粒子酶 的英文怎麼說

中文拼音 [lìzi]
粒子酶 英文
particulate enzyme
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 粒子 : grain; granule
  1. The aleurone grains of ungerminated seeds contain hydrolases.

    未萌發種子的糊粉粒含有水解酶。
  2. Aleurone grain ( aleurone body ) a modified vacuole found in the embryo and endosperm of seeds and containing mostly reserve proteins, but also phytic acid and various enzymes associated with mobilization ( digestion ) of these reserves

    糊粉粒（糊粉體） ：在種子胚或胚乳中發現的經過修飾的液泡，裡面包含許多儲藏蛋白，植酸和各種各樣的酶。
  3. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  4. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  7. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  8. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  9. In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate

    將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。
  10. Meanwhile, in order to improve the e. coli with ability of using sugar. we have recombined the vecter of parg25 a which have ability of using sugar with the gene of sucrase length of 7800bp

    同時，為了促進所構建的大腸桿菌具有蔗糖利用能力，我們構建了含有大小為7 . 8kb的蔗糖水解酶基因sacc重組質粒，篩選得到含有兩種基因的重組子，並轉化大腸桿菌，使大腸桿菌獲得蔗糖利用能力。
  11. These help a mitochondrial enzyme called carnitine acetyltransferase to do its job

    這兩種分子有助於被稱作肉堿乙酰轉移酶的線粒體酶發揮作用。
  12. Molecular systematics of partial species belonging to 5 subfamilies of miridae are investigated in this study by using esterase isozyme ( est ) and cytochrome b ( cyt b ) gene in mitochondrial dna as molecular marks

    運用酯酶同工酶和線粒體dna中編碼細胞色素b蛋白質的基因cytb作為分子標記，對盲蝽科miridae的5個亞科的部分種類進行了分子系統學研究。
  13. Electrochemical sensor is quite unique, because it combines the enzyme specificity with the sensitivity and convenience of electroanalytical techniques in a compact form to facilitate analysis

    本文將納米粒子引入酶生物傳感器的制備過程中，主要做了下面兩個部分的工作:第一
  14. In eukaryotic cells, the enzymes and other components of the respiratory electron - transport chain are located in the inner membrane of the mtochondria

    真核細胞中，電子傳遞鏈中的酶與其它成分位於線粒體內膜。
  15. The photosynthetic pigments and accessory pigments are bound to these membranes, which also contain the components of the electron - transport chain and enzymes needed for the light - dependent reactions of photosynthesis

    光合作用色素和一些色素附和物附在基粒的膜上，可能還包含有電子傳遞鏈的組分，以及光合作用光反應所需的酶。
  16. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  17. Nucleoli do not emerge in this process. the number of the organelles increase until secondary spermatocyte stage. mitochondria accumulate together, merging together with lysosomes and golgi bodies at the early spermatid stage, and finally the lamellar structure is formed, which forms the acrosome at last

    在精子發生過程中，線粒體、內質網和核糖體逐漸增多，其中線粒體數目在次級精母細胞階段達到頂峰，並形成線粒體區，精細胞早期核內出現膜性泡結構，同時次級溶酶體與高爾基體大量存在，這些細胞器共同形成片層復合體，並參與頂體的形成。
  18. In this paper, the nanoparticles were used in the fabrication of biosensors. this thesis concerns two research sections : first, a one - step method for fabrication of horseradish peroxidase ( hrp ) biosensor with au nanoparticle ( aunp ) has been developed. the aunp and hrp were simultaneously embedded in silica sol - gel network on gold electrode surface in the presence of cysteine

    用溶膠凝膠（ sol - gel ）包裹納米金粒子（ aunp ）和辣根過氧化酶（ hrp ） ，結合納米技術、溶膠凝膠和自組裝技術，既保持了納米粒子對電極的增敏作用，又大大延長了電極的使用壽命。
  19. In this study, it has been put forward that taking reactive nanometer magnetic fe304 particles as magnetic nucleus, and the copolymer of styrene ( st ) ? crylic acid ( aa ) as macromolecular shell, we could synthesize, magnetic polymer composite microspheres containing carboxyl groups on their surface, then microspheres are activated by thionylchloride, the surface of such magnetic composite microspheres thus produced had reactive acid chloride groups which then react with the free amino groups of the free soluble enzymes to give peptide bonds ( ? o ? h ?,

    本研究首次提出了以納米級磁性fe _ 3o _ 4粒子為核心，苯乙烯（ st ） ?丙烯酸（ aa ）共聚物為高分子殼層，合成了表面帶羧基的磁性高分子復合微球，然後將這種微球用二氯亞碸進行活化處理，在其表面形成了反應性酰氯基團，該基團可以與游離酶的氨基形成肽鍵，從而將游離酶固定化。
  20. In the microemulsions - mediated methods, the nanosize water droplets show thermodynamically stabilization by the means of the surfactants ( sometimes and cosurfactants ) films, that serve as nanosize test tubes, thus limiting particles growth and minimizing particle aggregation. the technique have been applied in some fields including inorganic nanoparticles synthesis, organic polymerization and enzyme catalyst activitization

    在反相微乳液中，由於表面活性劑和助表面活性劑的作用，提供了一個熱力學穩定的納米尺度的水核空間，該水核空間作為可以調節的模版（又稱為智能微反應器） ，對于合成各種無機納米粒子、有機物的聚合以及增加酶的活性都已經引起了廣泛的注意。
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