純時后因子 的英文怎麼說

中文拼音 [chúnshíhòuyīnzi]
純時后因子 英文
pure time-delay factor
  • 純 : 形容詞1 （純凈; 不含雜質） pure; unmixed 2 （純粹; 單純） simple; pure and simple 3 （純熟） skil...
  • 時 : shí］Ⅰ名1 （比較長的一段時間）time; times; days:當時at that time; in those days; 古時 ancient tim...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  1. The simple story is in the world of the animation is an absolute main factor, a complicated story details turns and twists confusing link for see an animation of the audience is a tremendous burden, the market need that is in present the top interest has already replaced a traditional story to spread chen, at this interest in command age how expressed a plot to become the most important topic with the simple story, very clearly, advertise from the film of the way can feel what kind of the topic just is the need of the consumer, passed by high cost to create greatly of the advertisement mode has already not been reseen this several years, take but the generation is a film to have many interests much play, this be the typical simple story description complicated plot, give examples to say : the movie “ shrike ” is a very typical example, pure of save a princess of plot, continuously of pound at audience by the plot of " be contrary with traditional thinking " of thinking mode, i still remember at that time see that slice through behind appear details of hear the public discussion interest continuously, but hear half personal say this drama of story have much good, in fact such manuscript was the best animation manuscript, letting the audience acquire they want of plot but don ' t be pack with complicated story, this just is the animation dramatize of the tallest state, because of would like to spend money to enter into theater or spend money to purchase dvd consumer, all is want to open happy heart ' s appreciating film bear, have no burden, also be spend money to buy happy, want to remind specially of " the writing animation manuscript not is write to personal satisfy own creations desire but is face all appreciate of crowd "

    在動畫的世界里簡單的故事是絕對的要素，一個復雜的故事情節曲折迷離的環節對于看動畫的觀眾是一項極大的負擔，在現今的市場需求上趣味已經取代了傳統的故事鋪陳，在這個趣味掛帥的年代怎麼樣用簡單的故事來表現劇情變成了最重要的課題，很明確地，從影片廣告的方式可以感受到什麼樣的主題才是消費者的需求，過去高成本大製作的廣告模式這幾年已不復見，取而代之的是影片有多趣味多好玩，這就是典型的簡單故事描述復雜的劇情，舉例來說：電影史瑞克就是一個很典型的例子，單純的救公主的劇情，不斷的以「與傳統思維相反」的劇情沖擊著觀眾的思考模式，我還記得當時看完該片后出場不斷的聽到眾人討論趣味的情節，但是卻沒有聽到半個人說這部戲的故事有多棒，其實這樣的劇本就是最好的動畫劇本，讓觀眾獲得他們想要的劇情卻不以復雜的故事包裝，這才是動畫編劇的最高境界，因為願意花錢進到戲院或是花錢購買dvd的消費者，都是想開開心心的觀賞影片沒有負擔、沒有包袱，也就是花錢買開心，特別要提醒的「寫作動畫劇本並不是寫給個人滿足自己的創作慾望而是面對所有欣賞的人群」 。
  2. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  3. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
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