細胞外毒素 的英文怎麼說

中文拼音 [xìbāowàidúsù]
細胞外毒素 英文
extracellular toxin
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 外 : Ⅰ名詞1 （外面） outside; external side 2 （外國） foreign country 3 （以外） besides; beyond; in ...
  • 毒 : Ⅰ名詞1 （對生物體有害的性質或物質; 毒物） poison; toxin 2 （毒品） drug; narcotics 3 （姓氏） a s...
  • 素 : Ⅰ形容詞1 （本色; 白色） white 2 （顏色單純） plain; simple; quiet 3 （本來的; 原有的） native Ⅱ名...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  • 毒素 : 1. [生物學] （有機體產生的有毒物質） toxin 2. （言論或著作中有腐蝕作用的成分） poison
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  3. The toxin may be formed within the pathogen and released when it dies ( endotoxin ), or secreted through its cell wall ( exotoxin )

    在病原體內形成，當病原體裂解時被釋放的毒素為內毒素；由病原體通過細胞壁向外分泌的毒素為外毒素。
  4. Abstract : this paper describes the role of the sialylsugar chains on cell surface in the attacking process of the influenza virus, and also binding specificity of hemagglutinin of the influenza virus to the sialylsugar chains

    文摘:介紹了細胞外表面上含唾液酸的糖鏈在流感病毒侵襲細胞中的作用以及糖鏈與流感病毒表面糖蛋白-血凝素結合的特異性。
  5. Their outside individual nucleus cell is in week blood the leucocyte interferon that small amount produces only after virus is atttacked

    他們的外周血單個核細胞在病毒攻擊后僅產生低量的白細胞干擾素。
  6. Botulinum neurotoxin serotype a produced by the clostridium botulinum, are the most lethal substances known. a single molicule can abolish the function of a nerve cell. the toxins exert their action by blocking the release of the neurotransmitter, acetylcholie, at the neuromuscular junction, the blockage of bont / a makes neuromuscular weakness and flsccid paralysis, and lead to respiratory failure and death. botulinum is a serious public heath problem in developing countries around the world

    A型肉毒毒素（ botulinumneurotoxinserotypea ， bont a ）是由肉毒梭菌產生的一種強烈的外毒素，是目前已知最毒的天然物質，一個單分子的肉毒素分子就能阻斷一個神經細胞的功能，通過阻斷運動神經末梢乙酰膽堿的釋放導致機體發生癱瘓，嚴重者發生呼吸衰竭而死亡。
  7. Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control

    目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響，探討內毒素影響體外受精結局的環節及可能的機制，確定三個體外培養系統對內毒素的敏感性，以期為臨床和實驗室質量控制提供參考。
  8. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  9. Ammonium chloride is rapidly absorbed from the gastro - intestinal tract. the ammonium ion is converted in urea into the liver ; the anion thus liberated into the blood stream and extracellular fluids causes a metabolic acidosis and decreases the ph of the urine ; this is followed by a transient diuresis

    氯化銨能迅速地從胃腸道被吸收。在肝臟氨鹽基離子被轉換成尿素，陰離子因而被解放入血液和細胞外流體導致代謝性的酸中毒，使尿液變酸，形成短暫的多尿。
  10. The biological activity of purified lt 27 was tested with the assay system, and its biological activity was 2 - 3 107 iu / mg. pro. the cytotoxicity of purified lt 27 was in the same level with rhtnfp and lt international standard. it shows that lt deletion could keep its high cytotoxicity towards tumour cell l929 in vitro after 27 amino acids deleted from its n - terminal

    用建立的淋巴毒素生物活性測定方法對上述純化的淋巴毒素缺失體lt 27的生物活性進行檢測，測得其比活為2一3xl口iu / mg . proo純化的淋巴毒素缺失體lt 27的生物活性與rhtnfp和淋巴毒素國標標準品的生物活性大致相當，表明lt經n端缺失27個氨基酸后仍能保持很高的體外腫瘤細胞毒活性。
  11. It is thought that extracellular gsh protects the cells from oxidants released by inflammatory cells whilst intracellular gsh neutralizes the ros produced as a result of normal cell metabolism, as well as detoxifying xenobiotics ( 4 )

    研究者認為細胞外的谷胱甘肽可保戶細胞免受發炎細胞所釋放氧化劑的攻擊，而細胞內的谷胱甘肽可中和正常細胞代謝作用以及共棲生物解毒作用所產生的具反應性氧核素。
  12. Interaction of beta - carotene on rat pneumocytes exposed to cigarette smoking particle extracts

    胡蘿卜素與香煙煙氣顆粒物對體外大鼠肺細胞毒性的交互作用
  13. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  14. The relationship between the efficacy of interferon - alpha treatment and the variation of perforin protein expression in peripheral blood mononuclear cells in chronic hepatitis b patients

    慢性乙型肝炎患者外周血單個核細胞穿孔素表達與干擾素抗病毒療效的關系
  15. In the light of established surgical techniques and higher efficacy of modern radiotherapy, cytotoxic and hormonal drugs in breast cancer, a population survey by the faculty of medicine of cuhk revealed the public still have many misconceptions over breast cancer management and knowledge of mmg is not enough

    盡管外科技術日趨成熟，現代放射療法及乳癌的細胞毒素和激素藥物的效能提高，中文大學醫學院腫瘤學系的楊明明教授、關永康教授和徐仲
  16. This peptide is derived from proteolytic processing of the amyloid precursor protein ( app ). an increasing ' amount of experiments show that a p can induce various toxic reaction in brain, thus play a causal role in the pathogenesis of alzheimer ' s disease ( ad ). in the meantime, the incapability of neurons to regenerate in ad patients means that it may inhibit the proliferation, differentiation and even survival of nscs

    3 .體外培養14天後，取部分細胞，加入不含bfgf僅含egf （ 20n留ml ）的無血清培養基，分為三組:對照組; ap25一35毒性組加入20umol /的凝聚態a目25一35 :雌激素保護組同時加入zoumolz的凝聚態ap25一35和10一7mo比17一p雌二醇。
  17. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  18. The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin. the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo. angiostatin showed significantly inhibitory effect on endothelial cells

    重組病毒以moi = 10感染家蠶細胞（ 2 10 ~ 6個細胞/ 5ml ）和家蠶5齡幼蟲，表達產物用體外培養的人臍靜脈血管內皮細胞（ ecv304 ）及體內雞胚尿囊膜（ cam ）新生血管實驗檢測其抑制活性： （一）血管抑素可明顯抑制體外培養的內皮細胞增殖。
  19. Phototherapy technology : light energy can get into skin as deep as 10mm through blood circulation, and produce hot utility to expand capillary, accelerate the exchange of electrolyte and body fluid, consuming fat and enhancing functions of cells and blood vessels, discharging excessive water, toxin and pigment out of body

    光療技術：能量作用於表皮時，通過血液循環使得熱能進入皮膚深層組織，可直接深入皮膚10毫米左右，其熱效應可使微血管擴張，加速電解質與體液的交換，使得脂肪在皮下燃燒並消耗，加強血管和細胞的功能，皮膚吸收熱量后，毛孔打開，可使多餘水份、毒素、色素排出體外。
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