細胞分離因子 的英文怎麼說

中文拼音 [xìbāofēnlíyīnzi]
細胞分離因子 英文
cell detaching factor
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 離 : Ⅰ動詞1 （離開） leave; part from; be away from; separate 2 （背離） go against 3 （缺少） dispens...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. According to medical research, when vitamin b12 enters the body, it forms a compound with an intrinsic factor secreted by the parietal cells large cells of the peptic glands on the gastric mucosa mucous membrane of the stomach, before being absorbed by receptors in the ileum lower part of the small intestine in the presence of calcium ions

    在維他命b12的吸收方面，根據醫學報導，維他命b12在吃進人體后，首先會在胃部，和胃壁細胞parietal cells分泌的一種蛋白質內因子intrinsic factor結合，形成復合物后，再由小腸段中的回腸ileum吸收。在回腸之接受體receptors吸收時，需要有鈣離子之存在。
  2. ( 3 ) isolation and culture of human primordial germ cells ( pgcs ). human pgcs collected from gonadal ridges and mesenteries were grown on mouse feeder layers in the presence of human recombinant leukemia inhibitory factor ( lif ), human recombinant basic fibroblast growth factor, and forskolin as described previously. initially, pgcs were visualized by alkaline phosphatase activity staining

    （ 3 ）人類pgcs的分離和培養從4 10周齡藥物流產胚胎的生殖嵴和腸系膜組織中分離原始生殖細胞（ primordialgermcells ， pgcs ） ，培養在添加人重組白血病抑制因子（ lif ） 、人重組堿性成纖維細胞生長因子（ bfgf ）和forskolin的小鼠飼養層細胞上。
  3. Effects of cytokine combinations and calcium ionophore on differentiation of dendritic cells from acute myeloid leukemia cells

    組合性細胞因子與鈣離子載體誘導急性髓系白血病細胞向樹突狀細胞分化中的作用
  4. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  5. All of these indicated that arsenic trioxide was a powerful chemotherapeutic agent and the cells in the treatment group were induced to apoptosis. to further understand the molecular mechanisms of arsenic trioxide treatment in the induced cellular apoptosis, we applied the restriction display - pcr ( rd - pcr ) technique combined with polyacrylamide gel electrophoresis and sliver staining techniques to separate the differentially expressed genes

    為了進一步闡述as _ 2o _ 3作用的分子生物學機制，我們首先應用rd - pcr技術，結合聚丙烯酰胺凝膠電泳和銀染技術分離和顯示了as _ 2o _ 3作用k562細胞前後的cdna片段，發現了11個差異表達的基因片段。
  6. Studies revealed that p - catenin dissociated from ccc and translocated into free catenin pool in cytosol after it has been phosphorylated at tyrosine or serine residues, and in this situation, the ccc has been disrupted and cell adhesion function disturbed. a large amount of the free p - catenins in the cytosol can be degraded by the tumor suppressor apc, and the remains translocate into nucleus and bind to transcriptional factor tcf / lef in the nucleus and then promote cell proliferation related gene or anti - apoptosis gene transcription

    當-連環蛋白酪氨酸或絲氨酸殘基磷酸化后，就與ajs發生解離而游離到細胞漿中，此時細胞的粘附功能也發生障礙，游離到胞漿中的-連環蛋白，一部分被抑癌因子apc降解，一部分則轉移到細胞核內，與核內的轉錄因子tcf lef結合，啟動與細胞增殖有關的基因轉錄。
  7. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  8. The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression, and guard cell - specific promoter is becoming one of the best choices

    因此有必要對氣孔的開關進行調控。氣孔運動的分子調控離不開高效的基因開關系統，保衛細胞特異性表達的啟動子近幾年倍受關注。
  9. Segregation the separation of the two alleles of a gene into different gametes, brought about by the separation of homologous chromosomes at anaphase 1 of meiosis

    分離：同源染色體在細胞減數分裂後期中向細胞極遷移，使等位基因最終分向不同配子。
  10. Enriched with vitamin e, egf. ha and other nourishment, this and lotion protects your hands from the cold wind, dry weather and other harm. the special nall - rebuilding formula can make your nails healthy and shining. it can also solve the common skin problems like crackle, decortications, allergy. etc. the moisturizing component effectively keeps the balance of water and makes your hands soft for 24 hours

    蘊含維他命e ，表皮細胞生長素（ egf ） ，特效保濕因子及透明質酸ha等多種滋養成分，能有效隔離風沙，寒冬及乾燥等外界環境及刺激物對手部皮膚造成的傷害，獨特的修護指甲配方，令指甲柔韌潤澤，不易折斷，同時防止皮膚乾燥，皺裂，冬日爆裂脫皮及潔劑敏感等手部皮膚常見問題，高效保濕因子有效鎖住肌膚水分，防些水分流失，令雙手整日保持細膩。
  11. By the compounds of submandibular gland cells and collagen sponges. we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges, the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge. our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg

    在本研究中，以初步探討體外頜下腺細胞與膠原海綿支架相互作用為目的，採用體外分離培養sd大鼠頜下腺細胞，然後接種于膠原海綿支架上體外復合培養的方法；從不同接種細胞濃度對細胞一支架復合物影響，同一接種細胞濃度在不同孔隙率的支架上黏附、增殖的情況及表皮生長因子（ egf ）對頜下腺細胞的促增殖作用，促細胞在支架上黏附等三方面入手，初步研究了頜下腺細胞與膠原海綿相互作用的影響因素，為進一步在體外及體內構建較為理想的組織工程化頜下腺提供理論參數和實驗依據。
  12. The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin

    從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。
  13. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  14. Cell detaching factor

    細胞分離因子
  15. The implantion percentage of embryos and the cloned es - like cells were low

    本實驗研究了胚齡、生長因子、飼養層對山羊es細胞分離和克隆的影響。
  16. Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities

    因此將這些蛋白包被、固定到材料表面，觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節，並從以下方面進行探討： （ 1 ）採用不同原代細胞分離方法，研究其對mscs細胞的生物學特性影響。 （ 2 ）檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 （ 3 ）分別採用型膠原及纖維粘連蛋白（ fibronectin ， fn ）包被聚乙醇酸-乳酸共聚物（ poly （ 1actide - co - glycolide ） ， plga ）膜及多孔塊型plga材料，觀察細胞在單層或三維培養狀態下，型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。
  17. A cdna labrary was constructed by using the floral meristem materials from the explants cultured on the medium containing hormones for 5 days and 10 days. screenings of cdna library was carried out by a microarray technique. two probes were used

    本研究利用風信子離體實驗系統，從分離生長素和細胞分裂素誘導響應的基因著手，為初步理解兩種激素在花發育過程中的作用機理提供新資料。
  18. To gain a molecular understanding of tumor angiogenesis, we compared gene expression patterns of endothelial cells derived from blood vessels of normal and malignant colorectal tissues

    為了能夠在分子水平上了解腫瘤的血管造影照片，我們對比了從正常和惡性結腸癌組織的血管分離到的上皮細胞的基因表達模式。
  19. Adipokines, the bioactive factors derived mainly from adipocytes, regulate pancreatic - cell function including insulin secretion, gene expression and apoptosis. in this review, we propose that adipokines influence - cell function through three interdependent pathways. the first is through regulating lipid and glucose metabolism in - cells. the second implicates the change of ion channel opening and closing in - cells. the third pathway is via the modification of insulin sensitivity of - cells. the endocrine function of adipocytes is dynamic, and the secretion of various adipokines changes under different metabolic conditions. during the progression from the normal state to obesity and to type 2 diabetes, adipokines contribute to the occurrence and development of - cell dysfunction in type 2 diabetes

    脂肪因子包括脂肪細胞分泌的多種活性因子，它們通過內分泌方式調節胰島細胞的胰島素分泌、基因表達以及細胞凋亡等多方面的功能.本文提出脂肪因子影響胰島細胞功能主要通過三條相互聯系的途徑而實現.第一是調節細胞內葡萄糖和脂肪的代謝;第二是影響細胞離子通道的活性;第三是改變細胞本身的胰島素敏感性.脂肪細胞的內分泌功能是一個動態過程，在不同的代謝狀態下，各脂肪因子的分泌發生不同變化.從正常代謝狀態發展到肥胖以及2型糖尿病的過程中，脂肪因子參與了胰島細胞功能障礙的發生與發展
  20. Abstract : adipokines, the bioactive factors derived mainly from adipocytes, regulate pancreatic - cell function including insulin secretion, gene expression and apoptosis. in this review, we propose that adipokines influence - cell function through three interdependent pathways. the first is through regulating lipid and glucose metabolism in - cells. the second implicates the change of ion channel opening and closing in - cells. the third pathway is via the modification of insulin sensitivity of - cells. the endocrine function of adipocytes is dynamic, and the secretion of various adipokines changes under different metabolic conditions. during the progression from the normal state to obesity and to type 2 diabetes, adipokines contribute to the occurrence and development of - cell dysfunction in type 2 diabetes

    文摘:脂肪因子包括脂肪細胞分泌的多種活性因子，它們通過內分泌方式調節胰島細胞的胰島素分泌、基因表達以及細胞凋亡等多方面的功能.本文提出脂肪因子影響胰島細胞功能主要通過三條相互聯系的途徑而實現.第一是調節細胞內葡萄糖和脂肪的代謝;第二是影響細胞離子通道的活性;第三是改變細胞本身的胰島素敏感性.脂肪細胞的內分泌功能是一個動態過程，在不同的代謝狀態下，各脂肪因子的分泌發生不同變化.從正常代謝狀態發展到肥胖以及2型糖尿病的過程中，脂肪因子參與了胰島細胞功能障礙的發生與發展
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