篩下產物 的英文怎麼說
中文拼音 [shāixiàchǎnwù]
篩下產物
英文
feel-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。This machine is comprised of screen box, vibration source and damper, vibrating frame and screen box are connected with 4 - 12 group soft rubber damp - ers fixed up and down, and the centrifugal force is generated therein, the floating ampli - tude is controlled by the damper, by which, material realizes a process of throw and lami - nation filtration downward, and therefore reach - ing the optimized grading and screening ef - fect
本機由篩箱.振動源及減震器三大部分組成.振動機架與篩箱連接有4 - 12組軟橡膠減震器上下固定，產生離心力，由震器控制浮動振幅，使物料有一個向下拋震和向下分層過濾的過程，從而達到最佳分級過篩的效果The series equipment versatillty, its performance had achieved the international advanced level, is at present is most effective, the practical reliable crushed stone machine, is suitable specially for the manufacture grinding compound, fireproof the matreial, the cement, the quartz sand, the emery, the stove are cut broken glass the power, the copper ore, the concrete aggregate and so on many kinds of, the crisp materials on the control granulated substance machine energy conserva tion 50 %, is in the present world system qranulated substance equipment
物料由機器上部垂直落入高速旋轉的葉輪內，在高速離心力的作用下，與另一部分以傘狀形式分流在葉輪四周的物料產生高速撞擊與粉碎，物料在互相撞擊后，又會在葉輪和機殼之間以物料形成渦流多次的互相撞擊、摩擦而粉碎，從下部直通排出，形成閉路多次循環，由篩分設備控制達到所要求的成品粒度。The fed - in materials move rapidly, so the mlh screens can process more materials per certain size of the cloth than competitors. vibrating screen. vibrating screen. stronger dewatering power is in effect when the mlh screens handle damp materials. vibrating screen
物料從給料機均勻地進入振動篩的進料口，通過振動篩多層篩網產生數種產品和不合格的篩上物振動篩篩下物分別從各自的出口排出。When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。Biodiversity exists among am fungi and is influenced by numerous factors including soil properties and plant species. if am fungi are to be used in sustainable agricultural systems it is necessary to study native am fungi in the target areas and then select efficient isolates that can be applied as inocula in the field to improve crop growth. the objectives of this study were to investigate the germplasm of am fungi, to understand the distribution pattern of am fungi in different ecological conditions such as area, soil factor and host plant, to select isolates effective in nutrient acquisition by the host plant sweet potato, to test their effectiveness under field conditions, and to monitor amf after their introduction into the field
本研究通過調查我國北方部分地區的am真菌資源，研究了am真菌的種群組成及其在空間、土壤利用方式和宿主植物類型等不同環境條件和空間尺度上的分佈規律；在此基礎上，根據它們對甘薯的生長、吸磷效應篩選出高效菌株，在大田條件下研究了am真菌菌絲的分佈特性、代謝活性及其對甘薯產量和品質的影響；並通過分子探針跟蹤調查了引入am真菌在共生體中的發育和表達，以期為菌根真菌的生產應用提供技術支持。The dried powder raw materials will be fed from the top of dry granulator, then enters into flaking machine where becomes flakiness raw material will be made into the needed granule product through the procedures of crushing, processing granule and sieving powder and so on
乾燥后的各種乾粉物料從干法制粒機的頂部進入，經預壓進入軋片機內，在軋片機的雙輥擠壓下；物料變成了片狀、片狀物料經過破碎、整粒、篩粉等過程，得到需要的粒狀產品。After the acet is vaporized, the active substance in water is gotten. and which is vaporized at low temperature. then the crude active substance is purified by column chromatography on sephadex g - 75. after a series of purifications again, we could get some white powder at last. though the active substance is diluted to50 g / ml, the activity is still checkeded - up through phyto phtnora casicileon. the purified active substance is insensitive to heat, resistant to chloroform 、 ethanol and the orhers. in addition, the active substance is sensitive to high ph ( 10 ~ 14 ), but it is not sensitive to low ph ( 1 ~ 5 ). furthermre, when the ph is made to low again, the activity of it ' s comes back
用蒸餾水對菌體稀釋；加入適量吸附樹脂在150rpm 、 28下振蕩吸附4h ， 80 %的丙酮解吸，過濾解吸液得到活性物質的澄清溶液，旋轉蒸發儀旋轉蒸發去處丙酮，經sephadexg - 75分子篩層析得單一活性峰，收集峰值部分樣品液經冷凍乾燥得到淡褐色粉末，該活性物質用丙酮充分洗滌、甲醇-乙醚重結晶獲得略帶微黃的白色粉末，該活性物質50 g / ml仍可對蘇雲金芽孢桿菌hd - 1產生明顯的抑制作用。Two pta - mutants have been selected by using suicide substrate after the mini - tn5 transposon insertion mutagenesis of klebsiella pneumoniae m5al. when used in microaerobic fermentation, the amount of acetate produced by the mutants reduced to less than 50 % of the parent strain, and the yields improved whereas the 1, 3 - propanediol titers and productivities decreased
以klebsiellapneumoniaem5al為出發菌株，用mini - tn5隨機轉座誘變結合自殺性底物篩選的方法得到了兩株產乙酸途徑pta基因缺失突變株xl - 6和xl - 11 ，應用到微氧法發酵中，突變株的乙酸產量為親株的50以下，甘油轉化率有所提高，但1 ， 3 -丙二醇濃度和生產強度有所下降。Through rainfall simulating under laboratory, and making slice of sampling, the project researched in the soil crust development, and studied the dynamic rule of erosion factors which were raining and soil erosion factors during soil crust development. in the same time, the research was to find the critical condition of all factors in the process of soil crusting, and to filter the critical factor that could affect soil surface crust
本文採用人工模擬降雨方法，通過采樣製作土壤切片，觀察分析表土結皮在不同條件下發育的微形態特徵，研究降雨、土壤等侵蝕產沙因子在表土結皮發育過程中的動態響應規律，界定表土結皮發育過程中各因子的臨界條件，篩選有關土壤顆粒組成、結構特徵等物理化學性質對表土結皮形成影響的關鍵因子。Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。This machine consists of bin, vibration chamber, coupling and motor. the vibration chamber is made up of eccentric wheel. rubber soft piece, main shaft and bearing. the adjustable eccentric hammering is transmitted to the centerline of main shaft by motor drive, and produce centrifugal force at unsteady state so as to change the strength of material and form vortex in the sieve. weghted regulator and amplitude can be adjusted as per different material and sieve mesh
本機由料斗、振蕩室、聯軸器、電機組成，振蕩電腦內由偏心輪、橡膠軟體、主軸、軸承組成，可調節的偏心重錘經馬達驅動傳到主軸中心線，在不平衡狀態下，產生離心力，使物料強度改變在篩內形成漩渦，重錘調節器和振幅大小可根據不同物料和篩網進行調節。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。分享友人