是在幼篩管分子中觀察到的細胞質圍繞細胞的環形流動的現象。Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control
選取單個菌落分別點種到刪平板和md平板,找出在回d上生長正常, w上生長緩慢或不生長的菌落,即陽性菌落,再以陽性菌落分別塗布zeocine含量500ug加, 1000ug砌l的ypd平板,以高濃度的抗生素篩選高拷貝的酵母工程菌,在含500ug ml高濃度抗生素平板上獲得了15個轉化子,取其中5個進行pcr擴增,有3個擴增產物與陽性對照相同,說明此酵母細胞中已含有s hbsag融合片段,其中之一命名為pIsolation and screen of microbial cell flocculent
微生物細胞絮凝物的分離篩選Abstract : selective mechanisms exist in organism and biosphere, which select the cells with less dna injuy and reproduces them. there are two models ; mechanisms 1 and mechanisms 2, represented respectively b bacteria and mechanolytes of human beings. cells with mechanisms 1 ( e. g. bacteria, generative cells, cancer cells ) can reproduce infinitely, while somatic cells can not. there are deep - going reasons behind the two categories of selective mechanisms. the canceration of the cells is a degenerating process from mechanisms 2 to mechanisms 1
摘要生物界存在一類篩選機制,它將dna損傷較小的細胞篩選出來,產生後代.主要有兩類模式機制1和機制2 ,分別以細菌和人的成纖維細胞為代表,那些具有篩選機制1的細胞(如:細菌、生殖細胞、癌細胞)能無限傳代,而體細胞則不能.這兩類篩選機制背後有更深刻的原因,而細胞的癌變正是從機制2退化為機制1的過程。2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent
取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞,再常規融合和篩選,三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。Sp2 / 0 myeloma cells are fused with spleen cells of balb / c mice ( the ratio is 1 : 10 ) immunized with at - by routine method. positive clones are detected by elisa
Sp2 / 0細胞和免疫脾細胞按1 : 10比例融合,融合劑peg分子量為1500 ,陽性克隆篩選採用三步進行。For the purpose of exploiting the affluent resource of traditional chinese medicines, rat pheochromocytoma pc 12 cells were used as a model to screen the neuroactive components from natural products. most of all, the effect of it on inducing the differentiation of pc 12 cells and / or execute the neuroprotective effect challenged by serum - deprivation and oxidative pressure, and its mechanism were studied in this paper
本文以利用我國豐富的天然藥物資源為目的,用大鼠嗜鉻神經瘤細胞pc12作為模型,篩選能模擬ngf的功能誘導pc12細胞分化、對神經細胞有一定保護作用的天然藥物,並對其作用機制進行初步探索。Albuminous cell 1. a vertically elongated parenchyma cell, found in groups in the rays of the secondary phloem in gymnosperms, where they are associated with sieve cells
蛋白質細胞: 1 .在裸子植物的次生韌皮部薄壁組織和韌皮射線中發現的1種垂直伸長的細胞,通常與篩胞相結合。They are common in many situations, for example as gland cells and epidermal cells, and in xylem and phloem parenchyma, where they are concerned with active loading and unloading of vessels and sieve tubes
許多情況下都有傳遞細胞發揮作用,例如:腺細胞和表皮細胞,還有木質部和韌皮部的薄壁細胞,該處導管和篩管的主動裝填和排空都與傳遞細胞有關。The targeted disruption of mouse rbp - j revealed that homozygous null mutants die before 10. 5 days postcoitum ( dpc ) with various abnormalities, including growth retardation and defects in the central nervous system and somites. in order to assess the function of rbp - j, a yeast two - hybrid screening using rbp - j as a bait protein was performed and a nuclear matrix protein, mint was isolated
Sdpc胚胎和人的hela細胞cdna文庫中篩選到一個可以與rbp斗相互作用的新蛋白ram7 ,后發laram7與mmt ( msxz inter肌tingnucleartargetprotein )為同一蛋白。Other modifications include the uneven thickening of guard cells, the sieve plates in phloem, the suberization of endodermal cells, the waterproof coverings of epidermal and cork cells
此外還有其他的一些修飾形式,包括不均勻的加厚保衛細胞和韌皮部中的篩板,內皮細胞的栓化等。Conclusion : the system not only could pick upon the op timum strain on fixed culture medium or the optimum substrate to special strain fleetly but also could be used to evaluate the microbe growth character apace, monitor z ymogen upgrowth in fermentor and determine total number of bacteria in fresh cre amery rapidly
結論:研製的生物傳感器系統可用於菌種和培養基組分的快速篩選、微生物活細胞生長特性的快速評估,還可用於發酵罐中發酵菌的生長監測及新鮮牛奶中雜菌總數的快速測定。分享友人