糖基化位點 的英文怎麼說
中文拼音 [tángjīhuàwèidiǎn]
糖基化位點
英文
glycosylation site-
The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明, pcr擴增產物中包含有完整的phya基因,該基因全長1506bp ,其中包含一段長102bp的內含子,該內含子具有真菌植酸酶基因內含子的特徵保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸,理論分子量為51 . 37kda ,其上有13個潛在的n -糖基化位點, n端19個氨基酸為信號肽序列,植酸酶活性位點序列( crvtfaqvlsrhgaryptdskgk )位於氨基酸序列的+ 71 + 93 。And amino acids of strain mrv between antigenic site and glycosylation site is different. to some extent, it will decide and change the antigen of mrv
Mrv的抗原部位和糖基化位點氨基酸均有變異,這種變異可能在不同程度上會改變毒株的抗原性。The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored
(二)寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析,在檢測的9個酶系統中,共檢測到11個酶位點:天冬氨酸轉氨酶( l個位點, 2個等位基因) ,堿性磷酸酶( 2個位點, a加了和a加2各有2個等位基因) , r澱粉酶( l個位點, 2個等位基因) ,醋酶( 2個位點, es巧和est7各有2個等位基因) ,蘋果酸脫氫酶( l個位點, 3個等位基因) ,蘋果酸酶( l個位點, 2個等位基因) ,乳酸脫氫酶( l個位點, 4個等位基因) ,磷酸葡萄糖轉氨酶( l個位點, 3個等位基因) ; a澱粉酶為單態。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點,包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等,拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能,而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( eMany glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid
Gpi化前體蛋白在依附於膜的核糖體上合成,當其易位穿過內質網( er )膜后,被gpi :蛋白質轉酰胺基酶( gpit )識別, gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame
人類gnt - v由741個氨基酸組成,有6個潛在的n -糖基化位點,基因定位於染色體2q21 ,含有17個外顯子,其開放閱讀框架由外顯子2 - 17進行編碼。It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein
跨膜區域( tmd )將它分為胞內和胞外兩個部分,它的胞外有13個潛在的糖基化位點, 20個蛋白激酶c的磷酸化位點, 28個酪蛋白激酶的磷酸化位點, 4個酪氨酸酶的磷酸化位點, 15個豆蔻(十四烷基)酰化位點;它的胞內有1個整合蛋白( integrin )識別位點。The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites, four conserved cysteine residues, two out - of - frame atg initiation codons in the 5 " untranslated region, and a signal peptide consisting of 66 amino acids. when it was compared with the published sequence of chil - 15 cdna, 7 mutant sites were found, and 5 amino acids were changed in predicted amino acids, which indicated that chil - 15 may be polymorphic
結果顯示,本研究所用白來航雞il - 15cdna5 』非編碼區有兩個框外atg起始密碼子,開放閱讀框由564bp組成,編碼187個氨基酸,其中n末端信號肽含有66個氨基酸殘基,在第48 、 149和166位的天冬酰胺殘基上有三個潛在的n -糖基化位點。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 )為材料分離克隆到一個細胞色素p450基因,命名為bccyp86mf5 , cdna全長1854bp ,含1575bp的完整開放閱讀框,編碼524個氨基酸,其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ;堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ;二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域,半胱氨酸亞鐵血紅素配體信號區等, -螺旋和-折疊分別佔47 . 7 、 45 . 0 ;與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ,與擬南芥cyp86c4的達到85 . 9 。Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co - cultured in vitro to construct tet. lt was frozen in liquid nitrogen with four kinds of cpa for 2 months. post - thawed quickly and transplanted into hind limbs of nude mice, and repaired the defects of achilles tendon. after 2, 4, 6, 8, 12 weeks, the morphological, histological, ultrastructure, short tandem repeat loci and immunohistochemistry examination were detected, and biomechanical strength of tet were examined. result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice. in the group of dmso + raffmose + kh2o4, vacuole in mitochondrion degraded i tendon cell ranged in order, abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed. c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature
組織工程肌腱制備完成後在四種抗凍劑保護下液氮凍存2月;快速復溫后植入裸鼠以修復跟腱缺損, 2 、 4 、 6 、 8 、 12周后取出,觀察形態學、組織學、電鏡和免疫組織化學變化,短串聯重復位點檢測和生物力學變化。結果實驗組組織工程肌腱體內植入12周后仍有肌腱細胞存活並分泌型膠原;隨著時間延長, 10二甲基亞碸( dmso ) +棉子糖( 30mmol l ) + kh _ 2po _ 4 ( 25mmol l )組線粒體空泡減少,肌腱細胞排列整齊,膠原纖維增粗並連接,抗拉強度增高。Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids
Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵,包括36個氨基酸組成的疏水信號膚,一個cub ( clr / cls , anembryonicseaurehinproteinuegf , andbonemorphogeneticproteinl )結構域和一個胰酶樣絲氨酸蛋白酶結構域( t汀psin一likeserineproteasedomain )和幾個保守的糖基化位點等,沒有發現有跨膜區的存在。According to the deduced amino acid of g gene, there are two glycosylation sites in strain 119 and gxbm at position 37 and 319. there is few change in important neutralizing antigenic epitope and in some antigenic sites related with pathogenicity
119和gxbm株g基因推定氨基酸均有37 、 319兩個糖基化位點,重要的抗原位點及致病性相關位點基本沒有變化。The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain
序列分析和二級結構預測表明, f _ ( 48 ) e _ ( 9 )株f蛋白含有3個分別由25個氨基酸組成的疏水區,存在6個潛在的糖基化位點, 13個半胱氨酸殘基,裂解位點區域的氨基酸序列為rrqrrf ,說明f _ ( 48 ) e _ 9株是一株典型的強毒株。Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp. it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level. this gene is a membrane protein which has one signal peptide, seven transmembrane helices, three n - glycosylation sites and one o - glycosylation site
序列分析表明,該基因的cdna全長為1398bp ,開放閱讀框為1095bp ,編碼一個364個氨基酸的多肽,與番茄和豌豆中的該家族基因分別具有71和69的氨基酸同源性,是一種膜蛋白,具有1個信號肽序列, 7個跨膜螺旋, 3個n ?糖基化位點和1個o ?糖基化位點,分子量大約為39 . 174kd ,等電點為8 . 07 。分享友人