純培養物 的英文怎麼說
中文拼音 [chúnpéiyǎngwù]
純培養物
英文
pure culture- 純 : 形容詞1 (純凈; 不含雜質) pure; unmixed 2 (純粹; 單純) simple; pure and simple 3 (純熟) skil...
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
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The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria
二、土壤微生物總dna的提取和純化方法研究為了採用不依賴于培養的16srdna分析的方法研究有機磷農藥長期污染對土壤微生物群落結構的影響,建立了從土壤中提取總dna的方法,並通過改進使適合於對革蘭氏陽性菌的提取。In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物,選擇大熊貓原代病料和病毒各代細胞培養物,經套式( nested ) rt一pcr擴增,可得到一與設計值5巧bp相符的dna片段,經bst一xl ( 590 , 1110 )酶切鑒定,證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段,純化,送生物公司測序。The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination
本實驗將sod2基因構建到植物表達載體prok中,導入農桿菌后,進行植物遺傳轉化,實現其在擬南芥中過量表達,在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系,自交一代獲得足夠的純和轉基因種子后,對其進行了分子生物學的驗證及生理指標的檢驗。The axenic culture of rusts has been achieved on various defined media.
在各種規定的培養基上可得到銹菌的單體純凈培養物。Materials and methods in our study, first the e. coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis. the plasmids dna extraction were processed 37 overnight by the restrictive endo - incisase bam h i and xho i. the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec - trophoresis
同預想結果基本吻合,大規模培養后純化得到的融合蛋白通過sds page顯示在52kda處有一條帶,而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶,其中一條為gst ,另一條為hbrp ,基本符合實驗結果; hbrp對tpk活性的抑制呈劑量依賴性。So the preparation of a pure culture involves the isolation of a given microorganism from a mixed natural microbial population.
因此純培養制備要從混合的天然微生物群中分離出特定的微生物。In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method
為研究外生菌根真菌本身對重金屬污染的耐性,比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法,以真菌生物量大小和分離難易程度為主要指標,篩選出液體靜置方法為最優方法。These are main problems of eutrophication. at first we select algae which grow under different level of nutrition ( or different development phase in the process of eutrophication ), domesticate, selecte seed, and culture algae at the lab, and then we obtain dependence of each algae on nutrition and understand information of eco - breadth of nutrition of supplied alga through pure culture under different concentration of nutrition. we find out resource utilization, competition between interspecies and confirm the quantity - effec t relationship between succession of species and nutrition through mixed culture
本文首先選取在不同營養環境下(或富營養化過程的不同發展階段)生長的藻類物種,在實驗室內進行馴化、選種與純化培養;然後通過不同營養水平的純藻培養實驗,獲取不同藻種生長過程對營養物質的依賴行為,從而可以了解每一供試藻種的營養物生態幅信息;再通過對應培養濃度的混合培養試驗,以期揭示生物種群間的資源利用與競爭行為,並可進一步確定生物種群的演替與環境營養物之間的量效關系。The content of this research include : screening the microorganism that can produce bioflocculant from the microorganism sample, select the best microorganism to optimize its culture condition that can increase the flocculating activity to the utmost ; in order to see about his flocculating activity, then using the bioflocculant flocculate the kaolin suspension and compare the flocculating results with the pac hpam ; on the basis of the bioflocculant has excellence flocculating activity, distill and purify the bioflocculant from the culture mediunu give qualitative analysis of its ingredient and reach its flocculating mechanism. the studies include the enrichment culture of the microorganisms that can produce bioflocculant and their screening suggest that they are broad exist in the soil and active sludge
本課題的研究內容包括:從微生物樣品來源中篩選出微生物絮凝劑;從中挑選具有優良絮凝活性的微生物絮凝劑產生菌,對其產微生物絮凝劑的培養條件進行優化以提高其產絮凝劑的絮凝活性;用該絮凝劑絮凝高嶺土懸液,並與聚合鋁( pac )和水解聚丙烯酰胺( hpam )進行比較,以考察其對廢水的絮凝效果;在具有優良絮凝活性的基礎上,將該絮凝劑提取和純化,對其進行分析研究,並對其絮凝機理進行探討。For revealing the mechanism of low bacterial biomass in the lake, limiting factors for bacterial growth were studied with pure cultural and natural cultural tests
為了探明西湖水體中細菌生物量低的原因,通過添加不同營養鹽類的純種和自然培養試驗,對異養細菌生長的限制因子進行了研究。In the process of study for the extracellular cam, the research in our lab indicates that cambps also possibly exist in cell wall area. for the first time, an extracellular ca ~ ( 2 + ) - dependent cambp ( named : ecbp21 ) was purified from 0. 1mol / l cacl _ ( 2 ) extracts of angelica dahurica suspension - cultured cells ( tang et al. 1996 )
我室在對植物細胞外cam的研究過程中,發現細胞外還可能存在cambps ,並首次從白芷懸浮培養細胞外純化了一種依賴ca ~ ( 2 + )的、分子量約為21kd的細胞外cambp ( tang等, 1996 ) (定名為: ecbp21 ) 。The products cover the fields of the study of molecule biology, expression of protein, from cell culture to layer purification, from antigen antibody, the whole set reagent kit to the microorganism culture and the research of theactivity of the biology
其產品覆蓋了生命領域從分子生物學研究、蛋白表達、細胞培養到層析純化,從抗原抗體、成套試劑盒到微生物培養及其生物活性研究等相關試劑幾近所有過程。The ammonia - oxidizers were increased from 2. 13 106 / g mlss to 6. 28 108 / g mlss, while the nitrite - oxidizers were increased from 2. 13 103 / g mlss to 6. 28 106 / g mlss. after a month, most of the heteotrophic bacteria were washed out from the system. they nitrifying bacteria were prevalent in the enrichment system and were around 99 % in total bacteria
選取富含硝化細菌的活性污泥作為富集培養的對象,採用純無機培養基對硝化細菌進行定向富集培養,能在較短的時間內得到大量硝化細菌富集培養物,硝化細菌數約占總菌數的99以上。The molecular approach to detecting peptide hormones using cdna probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them
採用cdna探子來測定肽激素的這一分子生物學方法同時也應該比免疫學的方法速度來得快,因為對于免疫的方法來說,需耗費好幾年枯燥的提純進程,方能將肽素分離了出來,然後再培養出針對它們的抗血清。The white is pure oringial liquid anne a series is french of bolanduo protect the skin research center again on leading the hairdressing item, the item is various original liquid cell live the body is to be really connected the supply by the bio - chemical graduate school development of switzerland a live composition is up to 80 % - 95 %, having to very and quickly show the effect, all products according to the standard microor gansim examinatoin of talllest pharmacy in country of france, strict quality management, assurance the qualityis prtfect
白純原液瓶系列是法國博蘭朵護膚研究中心又一領先美容項目,項目各種原液細胞活體是由瑞士生化研究所培養直接供應,安瓶活性成分高達80 % - 95 % ,具有極快顯著效用,所有產品都依據法國最高藥學標準微生物檢驗,嚴格品質管理、保證品質完美。分享友人