篩選主體 的英文怎麼說
中文拼音 [shāixuǎnzhǔtǐ]
篩選主體
英文
filtering agent-
This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用,主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。The results are summarized as follows. solid culture. in preliminary experiments, broomcorn millets ( yielded by the crop panicum miliaceum l. ) and foxtail millets ( yielded by the crop setaria italica l. ) were determined as optimal substrate for solid culture of p. delphacis
主要研究內容和結果分述如下:固體培養篩選確定了以黍米( panicummiliaceuml . ,俗稱黃米)和粟米( setariaitalical . ,俗稱小米)為最適固體培養基質,對飛虱蟲癘霉進行了成功的繁殖試驗。Abstract : selective mechanisms exist in organism and biosphere, which select the cells with less dna injuy and reproduces them. there are two models ; mechanisms 1 and mechanisms 2, represented respectively b bacteria and mechanolytes of human beings. cells with mechanisms 1 ( e. g. bacteria, generative cells, cancer cells ) can reproduce infinitely, while somatic cells can not. there are deep - going reasons behind the two categories of selective mechanisms. the canceration of the cells is a degenerating process from mechanisms 2 to mechanisms 1
摘要生物界存在一類篩選機制,它將dna損傷較小的細胞篩選出來,產生後代.主要有兩類模式機制1和機制2 ,分別以細菌和人的成纖維細胞為代表,那些具有篩選機制1的細胞(如:細菌、生殖細胞、癌細胞)能無限傳代,而體細胞則不能.這兩類篩選機制背後有更深刻的原因,而細胞的癌變正是從機制2退化為機制1的過程。In this paper, genetic transformation systems by agrobacterhim rhizogems was established and optimized and high quality regenerating system was selected. the products of indigotin and indirubin in regenerating plantlets had been detected by hplc. the main studies and results were reported as follows : 1 establishment and selection of hairy root clones taken cotyledons of autotetraploid indigoblue woad as explants and hairy roots induced successfully from them by agrobaterium rhizogenes strains a4 and r1000
本文利用發根農桿菌rit - dna建立並優化了四倍體菘藍的遺傳轉化體系,並對再生植株的主要代謝產物靛藍、靛玉紅進行了含量檢測,篩選了優良無性系,也為實現外源抗蟲、抗病等基因的導入奠定了基礎。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上,根據國內外已發表的ibv基因序列,分別設計特異性引物,應用不同引物進行反轉錄合成cdna ,分片段對ibv的主要結構基因進行pcr擴增,並分別將各個目的片段克隆到puc19載體上,在大腸桿菌dh5中實現目的基因的分子克隆,經藍白斑篩選、限制性內切酶分析、 pcr鑒定,篩選出重組陽性質粒,並對各個目的基因片段進行序列測定,從而獲得ibv主要結構基因全序列。The regeneration system of tobacco was established. the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2. 0mg / l 6 - ba and 0. 3mg / l naa. then the regenerated plants were rooted on ms medium containing 0. 3mg / l naa
植株再生體系的建立採用無菌苗煙草葉片為外植體,以ms為基本培養基,篩選出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以誘導不定芽的分化,並於ms + naa0 . 3mg l的pa碩士學位論文v沉了砂「工隊nk 」主根培養基上生根,獲得完整再生植株。The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease
主題包括:基因、染色體與基因組的結構和功能;來自於基因重組、突變和篩選的生物變異;族群遺傳學;運用遺傳學的方法分析蛋白質的功能,基因的調控和遺傳性疾病。Otoacoustic emissions ( oaes ) exhibits good long - term ' stability for each individual, but there are some evident differences among different individuals
摘要耳聲發射是重要的人體生理聲信號之一,主要用於聽生理研究、聽覺疾病診斷和新生兒聽力篩選方面。In order to analyze their psychological characteristics comprehensively, the research was carried out on 152 bearing and unboarding children in wenzhou, which adopt group intelligence test for students, eysenck personality questionnaire ( epq ), group thematic test for students ( t - tat ) and symptom checklist 90 ( scl - 90 ). it is shown that these children has developed their unique characteristics
為了全面了解寄託兒童的心理特點,本研究採用中小學團體智力篩選測驗、艾森克人格問卷(兒童版) 、學生團體主題統覺測驗( g ? tat )和癥狀自評量表( scl ? 90 )四個量表對溫州市152名寄託兒童和一般兒童進行調查分析,結果表明,寄託兒童已經形成了自己的特徵。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。On the basis of the study of the theory and appraise method on land use in the small towns from home and abroad, this paper at first conducts a deep study on the development and role of the small towns, indicating that its development has sawn an uneven development phrase and becomes a carrier of the enterprises, a pool of surplus laborers, a hub of material exchanges between the rural and urban areas, a base of spiritual civilization, an important way to achieve urbanization. second, it conducts a study on the situation and features and the problems the land use, indicating that the efficiency of the land use is low, which has a direct influence on the development of agriculture and the role of the small towns. and the study of the demand of the land indicates the shortage of land is serious, and the small town must rationally use the land and increases its intensive role and the economical efficiency to meet the demand
在分析國內外已有關于小城鎮土地利用的理論與評價方法的基礎上,首先對小城鎮在我國的發展、地位和作用進行了深入的分析,判明我國小城鎮發展經歷了一個曲折向上的發展階段,已成為鄉鎮企業的載體,農村剩餘勞動力的蓄水池,城鄉物資交流的樞紐,農村精神文明的基地,是我國城市化的重要途徑;其次,對小城鎮土地資源利用現狀和特徵進行了探討,並對發展小城鎮建設導致的土地利用問題進行了剖析,表明目前我國大多數小城鎮土地效益和規模效益低下,佔用耕地過多,直接影響農業的發展,影響小城鎮的地位和作用;通過小城鎮土地供需分析研究表明,我國土地短缺十分嚴峻,小城鎮土地需求缺口較大,小城鎮必須合理利用現有土地,增強集約功能和土地經濟效益,從而緩解需求壓力;最後,論文通過運用特爾菲法,描述統計分析法、多元統計分析(主成分分析)法和系統分析法中的層次分析法( ahp )等一系列方法,結合定性和定量兩方面,從土地質量、土地資源數量與結構、土地經濟效益、環境效益、社會效益等五個方面進行分析,篩選、建立了土地資源利用評價指標體系,在因子評價的基礎上,建立了土地利用綜合評價模型,並給出了評價過程和方法。According to the special shape and operate requirements of the torpedo, the structure and moulding process of the torpedo shell are established, in which the body and overlay block with grooves are moulded by fiber winding, and close moulding respectively with the adhesive by limited stop compression. in the light of the selection of raw material system, composite system with outstanding performance are defined, based on which the mechanical properties are tested., with the overall consideration of structural characters and design demands, the main factors which may have effects on the strength and stability ( including stiffness section dimension, stiffeners space, skin thickness and skin lay angle ) is optimized one by one for the structure design parameters on the base of the fem model of skin, stiffness and layover block by fem. as a result, the prototype with 1 : 1 proportion and its structure and lay optimization design are completed. the moulding technology for polyurethane foam moulding the stiffened shell is obtai ned in terms of the design, manufacture moulding, experimental mould and tooling
依據在研產品特殊的外型結構和使用要求,確定了該型號水雷復合材料殼體的具體結構形式和復合成型工藝路線,即由纖維纏繞工藝成型主體異形件,閉模成型帶通槽外貼塊,用限位加壓工裝實現粘接的工藝路線;通過對原材料體系的篩選,確定了綜合性能較好的復合材料體系,以此為基礎進行了力學性能測試;運用有限元方法,建立了蒙皮、筋條和外貼塊的分析模型,綜合考慮結構特點和設計要求,對影響結構強度與穩定性的主要因素(包括筋截面尺寸、筋間距、蒙皮厚度、蒙皮鋪層角度)分別進行了結構設計參數的優選,最終完成了復合材料異形耐外壓殼體1 : 1樣件和縮比實驗件結構及鋪層優化設計;完成了聚氨酯泡沫胎成型內置加筋殼體的成型工藝技術研究;對縮比件進行外壓性能測試,並給出了應力、應變測試結果。Biodiversity exists among am fungi and is influenced by numerous factors including soil properties and plant species. if am fungi are to be used in sustainable agricultural systems it is necessary to study native am fungi in the target areas and then select efficient isolates that can be applied as inocula in the field to improve crop growth. the objectives of this study were to investigate the germplasm of am fungi, to understand the distribution pattern of am fungi in different ecological conditions such as area, soil factor and host plant, to select isolates effective in nutrient acquisition by the host plant sweet potato, to test their effectiveness under field conditions, and to monitor amf after their introduction into the field
本研究通過調查我國北方部分地區的am真菌資源,研究了am真菌的種群組成及其在空間、土壤利用方式和宿主植物類型等不同環境條件和空間尺度上的分佈規律;在此基礎上,根據它們對甘薯的生長、吸磷效應篩選出高效菌株,在大田條件下研究了am真菌菌絲的分佈特性、代謝活性及其對甘薯產量和品質的影響;並通過分子探針跟蹤調查了引入am真菌在共生體中的發育和表達,以期為菌根真菌的生產應用提供技術支持。Our main products are environmental chambers, on - line reliability evaluation system, burn - in system and lcd processing equipment
經銷產品主要包括各種模擬環境試驗設備、在線快速可靠性測試評估系統、半導體老化篩選設備、液晶熱處理設備。They exist in the selection and diffusion process of technological and organizational changes, and result from the cooperation between the stakeholders and selection
它們出現于技術和組織變遷中的篩選和擴散過程中,是有關主體之間相互合作和篩選過程的結果。In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method
為研究外生菌根真菌本身對重金屬污染的耐性,比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法,以真菌生物量大小和分離難易程度為主要指標,篩選出液體靜置方法為最優方法。The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體,用異硫氰酸胍法提取總rna ,總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時,通過優選擴增體系,使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因,並能定向克隆到載體pgem ? 3z ( amp ~ r )中。用克隆載體轉化宿主大腸桿菌jm109 ,通過篩選獲取陽性克隆子,對陽性克隆子進行酶切與pcr鑒定,並對載體中插入的目的基因進行測序。The intrusion detection system based on artificial immune model simulates negative selection, clonal selection, and memory cells in the human immune system and assigns detection to six kinds of functional agents, which are monitoring agents, decision agents, response agents, communication agents, selection agents and testing agents. though sharing information and interaction with each other, these agents can identify anomalous behavior mode
該系統模擬了人體免疫系統中的負篩選、克隆篩選和記憶細胞等基本工作原理和機制,將入侵檢測的任務分配給監視、決策、響應、通信、篩選和測試等六個功能主體,通過這些主體之間的信息共享、相互協作來識別異常行為模式。In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia
並以已構建的載體ptyb2 - mae為模板,通過pcr擴增出融合基因mae - imein - cbd ,將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。Filters are similar to select queries, but they are used primarily to quickly view a subset of records when you are using a form or datasheet
篩選與選擇查詢類似,但篩選主要用於在您應用窗體或數據表時快速查看記錄子集。分享友人