細菌組蛋白 的英文怎麼說

中文拼音 [xìjūnzǔdànbái]
細菌組蛋白 英文
hu protein
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da

    採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。
  4. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  5. These issues are essential to the understanding of the nature of cell cycle control, and to elucidating the roles of the histone acetylation modification in cell cycle regulation in eukaryotes. in this thesis, we studied the mechanisms of histone acetylation in cell cycle regulation in physarum polycephalum, a naturally synchronized slime mold. the results of this study confirmed that histone acetylation changed the expression of proteins related with the regulation of checkpoint conversion in cell cycle

    初步確定了組蛋白乙酰化在多頭絨泡菌細胞周期各轉換點的調控過程中對細胞周期調控相關蛋白表達水平的調節作用；了解了組蛋白乙酰化修飾對細胞周期各轉換點調控的作用途徑；建立了組蛋白乙酰化修飾對多頭絨泡菌細胞周期調控機制的基本模型。
  6. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  7. After the protein was electrophoresised and purified, the protein activity was detected by elisa, the protein activity of vp1 is higher than vp0 vp3. at last, the activity of vp1 made in our lab was detected with the agentia made in our lab

    將陽性重組子轉化到大腸桿菌er2566細菌內，用ipig進行誘導表達蛋白，蛋白經電泳、純化，然後用elisa方法檢測蛋白活性， vp1蛋白活性相對高於vp0 、 vp3 。
  8. The biological functions testified include : enhance iron ion assimilation of epithelial cell of intestine and equilibrate body iron concentration ; broad spectrum of antiviral activity, antibacterial activity and antifungal activity ; modulate marrow cell production and growth ; help to mature and regulate a number of immune cells throughout the body, thus boost body immune ability ; prevents " free iron " from forming free - radicals ; supress tumour growth and prevent tumour formation in animal models

    乳鐵蛋白是一種糖蛋白，為轉鐵蛋白家族的一員，在人和哺乳動物的許多器官與組織中廣泛分佈。乳鐵蛋白具有多種生物學功能，這些功能包括：促進人體腸道對鐵的吸收及調節體內鐵的平衡；廣譜抗菌（細菌和真菌） 、抗病毒感染作用等。
  9. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  10. Studies of the crystal structure of endostatin have shown a compact globular fold, with one face particularly rich in arginine residues acting as a heparin - binding epitope, this site was recently shown to be involved in the inhibition of induced angiogenesis. experimental studies show that recombinant endostatin specifically inhibits the proliferation of endothelial cells in a dosedependent fashion. recombinant endostatin from bacteria is largely insoluble, but still efficient in arresting tu mor growth after injection into mice. intermittent therapy with recombinant bacterially produced endostatin reduces several experimental tumors, including lewis lung carcinoma, to a dormant state. no sign of drug induced resistance has been reported and, in the original study, the treatment dormancy appeared to persist even when therapy was discontinued. sowe regard endostatin as a promising anti - tumor drug

    許多研究表明重組內皮抑素特異性抑制內皮細胞增殖，而且這種抑制作用呈劑量依賴性。細菌表達產物內皮抑素大部分以不溶形式存在，將這種混懸液注射治療老鼠仍可以抑制腫瘤生長。于小鼠皮下重復注射內皮抑素重組蛋白，幾乎完全抑制鼠lewis肺癌等多種腫瘤生長，並無耐藥性產生，即使中斷治療腫瘤也不再復發。
  11. Functions : this product contains “ antifungal active protein ” with optimal and strong sterilization effect, which could strengthen the immunity of body fluid, activate the macrophage, strengthen the phagocytosis ability of macrophage, strengthen the body immunity, restrain the growth, pervasion and transfer of abnormal cells, the product has strong sterilization effect and could strengthen the disease resistance ; it is remarkably effective in improving the immunity and improving the infirm constitution of pets ; the amino acid content and composition in the product are moderate and rational, with the characteristics of strong palatability, nutrition balance and immune element abundance, etc

    功能：本產品中擁有極佳強烈殺菌作用的「抗菌活性蛋白」 ，能增強體液免疫功能，活化巨噬細胞，增強其吞噬能力，可增強機體免疫力，抑制非正常細胞生長、擴散和轉移，具有強烈的殺毒作用，增強抗病性；對提高寵物免疫力，改善虛弱體質有顯著效果；其中氨基酸含量適中、組成合理，具有適口性強、營養均衡和免疫物質豐富等特點。
  12. It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating

    抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。
  13. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。
  14. The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management

    重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。
  15. By treating the cells in s, g2 phase and prophase with histone deacetylase inhibitor tsa, and through the application of microscopic observation and western - blotting, we demonstrated that histone acetylation modification played important roles in the cell cycle regulation in physarum polycephalum, affecting the normal crossover of the checkpoints of s / g2, g2 / m and mitosis exit

    這些炎白的表達水平具有細胞周期依賴性，隨著細胞周期的進行而發生變化。 tsa處理引起的多頭絨泡菌s期、 gz期和前期細胞內組蛋白h3乙酚化水平的提高，改變了細胞內類cyclinbi蛋白、類。
  16. Centrifuge for 10min at full speed in a tabletop microcentrifuge. the whitish pellet contains proteins and the bacterial genomic dna

    用桌上型微量離心機以最高速離心10分鐘。白色沈澱物含有蛋白質與細菌基因組dna 。
  17. The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf

    誘導后，培養液上清通過western - blotting分析證明，轉染細胞表達並分泌出人乳鐵蛋白，分子量為34kd ； elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞；抗菌實驗表明，所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用，而且比人乳鐵蛋白標準品作用更強。
  18. 6 hours later, the amount of the expression yield of cp accounting for the total protein of the cells was 28. 9 % ( about 2mg / ml cp ). after sds - page, the recovery of cp added the same volume of incomp lete freund adjuvant emulsified completely, injected rabbits by subcutaneous injection. every rabbit was injected 2ml emulsified antigen ( 300ug cp ) once a week

    重組表達載體導入宿主菌e . colibl21 （ de3 ） ，用終濃度為1mmol / liptg誘導， 37培養6小時后， cp基因的表達量達到最高，每毫升菌液含目的蛋白的量約為2mg ； uvi光密度掃描分析表明cp基因的表達量占細菌總蛋白的28 . 9 。
  19. This paper reviews the recent advances in yeast expression systems used in recombinant protein drugs produced by gene engineering, such as the selection of yeast vector system and yeast expression strain, construction of multi - copy strain, the high yeast cell density culture, the recent situation of yeast system applied in expression gene, defect of yeast expression system and countermeasure, etc

    摘要本文主要從酵母載體系統的選擇、酵母表達宿主的選擇、多拷貝菌株的構建、高密度發酵培養酵母細胞、應用酵母系統表達外源基因的研究現狀、酵母表達系統的缺陷及對策等幾個方面綜述了近年來酵母表達體系在基因工程重組蛋白藥物開發方面的研究進展。
  20. In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg

    同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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