簡並性引物 的英文怎麼說

中文拼音 [jiǎnbàngxìngyǐnwù]
簡並性引物 英文
degenerated primers
  • 簡 : Ⅰ形容詞（簡單） simple; simplified; brief Ⅱ動詞1 （使簡單; 簡化） simplify 2 [書面語] （選擇人才...
  • 並 : 併名詞1. （山西太原的別稱） another name for taiyuan （in shanxi province）2. （姓氏） a surname
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 引 : Ⅰ動詞1 （牽引; 拉） draw; stretch 2 （引導） lead; guide 3 （離開） leave 4 （伸著） stretch 5 （...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 簡並性 : degeneracy
  1. Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene

    基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌魚masp或其同源基因的一部分序列。
  2. The traditional homogene cloning method was modified on two aspects : first, homo - primers was designed at the sites with minimum degeneracy instead of maximum homology, secondly, some " n " bases were replaced with inosine in degenerate codes, resulting hi the degeneracy reduced from over 1000 times to below 100 times

    改進后的同源引物簡並度從1000倍以上降低至100倍以下，利用rt一pcr成功地擴增出一條630bp的鹽藻cdna片段，該片段與其它物種的烯醇酶基因具有很高的相似性。
  3. All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations

    依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。
  4. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術，以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物，從蠶豆葉片中還擴增到了3個618bp （ 618 - 1 、 618 - 2和618 - 3 ）的cdna片段；用race技術，以618 - 1序列設計的一對基因特異性引物，克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  5. We fully utilized the characteristics of the deficient electron structure ( carbon sp3 and boron ) and easy oxidation introducing - oh on bdd electrode surface to preparation of biosensors, which were used to investigate the electrochemistry of biological molecules and be rapid, sensitive

    利用bdd電極的表面缺電子結構（ sp3雜化碳和摻雜的硼）和易於氧化引入- oh的特點，在氧化或未氧化的bdd電極表面修飾化學/生物物質製成化學/生物傳感器，研究生物分子在電極表面的電化學特性，並實現對生物分子的準確、快速、靈敏、簡便測定。
  6. The theory of property rights are briefly discussed at the beginning. there are varied explanations about the definition of property rights in economics listed here and three common points of the definition of property rights are synthesized : ( 1 ) property rights are exclusive rights and can be equally exchangeable. ( 2 ) property rights are behavioural relations admitted by people who use substance because of its existence. ( 3 ) property rights are plural and a set of rights

    文章首先簡要介紹了產權的基本理論，對產權定義的不同解釋，文中列出了幾種有代表性的看法，並總結出產權定義的三個共同之處： （ 1 ）產權是一種排他性的並且可以平等交易的權利； （ 2 ）產權是由物的存在及關於它們的使用所引起的人們之間相互認可的行為關系； （ 3 ）產權是一個復數，是一組權利束。
  7. Ways and methods of searching information of traditional chinese medicine on internet are described in five aspects, such as the websites of traditional chinese medicine, home pages of traditional chinese medicine colleges, biomedical information networks, medical search engines and directories, and other searching engines and information networks

    摘要本文從五個方面概述了在因特網上查找中醫藥信息資源的途徑，包括從中醫藥學專業網址中查找，從中醫院校的主頁上查找，從生物醫學信息網路中查找，利用醫學搜索引擎與目錄查找，利用通用檢索工具和綜合性網路查找，並簡單介紹了使用方法。
  8. This general formula can describe not only the releasing process of elastic energy in gas reservoir but also the material balance phenomenon caused by the retrograde condensation in gas reservoir and it can be simplified as the material balance equations of condensate gas reservoirs under several special cases, thus providing a effective means for the performance analysis and prediction of condensate gas reservoir development

    該凝析氣藏物質平衡方程通式既能描述氣藏彈性能的釋放過程，又能描述氣藏反凝析引起的物質平衡現象，並可簡化為幾種特例下凝析氣藏的物質平衡方程，為凝析氣藏開發動態分析及預測提供了一種有效的手段。
  9. Engineering drawing has always been labeled as a practical subject. a combination of geometrical, building. mechanical and electrical drawing, it relates between theory and the picture of reality. engineering drawing will provide an accurate and complete ptcture for every object tn terms of shapes and sizes. usually, it is taught using the face - to - face teaching mode even in an odl environment. due to its nature, some students may find difficulty in imagining and interpreting the drawings. however, the availability of sophisticated technology provides the opportunity for the learning of engineering drawing to be enhanced via online. a web - based system for teaching and learning engineering drawing was developed based on a constructivism model. the web - based system is tailored for several topics of engineering drawing such as orthographic projection, sectional view, isometric and oblique drawing at the secondary level. the learning strategy consists of multiple phases beginning with introduction, concept learning, engineering drawing method, application and exercises. during introduction, students will be exposed to an overview of the topic followed by learning of specific concepts. the system provides a learning environment that allows engineering students to view objects from different angles, such as third angle projection and first angle projection as well as views of plans, side and front elevations. after learning about the concepts, students wilt be guided through the various steps in drawing methods for each topic via animations and simulations. learners are able to view any section repeatedly. examples of real application of engineering drawings were also given using graphic, animations and video. to evaluate students understanding, exercises were given at the end of each session

    工程制圖一直被認為是一門實踐性學科，其整合了幾何學、建築、力學、電子制圖等，從而將理論與現實圖像聯系起來，工程制圖能為每個不同形狀、尺寸的物體提供精確的、完整的圖像.通常，即使在開放與遠程教育環境中，工程制圖的教學也是通過面對面的教學模式來進行的.由於其特殊性，一些學習者可能難以想象並解釋這些圖像.然而，尖端的技術使得可以通過在線的方式加強工程制圖的學習.研究者基於建構主義模式開發了一個面向工程制圖教學和學習的網路系統.該系統適用於幾種工程制圖，例如展開圖、刻面圖、等角圖和斜角圖.學習過程包括導論、概念學習、工程制圖方法，以及應用與練習等階段.在導論階段，系統為學習者提供了專題簡介，然後是概念學習階段.系統所提供的學習環境允許工程專業的學生從三維透視、一維透視、平面圖、側立面、正立面等不同角度來觀察物體.經過概念學習階段后，系統將引導學生通過動畫和模擬學習每個專題中制圖方法的不同步驟，學習者也能重復觀察任何剖面.另外，還通過圖像、動畫和視頻等方式展示真實的工程制圖應用案例.最後，為了評價學生的理解能力，在每部分內容後面都附有相關的練習
  10. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
  11. Three special bands as pcr products, obtained by using primer no. l, are reclaimed, and then used to cloning and sequencing. through homologous comparison with data from international nucleotide databank, the band ' s functions have been simply analyzed

    對1號簡並引物擴增出的3條特異性條帶進行了回收、克隆和測序；與國際核苷酸數據庫進行了同源性比較，對特異性片段的功能進行初步的分析和確定。
  12. In this dissertation, the following experiments are conducted : firstly, cloning of hpab - gene, i ) the amino acids sequences of 23 animal - peptide antibiotics were analyzed by multiple sequences alignment method and a pair of degenerated primers were designed according to the consensus sequence derived from mature peptides of some beta peptide antibiotics

    人源肽抗生素hpab -的基因克隆：對動物23種型肽抗生素的氨基酸序列進行對比，選擇部分相似性較高的序列，分析其成熟肽的結構特點，抽提型肽抗生素的一級結構特徵，並以此為基礎設計簡並引物。
  13. Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl

    應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。
  14. This paper introduces the whole process porting and configuring from standard i386 architecture to arm architecture. this paper introduces the embedded system and linux operating system first, obtains the combination - embedded linux, and analyzes the features, advantages and current trends in the world. then introduces the hardware of embedded operating system, design an embedded system that can meet the requirements and improve the abilities of porting and reuse

    本文首先對嵌入式系統、 linux操作系統進行了簡單介紹，然後引出了二者結合的產物? ?嵌入式linux系統，並分析了其特點、優勢和目前國內外的研究情況；而後介紹了嵌入式系統的硬體，從整體上把握一個嵌入式系統使之即滿足應用要求，又能提高軟體的可移植性和可重用性。
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