純合基因對 的英文怎麼說

中文拼音 [chúngějīyīnduì]
純合基因對 英文
homozygous gene pair
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 對 : Ⅰ動詞1 （回答） answer; reply 2 （對待; 對付） treat; cope with; counter 3 （朝; 向; 面對） be tr...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  3. It was firstly reported in china. the third part rthe capsid protein vp | gene was expressed. 810 bp fragment was inserted into vector pgex - 4tl, and then transformed into e. coli bl21

    這是國內首次對aev基因組全序列的分析；第三部分： aev外殼蛋白vp _ 1基因在大腸桿菌中的融合表達、純化及活性研究。
  4. The thesis is based on income question, forest coverage rate and grop production. to beging with, date envelopment analysis is proved that it can be applicated into grain for green in shan - bei district, and then in view of the fact, seven esential factors which have influence on the project are found out : expense of dam, expense of crop, expense of cash tree, expense of defense tree, other economic crop, expense of grass and graziery ; and the output factors include : income of gdp, pure income per captia, the area of decreasing land loss, graziery income, crop production, income of tree, the totle income of economic crop. after the date of each facts are puted into dea model, unefficiencial decision making units ( dmu ) found. the data that are got through adjusting unefficiencial dmus dy dea can offer guide in shanbei district upgrading of an industrial structure. at the same time, taking into account some possible problems in or after grain for green in shan - bei district, the thesis bring out some propesal to improve the circ umstance, enhance the life level and put the relation of population, resource and circumstance into a healthy orbit

    因此，加快退耕還林還草，調整土地利用結構和產業結構，已成為實現山川秀美工程和可持續發展戰略的必經之路。論文以解決陜北地區的收入問題，植被問題以及糧食產量問題為出發點，首先從理論上分析了包絡分析方法在陜北地區退耕還林（草）中運用的可行性，然後結合陜北地區目前的實際情況，綜合分析出影響陜北地區退耕還林（草）的關鍵的七個因素作為輸入指標：水利水保設施投入，農作物投入，經濟林種支出，防護林支出，其它經濟作物，草類支出，畜牧業投入；以退耕還林所要解決的最終問題作為輸出指標： gdp收入、農民人均純收入、水土流失減少量、牧業總產值、糧食產量、林業總產值、其他經濟作物總產值。將各指標所對應的數據代入模型后，通過分析求解得出非有效的決策單元，再運用包絡分析方法的一些基本原理對非有效的決策單元進行調整，由此所得的數據對陜北地區的實際投入具有很好的指導意義。
  5. Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function

    進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。
  6. Abstract : the main characters and seed set of bc1 ， bc2 ， bc1f2 from ms line back - crossing with the pollen of hybrid rice were studied. the appearance of a large number of self fertile plants in ms line was analyzed. the effect of introgression of restoring genes on ms line purity and hybrid yield in indica and japonica rice was discussed. it was pointed out that “ iso - cytoplasm restorer line ” was seriously reducing the purity of ms line and that hybrid rice was unsuitable for isolation zone in the seed production fields

    文摘:研究了雜交稻花粉對母本不育系回交後代群體bc1 、 bc2 、 bc1f2的主要農藝性狀和育性表現，分析了不育系中出現大量自交結實株的原因，討論了恢復基因滲入對秈、粳不育系純度和雜交稻產量的影響，指出「同質恢」對降低不育系純度的嚴重性和雜交稻作制種田隔離區的不合理性。
  7. Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions

    本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。
  8. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  9. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  10. In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method

    為了選育新類型玉米雄性核不育系，提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略，同時應用分期播種的方法為作物提供不同的生長和發育的環境條件，以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明，春播環境下可鑒定和選擇玉米的不育性，夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性，結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性，為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇，能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法，使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系
  11. Their frequencies of gus expression in pollens were 97. 755 % 0. 800 % and 96. 556 % 0. 600 % respectively. to verify these results, the self - pollinated progenies of these two putative homozygotes were examined in t2 by histochenmical gus staining of vegetative tissues and pcr analysis using specific primers

    （ 2 ）通過對轉基因t1代的花粉gus基因表達檢測，快速鑒定和篩選得到了番茄純合植株，加速了純合體篩選的進程，證明此方法可靠，而且不會造成大量寶貴材料的浪費。
  12. Ddm use region info to describe the supply and demand relation among simulation entities. confirm the aggregate which entities are interested in by region matching. therefore region matching is the basic of data filtering. this paper discusses detailedly the complexity of region matching process from the point of view of the pure arithmetic and develops a rapid matching arithmetic according to the characteristic of region in ieee1516

    Ddm使用區域信息描述實體間的數據供求關系，通過區域匹配確定實體的感興趣集合，因此區域匹配是實現數據過濾的基礎，區域信息匹配計算的復雜度是影響過濾開銷的一個關鍵因素。文中從純演算法的角度對區域匹配過程的復雜度作了詳細的分析，並以ieee1516標準為基礎，結合其中區域的特點，提出了一種單維步進的區域匹配演算法。
  13. Chapter two, sometimes is the common topic to civil law scholars, but the studying products fall short of depth and width, so this chapter is governed by the historic overview and comparative method, under the base of examining the development and vicissitude of roman trust notion, probing into why the civil law did n ' t develop and shape the institution of trusts like anglo - saxon jurisdictions, meantime collecting and analyzing the various experiences and lessons from a number of civil law countries, not only including mixed - system jurisdictions ( scotland, quebec ) influenced by common law tradition far - reachingly, but also including the various effects of the trust law codified by pure civil law countries ( liechtenstein, japan, latin american countries ), and the developments of domestic trust in holland and italy under the rectification of the trust hague convention, ad hoc, including the experiences and lessons of non - recognition trust countries ( germany, france, switzerland ) that they had been penetrating into the notions in civil codes, all is intended to " portrayed " the competitive panorama between civil

    第二章「信託觀念與民法傳統的沖突和融合」 ：這一主題一直是民法學者探討的核心問題，本部分採取了歷史考察的方法和比較分析的方法，在考察羅馬法中的信託觀念的發展和變遷的基礎上，探討了為什麼民法傳統沒有發展出如普通法系一樣的信託的若干原因，同時一廣泛地涉獵了民法法系各國繼受和發展信託觀念的不同的經驗和教訓，不僅包括深受普通法系影響的混合法系（蘇格蘭和魁北克）在民法傳統的物權法匡架中發展信託的獨特經驗，而且考察了純粹民法傳統的國家（列支敦斯登、日本和拉丁美洲國家以法典繼受信託的不同的效果，以及在1984年《關于信託的法律適用和承認的公約卜v發展國內信託例證?一荷蘭和義大利，更包括了對于沒有接受普通法系信託而在民法典中挖掘信託觀念的德國、法國和瑞十的經驗和問題，並意圖勾畫民法傳統中的信託與普通法系的信託相競爭的全景，介刑」析眾多經驗和教訓i的基礎上為我國《信託法》的發展提供借鑒。
  14. This requires both parents to be homozygous for same alleles in question

    對于相同的等位基因來說，父母本的等位基因都要是純合的。
  15. On the hh signal receiving cells, the function of hspg in hhn movement might be that it competes with ptc for binding of hhn, following the releasing from the disp, hhn binds to the hspg to prevent the hhn from being captured by the ptc, hence facilitate it ' s transfer to the more distantly located cells

    進一步結合運用flp frt系統，用缺損gfp蛋白的表達來標記純合的oxt突變基因克隆，針對眼睛成蟲盤的克隆分析結果表明，在oxt突變克隆中， hh下游的以蛋白的表達區域完全消失。
  16. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  17. The sample composition was analyzed by means of tg and x - ray diffraction. the following conclusions can be drawn from the experiments : given conditions, the sample powder that has the same xrd data as the standard fetioa can be gotten when use any method mentioned above ; the key in synthesis is to avoid the oxidation of fe2 +, so the whole process should performed under vacuum or neutral atmosphere, samples used by this study were prepared under nitrogen atmosphere ; the heat - treated time and temperature have some influence on the sample powder, results show that the proper time and temperature is 120 minutes and 1100 respectively

    研究結果表明：在合適的反應條件下，用上述三種合成方法都能得到xrd數據與標準fetio _ 3物相基本吻合的粉體；合成反應需要克服的主要問題是防止體系中fe ~ （ 2 + ）的氧化，因此，整個反應必須在真空或者非氧化性氣體保護下進行，本研究需要的樣品是在高純從保護下合成的；煅燒的時間和溫度對最終的產物也有一定的影響，研究發現：合理的煅燒時間和溫度分別為120分鐘和1100 。
  18. Under the horizontal earthquake action and wind force, aim at " the pure frame structure with rectangle columns ", " the pure frame structure with special - shaped columns ", " the frame - truss structure with special - shaped columns " and " frame - shear wall structure with special - shaped columns ", earthquake action analysis was done by the spacial finite element method through the changes structural parameter. analyzing systematically " structure vibration mode ", " vibration period ", " structure lateral rigidity ", " seismic action force ", " seismic response force ", " floor seismic shear force ", " lateral horizontal displacement of structure " and " members internal force ". results indicate : ( 1 ) based on equal area, the special - shaped columns replaced the rectangle columns, the structural lateral rigidity enlarges, the lateral displacement minishes obviously, the earthquake response increase slightly, the biggest increasing amount of frame columns axis - compress ratio is smaller than 0. 08. the whole aseismic performance of structure has improved ; ( 2 ) when the section ' s length and section ' s thickness ratio of special - shaped columns is smaller than 3. 6, the structure benefits to resist seismic action ; ( 3 ) the angle of horizontal seismic action with the whole coordinate is 0 degree, structure earthquake response is bigger, belonging to a control factor of structure aseismic design ; ( 4 ) the frame - truss structure with special - shaped columns and the " a " - brace has the biggest lateral rigidity ; ( 5 ) the frame - shear wall structure with special - shaped columns have bigger lateral rigidity and smaller displacement, members internal force enlarged just rightly, have much superiority of resisting seismic action ; ( 6 ) in the higher seismic fortification criterion region ( 8 degree of seismic fortification intensity ), aseismic disadvantageous building site ( iii type site ), adopting special - shaped columns structure system, should reduce possibly the building ' s self - weight in order to reduce the earthquake response ; ( 7 ) the response spectrum method of computing seismic response and the time - history analysis method have similar analysis result

    西安理工大學碩士學位論文在水平地震作用下，並考慮風荷載組合，分別對「矩形柱純框架結構」 、 「異型柱純框架結構」 、 「異型柱框一析架結構」及「異型柱框-剪結構」等四種結構體系，通過改變結構參數，運用空間有限元方法，進行地震作用計算。系統地分析研究「結構振型」 、 「振動周期」 、 「結構側向剛度」 、 「結構地震反應力」 、 「樓層地震剪力」 、 「結構側向位移」 、 「層間位移角」 ，以及「構件內力」 。結果表明:在等面積原則下，異型柱代換矩形柱后，結構剛度增大，側向位移明顯減小，地震反應力略有增加，框架柱軸壓比最大增幅小於8 % ，結構整體抗震性能有所提高;當異型柱肢長肢厚比小於等於3 . 6時，結構有利於抗震;水平地震作用力與整體坐標夾角為0度時，結構地震作用效應較大，屬結構設計的控制因素之一;異型柱框一析架結構採用「人」字斜撐，側向剛度大於「八」字斜撐和「人一八」字混合斜撐;異型柱框一剪結構側向剛度大、位移小，構件內力增大適中，是一種抗震性能優越的結構體系:在抗震設防烈度較高地區（ 8度）和抗震不利的建築場地（ m類場地） ，採用異型柱結構體系時，應盡可能減輕結構自重，降低地震作用力;分別採用「振型分解反應譜」法和「時程分析」法進行地震作用計算，兩種方法所得結果基本一致。
  19. These suggest the bsp proteins and its homologous proteins are ubiquitous in mammals and must have some physical significances. in our research, to find and study the important role of bsp proteinsand their related proteins in the development of zygotes and to find the new gene related to bsp, we cloned a cdna gene sequence related to bsp proteins and make it express in eukaratic cells. the fusion protein acquired are purified are used to deduce its biological functions

    而在我們的實驗中為了發現和研究bsp及其相關蛋白在受精卵的發育中的重要作用，尋找bsp相關的新基因，克隆了一個與bsp蛋白相關基因的山一na序列並將該基因編碼的蛋白質在原核細胞中進行了表達和純化，將純化后的融合蛋白作了一些生物學功能方面的初步工作，發現新蛋白對tpk有一定的抑制作用，而tpk是細胞生長和代謝中十分重要的關鍵酶，這對于進一步研究新蛋白的生物學功能具有十分重要的意義。
  20. Based on the retrospect and review of existent literature, spatial monopolization and rival strategy are accepted but the angle discussing the effect that product differentiation works on cooperation will be changed from producer. by re - constructing function of consumer utility and introducing in consumer preference, the degree of product differentiation may be denoted by the degree of consumer preference. the influence that product differentiation works on critical discount factor d can be obtained by introducing in one - shot price game and repeated game, from which we can learn the influence that product differentiation works on cooperation

    圍繞bertrand悖論的所展開的爭論，為進一步展開對這一問題的分析提供了豐富的工具和背景知識，在對已有的理論文獻加以回顧引述的基礎上，保留原有的空間壟斷概念和競爭策略的同時，改變單純從生產者角度來論述產品差異度對企業合作的影響，重新構造消費者效用函數，引入消費者偏好，以消費者的偏好的大小來表示產品之間的差異度，通過單時期和無限期博弈模型來論證產品差異度（消費者偏好程度之比）對臨界折現因子的影響，進而論證其對企業間進行合作所產生影響；效用函數的引入使得價格和運輸成本不再是決定消費者購買的唯一因素，消費者對產品的選擇不完全取決于消費者的位置，這會導致企業間定價和市場份額的非對稱性變化。
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